恙虫病

结果：对12例恙虫病病人的血清进行分型，均被证实为Gilliam血清型。

Results : Serological typing of 12 cases of tsutsugamushi disease were identified as Gilliam type .

方法将小黑板放置在肾综合征出血热（HFRS）及恙虫病疫区草地上，10min后取回计算螨数并鉴定螨种。

Methods Small blackboards were placed on the grassland in the endemic areas of HFRS or tsutsugamushi disease each for 10 minutes . The larvae on the blackboards were counted and their species were identified .

儿童恙虫病C反应蛋白的变化及其意义

Changes of C-reactive Protein and Their Diagnostic Significance in Chidren with Scrub Fever

基因芯片技术检测恙虫病东方体DNA的方法建立

Establishment of diagnostic assay for scrub typhus with microarray techniques

实时荧光定量PCR检测恙虫病东方体

Detection of Orientia tsutsugamushi by quantitative real-time PCR assay

用PCR技术扩增恙虫病立克次体Sta58主要抗原基因的部分片段

Amplification of a fragment of sta 58 major antigen gene of Rickettsia tsutsugamushi using polymerase chain reaction

检测到恙虫病立克次体IgG阳性25例，阳性率为5%。

There are 25 Rickettsia tsutsugamushi positive , positive rate is 5 % .

恙虫病东方体Sxh951株56kD蛋白的表达及其在ELISA中的应用

Expression recombinant major outer membrane protein gene ( Sxh56 ) of Orientia tsutsugamushi Shanxi and application in ELISA

鲁豫两省恙虫病东方体感染流行病学Meta分析

Meta analysis on the epidemiology of Orientia tsutsugamushi infection in Shandong and Henan province

196份恙虫病患者血清IgM抗体分析

Analysis of the immunoglobulin M against Rickettsia tsutsugamushi in 196 sera from patients with scrub typhus

目的：明确广东地区恙虫病立克次体（Rt）的流行株型别。

Objective : To define the type of Rt in endemic areas of tsutsugamushi disease of Guangdong .

MARS治疗重症恙虫病合并多脏器功能障碍综合征的疗效

Using the Molecular Adsorbent Recirculating System in the Treatment of Severe Tsutsugamushi Disease Complicated with Multiple Organ Dysfunction Syndrome

套式PCR应用于恙虫病东方体媒介、鼠类血块及脾脏标本的实验检测研究

Study on Vector of Orientia tsutsugamushi and Specimens of Blood Clots and Spleen Collected from Artificial Infected Mice and Field Rodents by Nested Polymerase Chain Reaction

实验性恙虫病立克次体感染或免疫鼠的ADCC活性和cAMP水平变化

Changes of ADCC and cAMP in Mice Experimentally Infected or Immunized with R. tsutsugamushi

冻干恙虫病PHA诊断试剂盒的研制

Development of Diagnostic Kit for Tsutsugamushi Disease

目的：搞清山东省费县地区秋冬型恙虫病疫源地宿主动物种类及其携带恙虫病立克次体（Rt）情况。

In order to find out the hosts of Rt in natural focus of autumn-winter type scrub typhus in Fei county , Shandong province .

斑疹伤寒、恙虫病、森林脑炎已10a以上无病例发生；

Typhus , tsutsugamushi disease and forest encephalitis have not been found for 10 years .

本文PCR技术的引物是参考恙虫病立克次体Karp株Sta58序列设计合成的一对DNA引物。

With reference to the Sta 58 major antigen gene of Rickettsia tsutsugamushi ( Karp strain ), we had designed and synthesized a pair of DNA primers ;

本文报道地里纤恙螨雄虫人工接种恙虫病立克次体后与雌虫配对培养和传代，应用幼虫叮咬小鼠分离立克次体和PCR结合核酸杂交（核酸杂交检测PCR产物）检测子代体内立克次体的结果。

This paper reports the results of the detection of Rickettsia tsutsugamushi in the offsprings of Leptotrombidium deliense which male parents were infected through inoculating by using PCR combine DNA-DNA hybridization technique and traditional technique .

建立恙虫病立克次体抗原特异性SPRIA实验方法的研究

Study on SPRIA Established for Detection the Antibody of Rickettsial Tsutsugamushi

电洗脱纯化的重组抗原应用于间接法ELISA检测恙虫病，与间接免疫荧光法IFAT比较，其敏感性和特异性分别为91.7%和76.5%。

An indirect ELISA using this recombinant protein as coating antigen showed a sensitivity of 91.7 % and a specificity of 76.5 % , compared to IFAT .

恙虫病患者血清TNF-α、IL-8和P-sel水平的检测及其临床意义

Measuring serum levels of TNF - α, IL-8 and plasma selectin in patients with scrub typhus and their clinical significance

恙虫病立克次体（Rt）分型：Gilliam型。②内陆山林型疫源地分布于浙江。

Type of Rt : Gilliam : ( 2 ) Inland mountain-forest type in Zhejiang with major reservoir host as R.

固相放射免疫（SPRIA）在恙虫病立克次体血清学检测的研究

Detection of antibody of Rickettsial tsutsugamushi by SPRIA

本文用~（61）Cr释放试验和~3H-cAMP蛋白竞争结合分析法研究了实验性恙虫病立克次体感染或免疫鼠的ADCC活性和cAMP水平变化。

Using the 51Cr ~ release cytotoxicity detecting system and 3H-cAMP protein binding assay , the ADCC activities and cAMP levels of spleen cells were studied in mice experimentally infected or immunized with R. tsutsugamushi .

结果：恙虫病组与对照组结果有显著差异（P0.005）。

Results : There are a lot of obvious differences between the tsutsugamushi disease group and the control group ( P0.005 ) .

目的了解秋冬型恙虫病流行季节黑线姬鼠、小盾纤恙螨、病人体内恙虫病东方体（Orientiatsutsugamushi，Ot）基因型的一致性。

Objective In order to detect the gene homologies of orientia tsutsugamushi ( Ot ) isolated from scrub typhus patients , chigger mites , and rodents of Shandong province in epidemic season .

本研究首先用PCR方法扩增了恙虫病东方体Karp株的47kDa、56kDa、58kDa蛋白基因，并对这些基因片段进行了克隆和序列测定。

The genes encoding 47-kDa , 56-kDa , and 58-kDa proteins were amplified from the genomes of Ot . Karp strain by PCR , and the gene fragments were cloned and sequenced .

恙虫病东方体Karp株56kDa与47kDa外膜蛋白基因的嵌合和嵌合基因在大肠杆菌的表达

Fusion of gene encoding 56-kDa protein with 47-kDa protein gene of Orientia tsutsugamushi Karp strain and expression of the fusion gene in E.coli

研究证明，这两种外膜蛋白均存在菌体表面并具有良好的免疫原性，已成为恙虫病东方体亚单位疫苗的重要候选分子。58kDa蛋白是恙虫病东方体的热休克蛋白，属于Hsp60家族。

Both 47 - and 56-kDa proteins are considered as the major surface proteins of Ot , which has a potential importance in development of subunit vaccine against scrub typhus .