牛肉膏

本试验比较了甘氨酸缓冲液、牛肉膏溶液、磷酸缓冲液3种最为常用的洗脱液在不同pH条件下（7、9.5、11）的最佳洗脱效果。

In this study , we compared the best elution efficiency among glycine buffer , beef extract solution and phosphate buffer in different pH ( 7 , 9.5 , and 11 ) .

又采用四因素五水平通用旋转设计，研究了大豆蛋白胨添加量、牛肉膏添加量、酵母膏添加量和基质pH值对菌体浓度的影响。

And he influence of Soya peptone addition , Beef Extract addition , Yeast Extract addition and the initial pH of the media concentration on the living bacteria number by four elements quadratic currents quadratic current revolving design .

向PDA培养基中加入膨胀石墨观察黑曲霉的生长，向牛肉膏蛋白胨培养基中加入膨胀石墨观察金黄色葡萄球菌的生长。

Expanded graphite were added in PDA medium to observe the growth of Aspergillus niger , and in beef extract peptone medium to observe the growth of Staphylococcus aureus .

粘质沙雷氏菌（Serratiamarcescens）9-1、9-2、16-13菌株，在牛肉膏蛋白胨培养基上生长很好，产生红色菌落。

Three strains ( 9-1,9-2,16-1 ) of Serratia marcescens were grown very well and produced bright red pigment colony in beef-protein medium .

牛肉膏蛋白胨是较好的氮源物质，72h干菌丝产量为8.87mg/mL，是其他氮源的3.5~6.0倍。

The dry mycelia biomass in Beef peptone media could reach 8.87 mg / mL in 72 h that was 3.56.0 times than the production in other nitrogenous substance media .

结果：确定了最佳培养基为：牛肉膏为2.5%、KBr为0.01%、盐浓度为6%；

Results : The results showed that the optimal fermentation medium were obtained as follow : NaCl 6 % , beef extract 2.5 % , potassium bromide 0.01 % ;

菌株ZP在牛肉膏蛋白胨固体培养基上菌落为深绿色，产生水溶性绿色素弥漫整个平板，革兰氏染色阴性，呈杆状，无鞭毛。

The colony of ZP is bottle green on the solid culture medium of beef extract , because the bacterial strain ZP is able to produce water-solubility pigment .

实验结果表明，牛肉膏蛋白胨培养基、YPD培养基、抗菌素淀粉培养基分别对糟醅中细菌、酵母菌和霉菌具有较好的分离培养效果。

The results of experiments showed that Beef extract-peptone medium , YPD medium , antibiotic starch medium could isolate bacterium , yeasts and mold very well , respectively .

t-3的麦芽复合汁增菌培养基最佳配比为：在10%的麦芽汁中添加0.5%大豆蛋白胨、0.5%酵母膏、1%牛肉膏、0.2%K2HPO4；在麦芽复合汁增菌培养基中，S。

The optimum enrichment medium obtained by orthogonality test consist of adding 0.5 % Soya peptone , 0.5 % Yeast Extract , 1 % Beef Extract , 0.2 % K_2HPO_4 in the 10 % wort medium .

采用正交试验设计，初步地确定了菌株SHL-4产酶培养基组分，即蛋白胨0.5%、牛肉膏1.5%。

Through an orthogonal test , the producing protease culture medium of the SHL-4 strain was confirmed preliminarily , namely 0.5 % of peptone , 1.5 % of beef extract .

较适合的种子培养基为牛肉膏大豆蛋白胨培养基；

The suitable seed culture medium was beef extract soya peptone medium ;

玉米秸秆、牛肉膏可明显促进纤维素酶的合成。

Corn straw and beef grease can obviously promote the synthesis of cellulase .

最佳碳氮源分别为葡萄糖和牛肉膏。

The best carbon and nitrogen source were glucose and beef extract respectively .

蛋白胨和牛肉膏溶液对厚垣孢子和分生孢子萌发的促进作用最大；

Both chlamydospore and conidium germinated best in the peptone and beef extract solution .

结果表明，黄伞菌丝深层发酵适宜的碳源为葡萄糖，适宜的氮源为牛肉膏；

The results showed that the optimal carbon source for P.adiposa was glucose , the best nitrogen source for the strain was beef extract .

