弹性蛋白酶

目的PCR扩增及克隆铜绿假单胞菌的弹性蛋白酶基因。

Objective To amplify and clone the elastase coding sequence from pseudomonas aeruginosa .

球囊损伤后血管迁徙平滑肌细胞弹性蛋白酶mRNA的表达

The elastase mRNA expression of migrating medial smooth muscle cells after the damage of balloon angioplasty

C组上清中加入胰弹性蛋白酶（elastase组）；

In group C , pancreatic elastase was added ( elastase group ) .

中性粒细胞弹性蛋白酶（NE）是一种中性蛋白酶。

Neutrophil Elastase ( NE ) is a kind of neutral proteinase .

增殖细胞和迁移细胞既有血小板源性生长因子mRNA和其蛋白表达，又有弹性蛋白酶mRNA和其蛋白表达；

The proliferating cells and migrating cells not only expressed PDGF-A mRNA and PDGF-A protein , but also expressed elastase mRNA and elastase protein .

维生素A对弹性蛋白酶所致肺气肿大鼠肺组织MMP-2、MMP-9的影响

Effect of Vitamin A on MMP-2 and MMP-9 in the Lungs of Rats with Elastase-induced Emphysema

方法：采用胰弹性蛋白酶（elastase，EA）法建立兔动脉瘤动物模型。

Methods : Establish the aneurysm in rabbit by the use of elastase .

发酵培养基中添加0.40%（w／w）的大豆分离蛋白，能较好的诱导菌体产弹性蛋白酶。

According to mutagen substance resource , it showed that 0.4 % ( w / w ) soybean protein maximumly induced elastase secreting .

类胰蛋白酶和胰酶能诱导大量乳铁蛋白和IL-8的释放，而凝血酶和弹性蛋白酶却不能。

Tryptase and trypsin , but not thrombin and elastase induced significant lactoferrin and IL-8 secretion from neutrophils .

人源弹性蛋白酶2B基因的克隆及其原核、真核载体构建和表达

Cloning , Prokaryotic Expression and Construction of Eukaryotic Expression Vetor of Elastase 2B Gene in Human

结论：细胞外弹性蛋白酶浓度较IL-8浓度更能够反映牙周袋内的炎症程度。

Conclusion : The concentration of EA-sis more accurate than the concentration of IL-8 for indicating the inflammation of periodontal pocket .

慢性阻塞性肺疾病血浆白三烯C4和中性粒细胞弹性蛋白酶的表达增加

Increasing expression of plasma leukotriene C4 and neutrophil elastase protein in patients with chronic obstructive pulmonary disease

目的观察中性粒细胞弹性蛋白酶（NE）和IL-8在大鼠哮喘中的作用及地塞米松（DM）对其功能的影响。

Objective To identify the functions of neutrophil elastase ( NE ) and IL-8 , and the effect of dexamethasone in rat asthma .

目的探讨血浆中多形核嗜中性白细胞（PMN）弹性蛋白酶与脑动脉瘤发病的相关性。

Objective To investigate the relationship between cerebral aneurysms and polymorphonuclear neutrophil ( PMN ) elastase level .

两组PA-aO2及试验组弹性蛋白酶浓度在T4时间点恢复至术前水平。同组内动、静脉血之间中性粒细胞计数的差异无显著性（P值均>0.05）。

Results The neutrophil count and the granulocyte elastase levels , the alveolar-arterial oxygen tension difference raised significantly in both two groups after cpb ( P < 0.05 ) .

弹性蛋白酶基因（PAE）的克隆及在毕赤酵母中的表达

Cloning of Pseudomonas aeruginosa Elastase Gene ( PAE ) and Its Expression in Pichia pastoris

复合碳源有利于假单胞菌SWU-B（PseudomonasspSWU-B）产弹性蛋白酶；

Complex carbohydrate promoted Pseudomonas sp SWU-B to secreting elastase .

结果球囊损伤后，迁徙细胞弹性蛋白酶mRNA、蛋白表达及其活性均有明显增加，细胞周围弹性纤维的连接处数量显著减少。

Results : The expression of elastase mRNA , its protein and activity were obviously increased after balloon injury . The contact number of elastic fibers surrounding normal medial smooth muscle cells obviously decreased .

