大岩桐

大岩桐组培苗移栽技术研究初报

Primary Report on Study in Transplant Technique of Tissue Culture Seedling from Sinningia Speciosa

采用间接酶联免疫吸附法（EnzymeLinkedImmunosorbentassay，简称ELISA）测定了大岩桐花瓣发育过程中4种内源激素含量的动态变化。

The changes of endogenous hormone content during petal development in Sinningia speciosa were measured by indirect ELISA ( enzyme linked immunosorbent assay ) .

用PCR法对转化再生植株进行了检测，证实NPTⅡ已经整合到大岩桐的基因组DNA中。

PCR was used to detect transgenic plants in the regenerated shoots .

农杆菌介导的CFL和MADS-box基因转化大岩桐研究

Studies on Agrobacterium Tumefaciens-mediated Sinningia Speciosa Transformation with CFL and MADS-box Genes

本研究用野生型、突变型MAPKK基因转化观赏花卉大岩桐，为进一步研究MAPKK基因的功能奠定基础，也为花卉植物的转基因提供实验系统。

The aim of this research is to establish a transgenic system for flower plants and investigate the functions of MAPKK in transgenic plants in the future .

北方大岩桐组培试管苗瓶外移栽试验

The Research of Transplanting Test Tube Gloxinia Plantlets In The Field

影响大岩桐试管块茎形成的若干因素

Fators effecting on the formation of test-tube tubers of Sinningia speciosa plantlets

大岩桐的试管生根诱导与移栽技术研究

Study on Roots Inducement in Tube and Transplant Technique of Sinningia speciosa

大岩桐开花生物学的观察及快繁技术的研究

Study on Blooming Biological Characteristics and Rapid Propagation Technique of Sinningia speciosa

大岩桐快繁体系优化研究

Study on Optimized System for Rapid Propagation in vitro of Sinningia speciosa

以叶盘为外植体，初步建立了大岩桐的遗传转化体系。

A genetic transformation system was established by using leaf discs of Sinningia speciosa .

利用大岩桐腋芽诱导分化育苗技术研究

Research of the Technique of Cultivating Seedlings Derived by Using Axillary Bud of Gloxinia

以大岩桐的叶片为外植体进行组织培养。

The tube propagation of gloxinia was studied by using the leaves as the explants .

不同基质对大岩桐移栽成活率的影响研究

The Research for the Different Matrix 's Impact on the Survival Rate of Transplanting of Gloxinia

大岩桐的组织培养

Tissue culture of gloxinia

以大岩桐幼嫩叶片为外殖体，通过0.1%氯化汞消毒后进行组织培养，适宜条件为：（1）诱导培养基为MS+BA2.0mg.L-1（以下单位同）+NAA0.2；

Using sinningia speciosa leaves as explant treated with 0.1 % mercuric chloride , the optimum conditions for tissue culture are : ( 1 ) inducing culture is MS + BA2.0 + NAA0.2 ;

同时，对大岩桐花瓣发育过程中四种内源激素的动态变化做了定量分析，为进一步研究花器官发育的激素调控机制积累科学素材。

Meanwhile , quantitative analysis on the dynamic changes of four endogenous hormones in petal development of Sinningia speciosa was done , which provide a scientific material for the further studying the mechanism of floral organ development .