恙虫病东方体

应用PCR/SSCP分析恙虫病东方体分离株基因型的研究

Study on Gene Type of Orientia Tsutsugamushi Isolated Strains by PCR / SSCP

实时荧光定量PCR检测恙虫病东方体

Detection of Orientia tsutsugamushi by quantitative real-time PCR assay

基因芯片技术检测恙虫病东方体DNA的方法建立

Establishment of diagnostic assay for scrub typhus with microarray techniques

恙虫病东方体Sxh951株56kD蛋白的表达及其在ELISA中的应用

Expression recombinant major outer membrane protein gene ( Sxh56 ) of Orientia tsutsugamushi Shanxi and application in ELISA

鲁豫两省恙虫病东方体感染流行病学Meta分析

Meta analysis on the epidemiology of Orientia tsutsugamushi infection in Shandong and Henan province

套式PCR应用于恙虫病东方体媒介、鼠类血块及脾脏标本的实验检测研究

Study on Vector of Orientia tsutsugamushi and Specimens of Blood Clots and Spleen Collected from Artificial Infected Mice and Field Rodents by Nested Polymerase Chain Reaction

本研究首先用PCR方法扩增了恙虫病东方体Karp株的47kDa、56kDa、58kDa蛋白基因，并对这些基因片段进行了克隆和序列测定。

The genes encoding 47-kDa , 56-kDa , and 58-kDa proteins were amplified from the genomes of Ot . Karp strain by PCR , and the gene fragments were cloned and sequenced .

恙虫病东方体Karp株56kDa与47kDa外膜蛋白基因的嵌合和嵌合基因在大肠杆菌的表达

Fusion of gene encoding 56-kDa protein with 47-kDa protein gene of Orientia tsutsugamushi Karp strain and expression of the fusion gene in E.coli

研究证明，这两种外膜蛋白均存在菌体表面并具有良好的免疫原性，已成为恙虫病东方体亚单位疫苗的重要候选分子。58kDa蛋白是恙虫病东方体的热休克蛋白，属于Hsp60家族。

Both 47 - and 56-kDa proteins are considered as the major surface proteins of Ot , which has a potential importance in development of subunit vaccine against scrub typhus .

纯化的重组蛋白应用于金标免疫层析法，可直接快速检测恙虫病东方体特异性IgM和IgG。与间接免疫荧光＄IFAT比较，其检测IgG的敏感性和特异性分别为94.2%和84二%。

The immunochromatographic assay applying the purified recombinant antigen conjugated with colloidal gold as the capture reagent can detect IgM and IgG from Orientia tsutsugamushi infection in the same time , with 94.2 % in sensitivity and 84.2 % in specificity in IgG detection , in comparing with IFAT .

国内外大量研究表明，恙虫病东方体56-kD膜蛋白（全长基因2280bp）是一个型特异性抗原，约占菌体蛋白的10-15%。

A great quantity of researches domestic and abroad indicated O. tsutsugamushi 56-kD membrane protein was a type-specific antigen which accounts for 10-15 % of the overall bacterium protein .

山东地区恙虫病东方体分离株的基因分型与序列分析

Genotype identification and sequence analysis of Orientia tsutsugamushi isolated from Shandong area

汉坦病毒和恙虫病东方体复合感染的组织细胞培养研究

Study on the coinfection of Hantavirus and Orientia tsutsugamushi in tissue cell culture

恙虫病东方体56kDa抗原基因片段在不同载体的表达

Expression of truncated 56 kDa antigen gene of Orientia tsutsugamushi in different vectors

目的检测恙螨幼虫体内恙虫病东方体，分析福建恙虫病可能流行的趋势。

AimTo detect Orientia tsutsugamushi in chigger mites and analyse the epidemic trend of scrub typhus in Fujian Province .

山东地区不同季节优势恙螨自然感染恙虫病东方体的分子流行病学调查

Study on the molecular epidemiology regarding the natural infection of Orientia tsutsugamushi in 4 species of dominant chiggers collected in various seasons from the foci of Shandong province

结论福建沿海地区恙螨的恙虫病东方体的感染率高于山区，疫源地有继续扩大的趋势。

ConclusionThe infection of O tsutsugamushi in chigger mites in coastal areas is more severe than in mountain areas , and the epidemic areas have the extended trend in the province .

目的对分离自辽宁、吉林地区的6株恙虫病东方体目的基因进行分析研究，并与国际参考株进行比较。

Objective Using PCR / SSCP to analyse the gene type of the six Orientia stustugamushi ( Ot ) strains isolated from Liaoning and Jilin areas and making a comparision between the isolated strains of Ot and the international reference strains .

目的了解秋冬型恙虫病流行季节黑线姬鼠、小盾纤恙螨、病人体内恙虫病东方体（Orientiatsutsugamushi，Ot）基因型的一致性。

Objective In order to detect the gene homologies of orientia tsutsugamushi ( Ot ) isolated from scrub typhus patients , chigger mites , and rodents of Shandong province in epidemic season .