毛细胞

听觉损伤后毛细胞再生与聋病基因治疗策略

Strategies of inner ear hair cell regeneration and gene therapy for hearing loss

卡铂造成前庭神经元的数量明显减少，其损失程度与Ⅰ型毛细胞的损失程度相接近。

The amount of ganglion cell loss was similar to the hair cell loss .

毛细胞星形细胞瘤的CT、MRI诊断分析

Diagnosis of Pilocytic Astrocytoma by CT and MRI

结论：GM耳中毒的雏鸡耳蜗毛细胞可以再生；

Conclusion : Hair cell may regenerate after GM damage .

空白对照组未见毛细胞标志物mRNA的表达。

We did not find mRNA expression of hair cell markers in the control group .

豚鼠顺铂中毒后耳蜗毛细胞的凋亡检测及Caspase-3的表达

Apoptosis and Expression of Caspase-3 in Cochlea Hair Cell after Cisplatin Toxicity

B组小鼠ABR阈值变化明显小于A组（P<0.01），外毛细胞的损害也明显减轻；

Mice in group B significantly attenuated ABR threshold shifts with out hair cells loss ( P < 0.01 ) .

豚鼠Ⅱ型前庭毛细胞ACh-敏感性BK电流

ACh-sensitive BK current in guinea pig type ⅱ vestibular hair cells

TUNEL与碘化丙啶双染检测耳蜗毛细胞的损伤

Identification of damaged hair cells in the cochlea by double staining with TUNEL and propidium iodide

E组与A组之间无显著性差异，但E组内、外毛细胞的存活率又明显高于C、D两组（P<0.01）。

Group E and A have no difference , but E is higher than C and D , the difference between them was statistically significant ( P < 0.01 ) .

GM＋DMSO组外毛细胞受损程度较GM组明显为轻。

The structural damage of outer hair cells was severer in the GM group than that in the GM plus DMSO group .

EGF组毛细胞再生活跃，PCNA阳性细胞核明显增多。

In EGF group hair cell regenerated were active , and the number of PCNA positive cell nuclear increased obviously .

目的应用TUNEL与PI（碘化丙啶）双染法检测耳蜗毛细胞损伤。

Objective To identify damaged hair cells in the cochlea by double staining with TUNEL and propidium iodide ( PI ) .

而内毛细胞的缺失是发生在注射卡铂后72h。

However , The reduction of the inner hair cells occurred within 72 hours .

BMSCs移植到药物性聋的SD大鼠耳蜗内可以存活两周以上并可分化为具有内耳毛细胞标志物的类毛细胞。

BMSCs can be survive more than two weeks in the SD rat cochlea of drug-induced deafness and differentiate into hair cell-like cells .

川芎嗪开放外毛细胞钾通道的细胞内PKA机制

The PKA mechanism of the increasing K ~ + currents of outer hair cells by TMP

结论NGF和强化铁营养能防止大鼠受损的毛细胞进一步变性和坏死，两者联合应用有协同作用，效果更明显。

Conclusions NGF associated with fortified iron nutritional diet can obviously protect cochlear hair cell against blast exposure by decreasing degeneration and necrosis .

结论本实验证实了CBA／J小鼠耳蜗毛细胞存在的自然退化现象，并有一定的规律。

Conclusion The present study indicates that there is a regular natural missing of OHC and IHC in the cochlea of CBA / J mice .

CCGP能保护耳蜗毛细胞SDH的活性，减轻了毛细胞的损伤。

It protected the activities of mitochondrial succinate dehydrogenase in the cochlear hair cells .

KM用药一周即可豚鼠耳蜗内、外毛细胞的损伤。

KM ( 400mg / kg / d ) can impair both outer and inner hair cells in guinea pig 's cochlea , by im , after 7 days .

将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥，GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。

This promoter is shown to direct the specific expression of the reporter gene , B-glucuronidase ( GUS ), in trichomes of Arabidopsis .

孵化6d，毛细胞分化出来；

6d , hair cells diffeaentiate ;

耳蜗各回内外毛细胞均呈NOS阳性反应，内毛细胞区较外毛细胞区染色深；

Both internal and external hairy cells of cochlear nucleus show NOS positive reaction . The stain of internal hairy cells were darker than that of the external .

TUNEL阳性反应可发生于毛细胞和支持细胞，随年龄增加，阳性细胞数逐渐减少；

The differentiated hair cells and supporting cells underwent apoptosis in vestibular sensory epithelium , and TUNEL positive cells were also markedly decreased with ageing .

停药后第7天，BP听毛细胞βActinmRNA先于βactin表达至高峰。

At seventh day after treatment , β actin mRNA expression in BP increased to peak value , which preceded the peak expression of β actin in the regenerated hair cells .

B组：L-乙酰半胱氨酸（NAC）的外毛细胞平均损伤率为65%，内毛细胞平均损伤率为60%。

The mean outer hair cell ( OHC ) loss rate was 65 % in group B , and its mean inner hair cell ( IHC ) loss rate was 60 % .

目的：比较庆大霉素（GM）及其代谢产物的耳蜗毛细胞毒性，以及Ca2+、Mg2+对GM耳毒性的影响。

Objective To compare the toxicities between gentamicin and its metabolite , and study the effects of Ca ~ ( 2 + ) and Mg ~ ( 2 + ) on the toxicity gentamicin .

结果：采用这种方法培养的外毛细胞，在存活的20h内，其细胞形态和结构无明显改变。

Results : The structure and morphology of outer hair cells remained unchanged during 20 hours .

结论：1.新生SD大鼠耳蜗内存在一种具有高增殖能力的细胞，可以分化为具有纤毛样结构的毛细胞、神经元、神经胶质细胞或支持细胞。

The highly Proliferative cells were present in the cochlea of newborn SD rats , which are able to differentiate into hair cells with bundle-like structure , neuron and astrocytes or supporting cells .

激活电压为-53.85±7.8mV。2A型钾通道的数量在牛蛙毛细胞间存在差异。

The activated membrane potential was - 53.85 ± 7.8 mV . ② The amount of A type potassium channels was different in various hair cells .