无鞭毛体

目的证明亚马孙利什曼原虫前鞭毛体和无鞭毛体的基因表达水平。

Objective To detect the expression level of stage-specific genes in Leishmania promastigotes and amastigotes .

结果三种不同来源无鞭毛体体外培养物光镜检查形态相似，均与纯培养获得的无鞭毛体一样。

Results Three different sources of amastigotes analyzed by light microscope showed similar morphological features .

杜氏利什曼原虫无鞭毛体蛋白N端胞外区基因（ast1）的克隆及表达

Cloning and Expression of Extracellular Region Gene Located in N-terminus of Leishmania Donovani

与无鞭毛体特异性蛋白P-8和P-4特异性单克隆抗体进行间接免疫荧光分析显示，三种不同来源的无鞭毛体均出现阳性，且浓度与部位相似。

Indirect immunofluorescent analyses employing mAbs specific to amastigote proteins P-4 and P-8 indicated positive staining for all three types of amastigotes with similar intensity and localization pattern .

结论前鞭毛体与无鞭毛体蛋白质的表达存在差异。

Conclusion The findings demonstrate the differences in protein expression profiles between promastigotes and amastigotes .

他们发现该药能在利什曼原虫生长的各个阶段杀死它，特别是在致病的“无鞭毛体”阶段。

They found that the drug kills the parasite at both stages of its growth , particularly the disease-causing'amastigote'form of the parasite .

目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。

Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay .

亚马逊利什曼原虫与硕大利什曼原虫无鞭毛体蛋白编码基因之间高度同源，在核苷酸与氨基酸残基序列水平上的同源性分别为96%和94%。

The identity and similarity of amastin sequences for leishmania amazonensis and Leishmania major Abdou are96 % and94 % , at nucleotide and amino acid sequence levels , respectively .

结论由前鞭毛体转化的无鞭毛体能高水平表达亚马孙利什曼原虫无鞭毛体P-4特异基因，可为其生物化学及免疫学研究提供无鞭毛体来源。

Conclusion Promastigote-derived amastigotes express high level of P-4-specific gene and can be used as a source of amastigotes for biochemical and immunological studies .