焦磷酸测序

结论：焦磷酸测序技术是一种简便、高效、高通量、高灵敏度、高准确度的HPV分型检测方法。

The pyrosequencing is simple , high efficiency , high flux , high-throughput high sensitivity and high accuracy method of typing HPV .

与传统Sanger法相比，焦磷酸测序技术是一种实时的DNA测序技术，具有快速、高效、成本低的特点。

Pyrosequencing is a real-time DNA sequencing method , which is fast , efficient and low cost , comparing to that based on Sanger reaction .

用焦磷酸测序技术研究猪线粒体细胞色素B基因单倍型

Porcine Mitochondrial Cytochrome B Gene Haplotypes Detection by Pyrosequencing

优化单链DNA模板的制备条件和焦磷酸测序分析的参数。

Single-stranded DNA template preparation and pyrosequencing analysis of the parameters were optimized .

焦磷酸测序检测RARβ2基因启动子甲基化方法的建立

Establishment of methylation specific pyrosequencing for RAR β 2 gene promoter methylation detection

在本研究中，我们首先结合传统的变性梯度凝胶电泳（DGGE）、克隆文库技术与新兴的焦磷酸测序技术比较了长期注水的孤岛油田注水井和采油井中的微生物组成。

Using denaturing gradient gel electrophoresis ( DGGE ), 16S rRNA gene clone library and pyrosequencing , we compared the microbial communities of injection well with that of production well .

Sakwa说，该突破是通过使用“焦磷酸测序”（Pyrosequencing）技术实现的。该技术能并行地提供大量信息。

Sakwa says the breakthrough was achieved by using " pyro-sequencing " technology , where massive amounts of information are produced in parallel .

三个候选甲基化差异通过靶向焦磷酸测序实验进行了验证。

Three candidate methylation differences were validated by targeted pyrosequencing assays .

指数线性聚合酶链式反应法制备焦磷酸测序反应的单链模板

Preparation of Single-Stranded DNA Template for Pyrosequencing by Linear-after-the-Exponential-Polymerase Chain Reaction

PCR-焦磷酸测序技术在沙门菌食物中毒中的应用

Application of PCR-pyrosequencing technology for food poisoning of Salmonella

利用线性指数PCR-焦磷酸测序技术快速检测3种海洋弧菌

Rapid discrimination of three species of marine Vibrios by using linear-after-the-exponential PCR coupled with pyrosequencing

本课题的主要目的是研制一种基于焦磷酸测序技术的、低成本、结构简单的基因检测装置，提供一个便宜快捷的测序平台。

The objective of our research is to design a pyrosequencing-based gene detection device of low cost and simple structure and to offer a convenient sequencing platform .

现有的焦磷酸测序系统的发展主要有两种趋向：一种是针对大规模测序的高通量系统，另一种是针对床边检测的便携式的系统。

There are two main trends for pyrosequencing : one is high throughput system for large scale genome sequencing , the other is small or portable system for point of care test purpuses .

借助焦磷酸测序技术的发展，建立焦磷酸测序技术检测拉米夫定和阿德福韦酯治疗乙肝所致乙肝病毒基因耐药突变的定量检测方法，为临床乙肝耐药诊断和治疗提供依据。

With the development of pyrosequencing , establish the pyrosequencing detection method of Lamivudine and Adefovir dipivoxil treatment of hepatitis B virus resistance mutations , and to provide the basis for clinical diagnosis of hepatitis B drug resistance and treatment .

结论：用焦磷酸微测序技术进行HLADRB基因型分析具有高分辨率的优点，该方法可应用于临床器官移植的供体/受体筛查。

CONCLUSION : Pyrosequencing used in the analysis of HLA-DRB alleles has the advantage of high resolution and can be widely employed clinically in donor / recipient selection .

应用焦磷酸微测序技术行HLA-DRB基因型分析

Applying pyrosequencing to HLA-DRB genotype analysis