百日咳杆菌

兴奋性氨基酸和特异性受体对大鼠百日咳杆菌脑损伤的作用

Effects of excitatory amino acids and its specific receptor on bordetella pertussis brain injury of rats

方法向大鼠左颈内动脉注射百日咳杆菌悬浮液（0.2ml／kg体重），诱发感染性脑损伤模型。

Methods An IBI model was induced by injection of bordetella pertussis suspension ( BPS ) via the left internal carotid arteries of the rats .

ELISA在检测百日咳杆菌免疫原中的应用

The application of ELISA in the assessment of B. pertussis immunogen

方法应用豚鼠脊髓匀浆加完全弗（氏）佐剂和百日咳杆菌免疫Wistar大鼠。

Methods Each Wistar rat was injected with Cavy 's spinal cord homogenate , mycobacteria and IFA , and Bordetella Pertussis bacilli .

A组大鼠用卵蛋白（OA）辅以百日咳杆菌菌苗和氢氧化铝为佐剂注射致敏。

The rats in group A were sensitized by injection of ovalbumin ( OA ) together with aluminum hydroxide and Bordetella pertussis as adjuvants .

百日咳杆菌LPF和FHA的纯化及其特性的研究

Studies on Separation , Purification and Properties of LPF and FHA from B. Pertussis

目的：探讨兴奋性氨基酸、N甲基D天冬氨酸受体（NMDAR）在大鼠百日咳杆菌脑损伤中的变化及作用机制。

Objective : To explore changes and mechanism of EAAs and its specific N-methyl-D-aspartate receptor ( NMDAR ) on bordetella pertussis brain injury in rats .

方法采用PCR法从百日咳杆菌染色体中获得S1亚单位的结构基因并克隆到质粒pCR2.1-TOPO中，转化宿主菌DH5α和TOPO10。

Methods The structural gene encoding pertussis toxin SI subunit of Bordetella pertussis was amplified by PCR , cloned into vector pCR 2.1 - TOPO , and transformed to E.

应用TritonX-100抽提和SephadexG-100层析获得百日咳杆菌外膜蛋白（OMP）及其5个组分（OMP1～OMP5）。

The outer membrane protein ( OMP ) and its fractions ( OMP_1 ~ OMP_5 ) of B. pertussis were obtained through Triton X-100 treatment and Sephadex G-100 column chromatography .

为探讨中医不同治疗对哮喘的疗效与机理，将SD大鼠用卵蛋白、灭活百日咳杆菌、氢氧化铝干粉混合液腹腔注射以致敏后，用2%卵蛋白雾化吸入诱发哮喘发作。

To investigate the clinical efficacy of different treatments in treating asthma and its mechanism , the SD rats were sensitized by intra-abdominal injection of mixed ovalbumin , inactivated bacillus pertussis and aluminum hydroxide , and then induced to have asthma by inhaling 2 % ovalbumin .

方法分别克隆疫苗株的S1和Prn基因至PGEM-Teasy载体、进行测序，并与GenBank中收录的百日咳杆菌S1和Prn蛋白基因序列进行比较、分析。

Methods Full length of S1 and Prn genes of three vaccine strains were amplified by PCR . The PCR amplicons were sequenced after being cloned into PGEM-T easy vector and the results were compared with those of Bordetella pertussis strains in GenBank .

哮喘模型组、心理应激哮喘组、心理应激哮喘中药组分别腹腔注射致敏液（含卵蛋白100mg、氢氧化铝100mg、百日咳杆菌5×109个）0.8mL，1次/d。

Mice in the asthma model group , psychological stress asthma group and TCM group were intraperitoneally injected with 0.8 mL of sensitization fluid ( containing 100 mg of egg protein , 100 mg of aluminum hydroxide , 5 × 109 bacillus pertussis respectively ) once a day .

百日咳杆菌，克隆号：5111，单克隆抗体抗。

LPS , Bordetella pertussis , Clone : 5111 , Mab anti .

百日咳杆菌丝状血凝素的纯制及其生物学特性的研究

Purification and Characterization of Filamentous Hemagglutinin from Bordetella Pertussis Culture

百日咳杆菌诱导大鼠急性感染性脑水肿模型

A new model of acute brain edema induced by pertussis bacilli in rats

百日咳杆菌外膜蛋白及其组分的毒性与免疫原性

Toxicity and antigenicity of the outer membrane protein and Its fractions of bordetella pertussis

百日咳杆菌粘着素的分子克隆、表达、纯化及生物学特性研究

Molecular Cloning , Expressing , Purification and Characterization of Recombinant Pertactin of Bordetella Pertussis

多价气性坏疽抗毒素百日咳杆菌去内毒素及保护性抗原的纯化

Studies on the removal of endotoxin and purification of protective antigens of Bordetella pertussis

某些可溶性百日咳杆菌提取物，可证明用之有效，也不产生严重副作用。

Certain soluble extracts of B pertussis may prove to be effective without engendering serious side effects .

结果所有111份鼻咽拭子中，8例百日咳杆菌培养阳性，阳性率为72%。

Results There were 8 ( 7 2 % ) cases which had culture positive of B.pertussis among 111 swabs .

脑水肿模型组（一侧大鼠颈总动脉注射百日咳杆菌250亿/kg）；

Group D : brain edema model rats established by unilateral common carotid artery injection of Bacillus pertussis 250 million / kg ;

除a组外，采用腹腔注射卵白蛋白和灭活百日咳杆菌的方法，制成大鼠哮喘模型。

Beside group a , egg albumin was injected into the abdominal cavity plus the method to kill Bordetella pertussis , to create asthma model in the rats .

致病菌为百日咳博尔德氏杆菌，潜伏期一周，类似普通感冒，伴有短促的干咳。

Caused by the bacterium Bordetella pertussis , it initially resembles a cold with a short dry cough .

这些年来，我们总是专门致力于对抗其中一种病菌，而另一种病菌则伺机活跃起来，以致接种疫苗会促使副百日咳博多氏杆菌的肺部感染率上升到正常情况下的40倍。

In all these years of exclusively pounding away at one of the causes , the second type of bacteria has been flourishing , to the point that receiving the vaccine causes B. parapertussis lung infections to grow 40 times as large as they would normally .

百日咳是主要由百日咳杆菌引起的一种传染性强的急性呼吸系统疾病，主要见于发展中国家未接种相应疫苗的儿童。

Pertussis is an acute infectious disease of the respiratory system , which is mainly caused by Bordetella pertussis and found in non-immunised children of developing countries .

百日咳是一种由百日咳杆菌引起的严重的呼吸系统传染病，百日咳疫苗是预防百日咳最有效和最经济的手段。

Whooping cough is a serious respiratory disease caused by Bordetella pertussis .

百日咳有两种病原菌，即百日咳博多氏杆菌和副百日咳博多氏杆菌，但百日咳疫苗——DTaP疫苗——却只针对百日咳博多氏杆菌这一种病原菌。

Whooping cough is caused by two types of bacteria , Bordatella pertussis and Bortatella parapertussis , but the vaccine - the DTaP - is only designed to fight the first one , B. Pertussis .