酵母粉、蛋白胨、牛肉膏等氮源有较强的促进作用，以酵母粉最为显著，而尿素的作用较低。

For nitrogen source , yeast extract , peptone , beef ointment all had a strong role in promoting the xylanase , but urea .

本研究对Ⅶ号菌株的培养基条件进行了探究，结果发现，Ⅶ号菌株的最佳碳源为牛肉膏，最佳氮源为蛋白胨。

The cultural medium was studied in this research , the optimum carbon source was beef extract , and the optimum nitrogen source was peptone .

为了诱导愈伤组织分化植株，突变细胞移入牛肉膏分化培养基（每升含2毫克苄基嘌呤和0.5毫克萘乙酸）。

In order to regenerate plants , mutant callus was inoculated onto a beef medium extract containing 2 mg benzyladenine and 0.5 mg NAA per liter .

最有利于菌丝生长的碳源为麦芽糖和葡萄糖，氮源为酵母膏、蛋白胨、牛肉膏。

Among the tested carbon and nitrogen sources , glucose , maltose , yeast extract , peptone , beef extract were favorable to the growth of mycelia .

在1%牛肉膏培养基（没有无机氮的）上筛选突变细胞。

Selec - tion of nutrient mutant cells was carried out by planting the culture in a medium with 1 % beef extract without any inorganic nitrogen .

结果表明，适宜的碳源是葡萄糖、果糖、红糖、乳糖，适宜的氮源是蛋白胨、麦麸、牛肉膏、酵母膏；

The result showed that the optimumcarbon sources were glucose , fructose , brown-sugar , lactose and the nitrogen sources werepeptone , wheat-bran , beef-extract paste , yeast-extract paste .

突变细胞虽能在1%牛肉膏培养基上生长，但达4%时生长也停止了。

Without beef extract , the normal cells were still viable but grew very slowly . The mutant cells were able to grow at 1 % while stopped at 4 % .

把突变细胞转入没有牛肉膏的无机氮培养基上，经6代42天培养，仍能生长，表明突变型的特性是比较稳定的。

This trait was stable as mutant cells grown at inorganic nitrogen medium without beef extract for 42 days ( subcultures for 6 passages ) were still adapted to beef extract medium .

结果表明，荷叶离褶伞菌丝生长最适碳源是果糖，最适氮源是牛肉膏，最适碳氮比是20∶1～1∶30。

The results showed that the best C-sources is fructose , the best N-sources is extract of beef , the best ratio of carbon to nitrogen is20 ∶ 1 ~ 30 ∶ 1 .

菌丝生长对氮素要求也呈现明显的差异，对KN03、牛肉膏和酵母膏利用最好，而对硫酸铵和氯化铵利用最差。

Nitrogen requirements of mycelial ' growth showed significant differences . The pathogen liked KNO3 , beef extract and yeast extract the best , and the worst were ammonium sulfate and ammonium chloride .

单纯用牛肉膏蛋白胨培养基结合平板涂布计数法测定好氧细菌数量，不能真实反映窖内好氧细菌的真实情况。

Moreover , single use of beef extract peptone medium combined with spread plate count method to measure the quantity of aerobic bacteria could not reflect the real status of aerobic bacteria in pits .

其中1~菌在牛肉膏固体培养基上呈桃红色至红色，革兰氏阴性球菌，呈球状，无鞭毛，直径约0.5μm。

Among those bacterial consortium , the colony of 1 ~ # is peachblow on the solid culture medium of beef extract , and is gram - negative , globular and non-flagella , 0.5 μ m in diameter ;

最佳理化处理条件是：碳源为碱超声处理的棉秆、氮源为牛肉膏、发酵温度为40℃、发酵时间为7天。

The best physical chemic pretreatment condition is that the cotton straw after ultrasonic treatment with alkali as C source , beef extract as N source , 40 ℃ as ferment temperature and 7 days as ferment time .

在对F121112菌株的发酵条件进行优化后，F121112菌株的最佳摇瓶发酵条件是：发酵培养基配方为蛋白胨0.4%、酵母提取物0.5%，牛肉膏0.4%，葡萄糖0.5%；

The fermentation conditions have been further examined in the current work . The results showed that the best fermentation conditions was : the optimum fermentation mediums was composed of 0.4 % peptone . 0.5 % yeast extraction .