放射性同位素标记

采用放射性同位素标记法研究积雪草甙对体外培养的小鼠成纤维细胞DNA合成和胶原蛋白合成的影响。

To study the effects of asiaticoside to DNA and collagen synthesis of fibroblast , the method of marked radioisotopes was used .

方法：在体外模拟动脉粥样硬化（AS）形成过程中，运用放射性同位素标记底物法、逆转录聚合酶链反应、蛋白质免疫印迹法检测ACAT1mRNA和蛋白表达水平。

Methods : Formation process of atherosclerosis was simulated in vitro . ACAT_1protein and mRNA were detected by western blotting and reverse transcription polymerase chain reaction ( RT-PCR ) .

放射性同位素标记法检测脂蛋白脂肪酶活性及其应用

Determination of Lipoprotein Lipase Activity Using Radioactive Substrate Emulsion and Its Application

放射性同位素标记核酸探针的方法

Nucleic acid probe labelled with radioisotopes

是用放射性同位素标记的抗体或抗原进行免疫沉淀反应的一种方法。

DNA-mediated immunization with a gene encoding multi-epitope antigen against infection of Toxoplasma gondii in BALB / c mice ;

内标法有染料标记、放射性同位素标记、荧光色素可见标记和被动整合雷达标记等，内标法的标记保留率较高，但成本也较高。

The internal tags including dyes , radioactive isotopes , visible implant elastomer tag and passive integrated transponder have higher retention as well as high cost .

同位素示踪研究结果显示，实验中制备的放射性同位素标记的氨基葡萄糖硫酸钠可穿过扩散池内的离体实验动物皮肤，进入皮下组织。

Isotope tracer technique showed that glucosamine sulfate above can pass through the skin in vitro of test animal in a diffusion cell and osmose into subcutaneous tissue .

以放射性同位素标记的氨基葡萄糖盐酸钠为原料制备放射性同位素标记的氨基葡萄糖硫酸钠，同时观察其穿过离体实验动物皮肤的能力。

This study prepared glucosamine sulfate with glucosamine hydrochloride marked by radio isotope and observed the osmosis ability of glucosamine sulfate passing through skin in vitro of test animal .

操作上避免了使用放射性同位素标记以及层析分离3磷酸甘油酸和2磷酸乙醇酸的复杂程序，使测定过程一步完成，极大地减少了随机误差。

In practice , the complicated procedure with radiolabelling and chromatographic separation of 3_phosphoglycerate and 2_phosphoglycolate was substituted by one_step procedure without radiolabelling and chromatographic separation to decrease the random error .

在确定了突变位点的序列后，用相应的限制酶切图谱分析和用放射性同位素标记的等位基因特异性寡核苷酸探针进行斑点杂交，结果均证明测序结果的正确性。

After the sequence of the mutation site was determined , an analysis of the restriction map of the gene and dot blot hybridization with radioactive allele specific oligonucleotide probe was designed to confirm the result of DNA sequencing .

它之所以能够继放射性同位素标记、酶标记、化学发光、电化学发光后成为一种更新、更灵敏的检测方法，主要取决于它所用标记物三价稀土离子螯合物独一无二的物理及化学性质。

TRFIA has therefore become a kind of new and more sensitive measurement method after radioisotope marking , enzymatic marking , chemiluminescence , electrochemiluminescence , primarily depending on the special physics and chemistry characteristics of lanthanide trivalence ion and its chelates .

灵敏度和特异性达到放射性同位素32P标记的水平。

The sensitivity and specificity achieved the level of the probe labeled with 32 P .

放射性同位素免疫标记自动记录系统

An Automatic Recording System for RIA Labelling

研究团队采用了放射性碳同位素标记法及其它技术来对于骆驼骨架遗骸的地点进行精确地层定位。

The research team used radiocarbon dating and other techniques to precisely pin down the layers in which camel bones are found at a number of sites .

由于基于荧光、化学发光和生物发光分析的测定方法有低的检测限，且其标记物无毒性、安全、经济，可以代替放射性同位素作为标记物。

Because the methods based on fluorescence , chemiluminescence and bioluminescence have very low detects , they have the potential to replace assays that employ radioisotopes as labels .

我们报告应用随机引物和DNA聚合酶IKlenow大片段进行放射性同位素DNA探针标记，获得高比放射性DNA基因探针的方法。

We reported the method of high radiolabeled probes by random primers and Klenow fragments of E. coli DNA polymerase I.

随着荧光染料合成技术的发展，基于荧光染料的生物标记方法已逐步取代具有放射性危害的同位素标记方法，在生物技术领域中得到了重要应用。

With the development of fluorescent dye synthesize technique , the bio-label method based on the fluorescent dye is gradually replacing the isotope label method and has been widely used in the field of biotechnology .

方法：采用不同浓度α淀粉酶及全唾液作为实验性获得性膜成分，观察经放射性同位素3H-胸腺嘧啶核苷酸标记了的血链球菌对羟基磷灰石的粘附情况。

Methods : to observe the adherence of S.sanguis labelled with 3 H-TdR on hydroxyapatite covered separately by α - amylase in varying concentrations and whole saliva as experimental pellicles .

2针对放射性同位素检测法的缺点，文章提出并比较了几种常用的非放射性同位素标记方法。

Second , aim at flaws of method radioactive marker , same methods of non - radioactive marker were raised and compared .