汉黄芩素

总之，本研究阐明汉黄芩素抗肿瘤作用的分子机制，为确定药物靶基因以及新药开发提供依据。

In conclusion , the present study sheds new light on the molecular mechanism of the anti-tumor effect of wogonin .

采用MTT法，检测汉黄芩素体外对T淋巴细胞的促增殖作用。

MTT assay was used to examine the effect of wogonin on T lymphocytes proliferation .

结果：汉黄芩素明显抑制A2780细胞增殖，抑制作用呈时间和浓度依赖性，48h的IC50为85μg/ml；

RESULTS : A2780 cell growth was significantly inhibited by wogonin . The inhibiting effect showed concentration dependent and time dependent manners with IC50 of 85 μ g / ml.

用浓度为50、100μg/ml的汉黄芩素作用A2780细胞48h，细胞出现明显的凋亡形态学改变，琼脂糖凝胶电泳出现典型的DNA梯状条带。

After treatment with 50 μ g / ml and 100 μ g / ml wogonin for 48 hours , A2780 cells showed morphological changes associated with the characters of apoptosis under fluorescent microscope . Typical DNA ladder was found using agarose gel electrophoresis .

黄芩苷，黄芩素，汉黄芩素的检测限分别为0.05,1.0和2.0ng/mL。

The limits of detection were 0.05 , 1.0 and 2.0 ng / mL for wogonin , baicalein and baicalin , respectively .

这些基因的变化都可能是汉黄芩素抗肿瘤作用的靶点。

These differentially expressed genes may be the targets of wogonin .

目的：1、化学方法合成去甲汉黄芩素，并对其进行结构鉴定。

Objective : 1 . Designed and synthesized Norepinephrine Wogonin and identified its structure . 2 .

结论：1、采用半合成的方法合成去甲汉黄芩素，合成方法简便易行，成本低，纯度高。

Synthesize Norepinephrine Wogonin and the method is simple , of low cost and high purity . 2 .

结果表明汉黄芩素和汉黄芩苷在大鼠肠道内相互转换。

The result showed that wogonin and wogonoside could convert to each other in the rat intestine individually .

通过8种抗氧化活性测定方法，评价去甲汉黄芩素的抗氧化活性。

Established eight kinds of antioxidant activity measurement methods and investigated the antioxidant activity of Norepinephrine Wogonin . 3 .

然而，对汉黄芩素在亚于诱导肿瘤细胞凋亡剂量的生物学效应却没有相关的研究。

However , little is known about the action of wogonin on tumor cells at the sub-dose of apoptosis-inducing effect .

其中黄芩苷、黄芩素和汉黄芩素平均含量分别为11.19%、0.15%和0.07%。

The average content of baicalin was 11.19 % , the baicalein was 0.15 % , and the wogonin was 0.07 % , respectively .

总的来说，我们发现一个新的肠局部循环机制，它将提高黄酮类化合物如汉黄芩素的局部生物利用度和作用时间。

In conclusion , we have discovered a novel local recycling mechanism that will significantly enhance the local bioavailability and residence time of flavonoids such as wogonin .

因此本研究选取半枝莲的主要活性成分汉黄芩素进行研究，探讨汉黄芩素是否能够用于抗胃癌的治疗及其作用机理，为进展期胃癌寻求一种新的治疗药物。

Therefore , we aimed to observe whether a main active ingredient of Scutellaria barbata , wogonin , can be used as an anti-gastric cancer drug and its molecular mechanisms .

黄芩是我国传统中药，其主要有效成分是黄酮类化合物黄芩苷、汉黄芩苷、黄芩素、汉黄芩素。

Radix Scutellariae is a traditional chinese medicine . Flavonoids baicalin , wogonoside , baicalein , wogonin are the main active components of Radix Scutellariae .

唇形科植物黄芩（ScutellariabaicalensisGeorgi.）是一种常用中药材，其主要活性成分为黄酮类化合物，包括黄芩苷、黄芩素、汉黄芩苷、汉黄芩素等。

Scutellaria baicalensis Georgi is a commonly used Chinese herbal medicines , and its main active compounds is flavonoids , including baicalin , baicalein , wogonoside , wogonin and other factors .

通过方差分析发现，不同粒度黄芩中纤维构成、黄芩甙、黄芩素和总黄酮含量差异显著（P<0.05），汉黄芩甙和汉黄芩素含量无显著性差异（P>0.05）。

It was shown that there were significant differences in concentrations of fibers , baicalin , baicalein and total flavonoids in 5 size fractions but no significant differences in concentrations of wogonoside and wogonin .

方法用HPLC法，分别以黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量为考察指标，采用静态吸附和动态吸附相结合的方法进行筛选。

METHODS By HPLC , using the contents of Baicalin , Baicalein , Wogonoside , Wogonin as the standard , the method adopts a static absorption combined with dynamic state to select .

黄芩根的主要活性成分为黄芩苷、汉黄芩苷、黄芩素、汉黄芩素等黄酮类化合物。

Flavonoids , including baicalin , baicalein , wogonoside and wogonin , are the main active ingredients of this herb .