梅毒螺旋体

梅毒螺旋体融合双价DNA疫苗的构建及其免疫活性研究

Construction of fusion bivalent DNA vaccine of Treponema pallidum and its immune activity

荧光PCR技术检测梅毒螺旋体DNA的意义

Application value of FQ-PCR for detection of treponema pallidum DNA

免疫胶体金与酶免疫检测HIV抗体和梅毒螺旋体抗体对照实验

Controlled experiments of testing anti-TP and anti-HIV by DIGFA and EIA

目的：建立聚合酶链反应（PCR）用于快速检测梅毒螺旋体。

Objective : To establish PCR assay for rapid detection of Treponema pallidum .

目的：评估荧光定量聚合酶链反应技术检测梅毒螺旋体DNA的应用价值。

ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA .

应用双抗原夹心ELISA法筛查献血员梅毒螺旋体抗体

ELISA with Double Antigen Sandwich for Screening Speeific Serum Anti-TP Antibody in Blood Donors

方法：采用免疫ELISA法检测2557例输血前患者血清梅毒螺旋体抗体。

Methods 2557 cases were detected by using ELISA with double antigen sandwich before blood transfusion .

梅毒螺旋体IgM抗体蛋白印迹试验诊断新生儿胎传梅毒的探讨

Diagnosis of Neonatal Congenital Syphilis with Treponema pallidum IgM Western Blot

目的建立双引物聚合酶链反应（PCR）用于同时快速检测梅毒螺旋体和单纯疱疹病毒Ⅱ型。

Objective To approach double-primer PCR assay for simultaneous detection of Herpes simplex virus ⅱ( HSV-2 ) and Treponema pallidum .

梅毒螺旋体特异性IgM抗体检测的临床评价

Clinical evaluation of specific IgM treponema pallidum antibody detection

梅毒螺旋体IgM抗体对于神经梅毒的诊断意义

Diagnostic Significance of IgM Antibodies to Treponema Pallidum in Cerebrospinal Fluid for Neurosyphilis

另外，因梅毒螺旋体十分脆弱，分离和纯化过程可破坏Tp结构的完整性。

Furthermore , isolation and purification processes compromise the structural integrity of the fragile organisms .

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

51 clinical samples were detected by dark-field microscopy and a double-primer PCR , the positive rates were 15.7 % and 19.6 % , respectively .

目的评价基因重组抗原ELISA法在梅毒螺旋体抗体检测中的意义。

Objective To evaluate an enzyme-linked immunosorbent assay ( ELISA ) technique based on recombinant antigens for detection of antibodies against Treponema pallidum .

结论梅毒螺旋体（TP）进入体内后主要诱导CD11c+型DC表达，引发Th1反应，启动细胞免疫；

Conclusions Treponema pallidum induced mainly the expression of CD11c ~ ( + ) DC and initiated cell-mediated immune response .

方法：根据梅毒螺旋体47ku膜蛋白基因序列，自行设计筛选一对特异寡核苷酸引物，建立梅毒螺旋体的PCR。

Methods : According to specific sequence of Treponema pallidum gene ( tpp47 gene ), a pair of specific primers were designed and PCR assay was developed .

梅毒螺旋体外膜蛋白Gpd在Hela细胞中的表达及鉴定

Expression of Outer Membrane Protein Gpd From Treponema Pallidum in Hela Cells

目的了解HIV感染者中梅毒螺旋体（TP）在我省的感染状况，为监测性传播疾病（STD）提供科学依据。

Objective To understand the infection of TP among HIV carriers in Yunnan province , and to offer scientific bases for surveillance STD.

目的探讨梅毒螺旋体特异性IgM抗体检测在各个时期梅毒诊断中的意义。

Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods .

建立一种用蛋白质微阵列法可同时检测血清中艾滋病毒（HIV），丙型肝炎病毒（HCV）和梅毒螺旋体（TP）抗体的方法。

An optimized method has been established to simultaneously detect multiple antibodies of HIV , TP and HCV in the serum by the way of microarray .

目的探讨梅毒螺旋体（TP）感染的不同病程与宿主免疫学变化的关系。

Objective To study the relationship between the different courses of Treponema pallidum ( TP ) infection and the immunological changes of the host .

方法采用快速反应素试验进行初筛（RPR），阳性样本采用梅毒螺旋体抗体血凝试验（TPHA）进行确认。

The positive samples in RPR were diagnosed by Treponema Pallidum Hemagglutination Assay ( TPHA ) .

结论梅毒螺旋体特异性IgM抗体检测灵敏度高、特异性强，适用于未经治疗的各个时期的梅毒诊断。

Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity , and hence can be applied in the diagnosis of syphilis in various periods .

梅毒螺旋体特异性抗体明胶凝集试验（TPPA）；

Passive particle agglutination test for detection of TP AB ( TPPA );

目的了解衡阳地区梅毒螺旋体（TP）的分子亚型的分布，为梅毒螺旋体感染分子流行病学研究提供实验依据。

Objective To find out the distribution of subtypes of T. pallidum ( TP ) in Hengyang area and to provide laboratory basis for molecular epidemiological studies of TP infection .

PCR扩增梅毒螺旋体poIA基因及其在一期梅毒诊断中的应用

Amplification of the DNA Polymerase I Gene of Treponema Pallidum by PCR and its Application in Diagnosis of Primary Syphilis

结论梅毒螺旋体IgM抗体蛋白印迹试验可作为一种确诊试验应用于新生儿胎传梅毒，尤其是胎传潜伏梅毒的早期诊断。

Conclusions In view of its high specificity and sensitivity , TP-IgM-WB could be used as a confirmatory test for the diagnosis of both symptomatic and asymptomatic neonatal congenital syphilis .

用梅毒螺旋体特异性抗体19（s）－IgM＋TPHA检测方法，确诊1例出生4天新生儿患先天梅毒。

Using 19 ( s ) - IgM-TPHA test , a case with early congenital syphilis was diagnosed in a 4 day infant .

目的了解恶性肿瘤患者梅毒螺旋体（Treponemapallidum，TP）抗体假阳性率并对阳性患者进行临床分析。

Objective To investigate the rates of false-positive reactions to Treponema Pallidum ( TP ) of patients with malignant tumors and to analyze their clinical features .

分别取全血和血清进行梅毒螺旋体血凝试验（TPHA）及4种试剂盒的检测。

The 4 rapid tests and TPHA were performed by using whole blood and serum respectively .