层析柱

凝血酶经CM离子交换液相层析柱进一步纯化。

Thrombin was further purified with CM ion-exchange liquid chromatographic column .

在长径比为25的层析柱上，以正交实验确定最佳的色层分离工艺，其分离度Rf达到0.87。

By chromatographic column of length and diameter ratio 25 , the optical chromatographic technology was determined by positive cross test , and its separating degree Rf is up to 0 . 87 . The testing results were identified by the ion chromatography .

多粘菌素B琼脂糖层析柱清除体液中内毒素

Removal of endotoxin in various solution by polymyxin b-sepharose 4B affinity chromatography

抗人C1抑制物单克隆抗体亲和层析柱分离的C1抑制物性质初探

Quality Study of C_1 Inhibitor Isolating from Anti-human C_1 Inhibitor McAb Affinity Chromatography Column

利用ProteinA层析柱分离、纯化，获得纯度较高的融合抗体。

Through Protein A affinity chromatography , the fusion antibody with high purity and biological activity was obtained .

单克隆抗体亲和层析柱在测定酱油与乳腐中黄曲霉霉素B1的应用

Application of monoclonal antibody affinity column to measurement of aflatoxin b_1 in soy sauce and fermented bean curd

融和蛋白经过Ni离子亲和层析柱纯化。

The protein was purified by Ni2 + affinity column .

Y（x）是溶质在层析柱中的正态分配函数。

Y ( x ) is a regular distribution function of the solute on the chromatogram colume .

经过Ni离子亲和层析柱纯化后，得到纯度大于85%的蛋白。

The purity coefficient of protein exceeds 85 % after purification by Ni2 + affinity column . 5 .

用葡聚糖凝胶G-75层析柱制备高纯度细胞色素C

The Preparation of Highly Pure Cytochrome C by Using Sephadex G 75

用含Ca~（2+）缓冲液清洗层析柱后（洗脱物Pc为另一重要产品），该柱可反复使用。

The affinity chromatographic column could be washed with a buffer containing Ca2 + and the PC eluted product could be reused .

采用谷眺甘肽亲和层析柱纯化GST融合蛋白，经肠激酶特异性裂解释放出目的蛋白。

The GST fusion protein was purified by glutathione affinity chromatography .

将血清直接上样DSA亲和层析柱，分离肝型碱性磷酸酶强结合组分。

The sera were chromatographed directly on the DSA lectin column to isolate the strongly binding fraction .

方法常规方法免疫BALB/c小鼠制备腹水，应用G蛋白亲和层析柱对小鼠腹水样品进行分离纯化。

Methods BALB / c mice were immunized by routine technique to prepare ascites , and the ascites samples were purified using protein G affinity chromatography column .

◆通过蛋白A琼脂糖亲和层析柱制备和纯化血浆IgG，采用分光光度法对纯化的IgG进行浓度的测定。

◆ IgG are prepared and purified by protein A agarose affinity chromatography column and the concentration are detected by spectrophotometry .

进行了5′AMPQT4亲和层析柱纯化乳酸脱氢酶（LDH）的试验。

An affinity column based on 5 ' - AMP-QT_4 was applied to purify lactate dehydrogenase ( LDH ) .

ColiBL21（DE3）中表达，镍亲和层析柱纯化。

Coli BL21 ( DE3 ) . The fusion protein was purified through Nickel-affinity chromatography column .

该多糖经SephadexG&200凝胶层析柱和高压电泳分析证明是单一成份。

The polysaccharide gave a single peak on gel chromatography with Sephadex G-200 and high voltage electrophoresis .

表达的蛋白用Ni~（2+）亲和层析柱纯化后做SDS-PAGE检测，以及蛋白印迹（westernblot，Wb）和间接ELISA实验的应用研究。

The expressed Gag antigen was detected by SDS-PAGE , Western blot ( Wb ) and ELISA after purified by Ni2 + - NTA affinity chromatography .

番茄成熟果实经抽提上鸡卵类粘蛋白（OM）-Sepharose4B亲和层析柱分离纯化制得番茄凝集素。

A lectin from the edible tomato ( Lycopersicon esculentum ) was purified by hen ovomucoid-Sepharose 4B affinity chromatography .

通过NiNTA层析柱对重组蛋白进行纯化。

The recombinant protein was purified with Ni-NTA agarose bead column , and its bioactivity was determined with hyaluronan binding assay .

所得粗肽用SP-Sepharose-FF层析柱和半制备型HPLC分离纯化。

Then purified with SP-Sepharose-FF and semi-preparative HPLC .

用Ni2+-His亲和层析柱对包涵体蛋白进行纯化，纯化后的蛋白经SDS-PAGE电泳检测呈现单一条带。

A sigle band appeared for the purified protein by SDS-PAGE after the fusion proteins were purified by Ni2 + - His affinity columns .

利用Ni亲和层析柱将表达产物进行了纯化，纯化效率达95%以上，再利用分步透析进行了纯化蛋白的成功复性。

The expression product was purified by affinity chromatography with Ni-NTA agarose , and efficiency of purification was more than 95 % . The purified protein was successfully refolded by gradient dialysis .

应用有机汞亲和层析柱分离经微球菌核酸酶有限制消化的HL－60细胞核，得到有机汞层析柱亲和及不亲和的2个核小体组分。

The unbound and bound fractions of nucleosome from HL-60 cell nuclei digested with micrococcus nuclease were separated by Hg-affinity chromatographic column .

结果：用抗scFvmAb亲和层析柱纯化的重组人源性抗HBsAgFab的纯度可达95%以上，回收率可达70%～85%。

RESULTS : The purity of purified recombinant anti-HBsAg Fab was above 95 % and its recovery rate was about 75 % - 85 % .

结果显示，在薄层硅胶H为填充剂的干层析柱上，以石油醚-乙酸乙酯（15∶1）为洗脱剂，可以将大黄酚和大黄素甲醚分离。

The results indicated that they could be separated through silica gel H for thin layer chromatography as the stational phase , and petroleum aether and ethyl acetate ( 15 ∶ 1 ) as the mobile phase .

通过索氏提取气溶胶样品，抽提物经硅胶层析柱分离，使用16种多环芳烃混合标准样品绘制标准曲线，以外标法对PAHs进行定量分析，并根据所得数据浅析了多环芳烃污染来源。

Coupled with soxhlet extraction and silicagel column chromatographic separation techniques . The method of mixed standard curves were used to quantitatively determine 16 PAHs in aerosol samples .

本实验以DNA亲和层析柱为手段分离提取Ehrlich腹水癌及各种非癌疾患小鼠的血清DNA结合蛋白（DBP），并采用了SDS-聚丙烯酰胺凝胶电泳法对之进行了比较分析。

The DNA binding proteins ( DBPs ) in the serum of mice with Ehrlich ascites carcinoma or various non-malignant diseases were separated by DNA-cellulose column and analyzed by SDS-polyacrylamide gel electro-phoresis .

用自制的免疫亲和层析柱用于转基因羊乳中rhEPO的提纯，具有很好的吸附作用，回收率达70%。

The immunoaffinity chromatography column could adsorb 70 % of rhEPO in purifying the rhEPO from transgenic goat milk .