持家基因

精子蛋白22（Spermprotein22，SP22）是一种由常染色体的持家基因编码的蛋白。

Sperm protein 22 ( sperm protein 22 , SP22 ) is encoded by housekeeping gene of euchromosome .

以持家基因GAPDH为参照指标检测消减文库的消减效率，结果发现两个文库的消减效率均高达25倍。

The subtractive efficiency of 2 ~ 5 folds was obtained in the two libraries by using the housekeeping gene encoding glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) as the reference .

多数G蛋白基因具有持家基因的特点。

Most of the G protein genes have some characteristics of housekeeping genes .

三个时期中表示子代和亲本都表达的类型（MONO）比例次之，这些共同表达的基因可能多为持家基因，为水稻生长发育所必需。

The proportion of all expressed ( MONO ) between progeny and parents is the second in three different developmental periods . These genes may be necessary for rice growth in all developmental periods .

以苦荞持家基因H3为内参基因，采用半定量RT-PCR技术检测Fls基因在苦荞花期茎、叶和花中的表达量。

Using the housekeeping gene Histone 3 ( H3 ) from F. tataricum as reference gene , semi-quantitative RT-PCR was performed to detect the expression of Fls gene in stems , leaves and flowers during florescence .

通过单个鲜胚和冷冻胚胎的RT-PCR反应产物的凝胶电泳图谱及持家基因的扩增检测，表明冻胚和鲜胚具有相同的实验结果。

The examination about the products of RT-PCR from single 4-cell fresh embryo and freeze embryo verified that these two kinds of the embryos had the same results . Silver staining method , in stead of radioisotope , was used in the study to display the specific bands .

由此可推测，持家基因S23及L35在脊椎动物的进化过程中如同奢侈基因一样，也发生了倍增。

It is clear that the housekeeping genes like S23 and L35 had undergone a large-scale duplication in the vertebrate lineage , just like luxury genes .

荧光定量PCR方法采用双标准曲线法对CIRPmRNA进行相对定量检测，先从GenBank上公布大鼠的CIRP和持家基因GAPDH的核酸序列设计两对特异性引物并合成。

CIRP mRNA relative expression quantity was detected by the double standard curve in the real time PCR method . First , two pairs of specific primers were designed on the nucleic acid sequences of Housekeeping gene GAPDH and rats CIRP , which have been published in the GenBank .