目的检测脓毒症患者血浆中性粒细胞弹性蛋白酶（NE）和基质金属蛋白酶-9（MMP-9）水平，研究它们在脓毒症中的变化特点，探讨它们的预后判断价值。

Objective To investigate the change of neutrophil elastase and matrix metalloproteinase-9 in sepsis and determine significance in the early diagnosis and prognosis of multiple organ dysfunction syndrome .

方法SD大鼠，随机分肺气肿组，经气管内一次性滴注猪胰弹性蛋白酶800U/kg；

Methods SD rats were randomly divided into two groups first . One group was emphysematous model ; emphysema was induced by single endotracheal instillation of porcine pancreatic elastase 800 U / kg ;

IL-8和细胞外弹性蛋白酶的浓度和总量在重度牙周炎组明显高于轻、中度牙周炎组（P<0.01）。

The concentration and total amount of EA-s and IL-8 in severity periodontitis patients were significantly higher than those in light , moderate samples ( P < 0.01 ) .

以血小板源性生长因子A和大鼠胰腺丝氨酸弹性蛋白酶的地高辛标记RNA探针进行原位杂交，并用抗5溴脱氧尿嘧啶抗体进行双重染色。

In situ hybridization was performed by the RNA probe of PDGF-A and rat pancreatic elastase which was labeled with digoxin , and the double immunostaining for 5-bromo-2 ' - deoxyuridine ( BrdU ) was performed .

静脉血离心后测血浆弹性蛋白酶（NE）含量，取肺组织做肺组织细胞间黏附分子-1（ICAM-1）的免疫组化染色并进行图像分析及光镜学检查。

Specimens of lung tissues were taken for optical microscopic examination and the intercellular adhesion molecule-1 ( ICAM-1 ) of lung tissues was examined with immunohistochemical technique and image analysis .

在生殖道感染指标方面，精浆中性粒细胞弹性蛋白酶（NE）可以作为筛选和诊断男性生殖道感染的客观而有效的指标。

In the infection of genital tract index , neutrophil elastase ( NE ) in seminal plasma can be an objective and effective index in screening and diagnose male genital tract infection .

方法50只SD大鼠随机分为3组，应用弹性蛋白酶灌注颈总动脉建立颈动脉梭形动脉瘤模型。A组给予氨基胍干预；

Methods Fifty adult male SD rats ( 250-300g ) were randomly divided into 3 groups . The carotid fusiform aneurysmal model was created by a catheter-directed elastase infusion into the isolated right common carotid artery segment .

背景与目的：人分泌性白细胞蛋白酶抑制剂（humansecretoryleukocyteproteaseinhibitor，hSLPI）是一种低分子量的嗜弹性蛋白酶抑制剂，为粘膜分泌的非糖基化蛋白。

Background and objective : Human secretory leukocyte protease inhibitor ( hSLPI ), is a 12 kD nonglycosylated proteinase inhibitor with a high capacity for inhibiting neutrophil elastase ( NE ) present in mucous secretions .

弹性蛋白酶活性：主动脉样本，测定实验组与对照组损伤后2、3、5d弹性蛋白酶在动脉中膜活性值。

Activity of elastase : in aortic taken , assay the activity of elastase of experiment and control group in medial SMC 2 3 5d after injury .

目的：观察留兰香油对脂多糖引起的大鼠肺气肿样改变及猪胰弹性蛋白酶引起的C57小鼠肺气肿样改变及对MMP-9的影响。

OBJECTIVE : To investigate the effect of spearmint oil on Emphysema-like changes induced by lipopolysaccharide or porcine pancreatic elastase and increase of matrix metalloproteinase-9 . METHODS : 1 .

假单胞菌SWU-M生产弹性蛋白酶发酵条件的研究

A Study on the Fermentation Conditions of Producing Elastase by Pseudomonas ssp SWU-M

第6章中，我们报道了在均相中检测人弹性蛋白酶（HNE）的荧光适配体传感探针。

( 5 ) In chapter 6 , we report a fluorescence aptamer probe for detecting human neutrophil elastase ( HNE ) in homogeneous solution .