生孢梭菌

夏孢生枝孢C.对生孢梭菌CFU计数培养基的验证试验

Verifying the Efficacy of CFU Counting Culture Medium for Clostridium sporogenes

将生孢梭菌的新鲜培养物置无菌离心管中，500r·min~（-1）离心5min。

Put the fresh medium with Clostridium sporogenes into centrifugal tube , at 500 r · min ~ ( - 1 ) for 5 min.

输液灭菌工艺中F0值的研究生孢梭菌孢子作为生物指示剂用于残存概率灭菌工艺验证的可行性

The F0 Research in the Process of Solution Sterilization Use of Clostridium Sporogenes Spores as Biological Indicator During Sterilization Cycle Validations

优选18h进行CFU/mL计数并观察菌落形态，优选琼脂浓度为0.5%的上述培养基作为生孢梭菌CFU计数培养基并与其他5个培养基进行生孢梭菌CFU计数的比较试验。

Preferred to choose 18 h for CFU Count and colony observation , and to choose the researching medium with 0.5 % agar as the CFU counting culture medium for Clostridium sporogenes compared with other 5 media .

《中国药典》1995年版二部附录无菌检查法，新增加收载生孢梭菌（Clostridiumsporogenes）做为抗厌氧菌药物注射液无菌检查的阳性对照菌。

Clostridium Sporogenes has been added to China Pharmacopeia 1995 edition as the standard positive control bacterium in the sterility test of injections .

目的：研制一种适宜生孢子梭状芽孢杆菌（简称生孢梭菌）菌落形成单位（ColonyFormingUnits，CFU）计数的微生物培养基（简称培养基），使其CFU计数能够接近最大活菌数。

Objective : To develop a kind of appropriate CFU ( Colony Forming Units ) counting culture medium for Clostridium sporogenes , make CFU Count be approximate to the most amount of practical alive bacteria . Methods : Inoculated Clostridium sporogenes on fluid thioglycollate culture medium ( without agar ) .