株野

三株野毒与减毒株CTN之间的N和G基因同源性均高于与3aG、ERA株的同源性。

However , the homologies of G and N gene between wild strains and attenuated strain CTN are much high .

而7株野毒之间的差异很小，其核苷酸序列同源性为95.8%~99.7%，氨基酸同源性为96.3%~99.1%。

But the homologies among the seven field strains were very higher , their nucleotide sequence homologies and amino acid homologies were 95.8 % ~ 99.7 % , 96.3 % ~ 99.1 % respectively .

结果在B95－a细胞上分离出10株麻疹野病毒，在Vero细胞上分离病毒未获成功。

The results suggest that the 10 strains of wild virus were successfully isolated from the B 95-a cells while none in the Vero cells .

从全国七个省市的9个鸡场53只疑似患有新城疫病的鸡中，分离到28株NDV野毒株，结果说明NDV在我国普遍存在，而且呈现蔓延的趋势；

We isolated 28 NDV field strains from 53 chickens which comed from 9 poultry industry of 7 province all over country , these results indicated ND was common in our country and gene-chip was a quick , accurate and highly efficient assay .

结果所分离到的5株HAV野毒株VP12A交接区168个核苷酸（nt）的片段与IB亚型的野毒株HM175比较，核苷酸差异>7.5%；

Results Compared with those of wild virus strain HM175 ( subtype IB ), the variation rate of the 168 nucleotides at VP1 / 2A junction regions of the 5 isolated wild type HAV strains was above 7.5 % .

用核酸序列分析鉴别甲肝病毒疫苗株与野毒株

Nucleotide sequence analysis used in distinguishing vaccine strain from wild strain of HAV

广东省东莞市1株麻疹野病毒核蛋白基因分析

Analysis on nucleoprotein gene of wild measles virus isolated in Dongguan , Guangdong province

目的比较腮腺炎病毒疫苗株与野毒株的核苷酸序列差异。

Objective To compare the nucleotide differences between the vaccine and wild mumps viruses .

中国麻疹病毒疫苗株与野毒株鉴别方法的建立

The Establishment of Method for Identifying China Vaccine Strains and Wild Strains of Measles Virus

实时荧光定量法检测新城疫疫苗株和野毒株前处理方法比较

Comparison on the pre-processing method of Newcastle vaccine strain and wild strain in the inspection by real-time fluorescence quantitative PCR

用微量中和试验测定麻疹病人急性期和恢复期血清中和麻疹疫苗株和野毒株的中和抗体水平。

The neutralization antibody levels for measles vaccine virus and wild measles virus in sera of measles patients in acute phase and convalescent phase were determined by trace neutralization test .

4株猪瘟流行野毒株病毒结构蛋白E2基因的核苷酸序列分析

Analysis of E2 coding nucleotide sequence of four prevalent virulent strains of Hog cholera virus

我国近期7株猪瘟流行野毒E2基因变异研究

Study on Mutation of E2 Gene of Seven Recent Prevalent Field Strains of CSFV in China

两株中国腮腺炎野毒SH基因cDNA直接测序和克隆测序

The PCR direct sequencing and cDNA cloning sequencing of the SH gene of two wild Chinese mumps virus strains

系统发生树分析显示，Guizhou株与国内野毒株GP和LP，及日本的5个野毒株应属同一类，其中和GP株基因型最近，推测这些毒株间有更近的共同祖先。

Phylogenetic analysis indicated that Guizhou strain was clustered with GP and LP and 5 Japan strains of late 1990 ′ s , in which the GP strain was the nearest one .

方法采用固体平皿法，对云南省32株家、野两型鼠疫菌，进行了生化特性及营养需求的研究。

Methods We selected 32 strains of rat plague and wild rodent plague to determine their biochemical characters and nutrition requirement .

结论儿童麻疹免疫血清对沪-191疫苗株及GX/06/2野病毒中和作用无差异，实现麻疹消除目标应继续维持并加强儿童麻疹免疫。

Conclusions The children 's sera against both Hu-191 and GX / 06 / 2 measles virus don 't show difference in neutralizing ability . Higher measles vaccination coverage should be maintained and strengthened in children for achieving the goal of measles elimination .

一株猴泡沫病毒野毒Pol基因的cDNA的直接测序

The cDNA Nested-PCR Direct Sequencing pol Region of A Simian Foamy Virus in Rhesus Monkey

其中碳末端450个核苷酸的序列测定和分析提示，该7株病毒为麻疹野病毒H1基因型。

Sequence analysis of450bp of C-terminus of the N gene indicated that all of the7 isolates were H1 genotype .

方法抽样采集各市、县儿童血清，用ELISA试验检测IgG滴度，然后用抗体中和试验（NT）抽样检测血清针对沪-191疫苗株及GX/06/2麻疹野病毒的中和抗体滴度，并与IgG滴度比较。

Methods ELISA and NT assay were taken to detect the titer of serum measles virus IgG and neutralizing antibody , afterward search the difference in neutralizing ability of antibodies against both Hu-191 vaccine strain and GX / 06 / 2 wild virus .

对中国蓝舌病毒1株疫苗株、31株野毒株及1株南非毒株进行测序。

S10 gene sequences of1 attenuated vaccine strain and31 Chinese field isolates & 1 South Africa strain of BTV were determined .

比较分析表明，该株与国际代表株MBB株和HM175野毒株在核苷酸水平上同源性分别为98.00%和94.00%，在氨基酸水平上同源性分别为9851%和9865%。

Sequence homology comparison showed 98.00 % and 94.00 % homology at nucleotide level , and 98.51 % and 98.65 % homology at amino acid level with international strains MBB and HM 175 , respectively .

目的鉴别在接种甲肝减毒活疫苗（H2株）后23天发生的一例甲型肝炎，其病原体是疫苗株还是野毒株。

Objective To distinguish whether the etiologic agent was the vaccine strain or wild strain of HAV for vaccine Zheng who suffered from hepatitis A on day 23 after inoculation with the live attenuated hepatitis A vaccine , H2 strain .

流行野毒与C-株毒在中和抗原决定簇上有部分氨基酸存在性质差异，可能影响C-株毒对流行野毒的中和滴度。

In their neutral cluster , there are differences in amino acid property between the prevalent field viruses and C-strain cell vaccine virus , these may have effect on neutral titer of C-strain virus to the prevalent field virus .

结果该3株病毒均无HA和血球吸附活性，逆转录聚合酶链反应和其产物的基因序列分析提示，这3株病毒属于麻疹野病毒第8基因组H1基因型。

Results The results showed that these 3 strains of measles viruses had no HA and hemadsorption activity . RT-PCR and the sequence analysis of its product suggested that these 3 strains of measles viruses were belong to the H_1 genotype of eight genetic group of wild measles virus .

结果表明，9株FPV均有REV-LTR和REV-env的整合，未检测到REV-pol和REV-env，REV基因组片段整合进FPV在我国FPV疫苗株及野毒株的现象已非常普遍。

Result indicated that the integration of REV into FPV has been common in FPV vaccines strains and field strains in China . With REV-LTR and REV-env were found in FPV , but without REV-pol and REV-gag .