多顺反子

构建多顺反子表达载体系统的新策略

New Strategy for Construction of Polycistron Expression Vector

结论：Ecs多顺反子的串联表达为小分子蛋白的体外制备提供了一种全新的思路和方法。

Conclusion : The successful expression of Ecs polycistron provided a new method for the preparation of low molecular weight protein .

多顺反子腺病毒表达载体PCA（13）/FasL-IRES-iNOS的构建和鉴定

Construction and Identification of Polycistron Adenoviral Expression Vector PCA_ ( 13 ) / FasL-IRES-iNOS

目的：构建蛇毒锯鳞蝰素（Echistatin，Ecs）多顺反子串联多拷贝基因。

Objective : To construct a polycistron tandem repeated Echistatin ( Ecs ) gene .

结论：成功构建了多顺反子腺病毒表达载体PCA（13）/FasL-IRES-iNOS，为门脉高压症的基因治疗打下了基础。

Conclusions : It is one of the crucial steps to construct successfully the polycistron adenoviral expression vector PCA_ ( 13 ) / FasL-IRES-iNOS for the gene therapy of portal hypertension .

含mIL-12基因多顺反子逆转录病毒载体构建及其在肝癌细胞中的表达

Construction of polycistronic retroviral vector containing gene of mIL-12 and the expression in murine hepatoma cells

实验结果表明，含IRES的多顺反子逆转录病毒载体能很好地在同一靶细胞中表达多个外源基因。

This demonstrated that polycistronic retroviral vector containing IRES could efficiently express multiple therapeutic genes in the same target cell .

ERIC（IRU）局限于基因组可转录区，即多顺反子操纵子基因间区域，或开放阅读框架上、下游非翻译区。

ERIC ( IRU ) is restricted to transcribed regions of the genome , either in intergenic regions of polycistronic operons or in untranslated regions upstream or downstream of open reading frames ( ORF ) .

同时，我们对此多顺反子表达系统的性质进行研究。

We also studied the properties of this polycistronic expression system .

一种小肽的多顺反子串联表达方法

A Method of Construction Polycistron Tandem Gene of Small Peptide

番茄质体多顺反子定点整合表达载体的构建及其转烟草的研究

Construction of Tomato Chloroplast Multicistron Site Integration Expression Vector and Its Transgenic Tobacco

利用甘薯质体同源片段构建多顺反子表达载体

Construction of Multicistron Expression Vector by Using Plastid Homologous Sequences of Sweet Potato

水稻多顺反子质体表达载体的构建

Construction of Oryza Sativa Chloroplast Multicistron Expression Vector

安全环保的烟草质体多顺反子定点整合表达载体的构建

Construction of The Safely and Friendly Environmental Tobacco Chloroplast Multicistron Site Integration Expression Vector

大豆叶绿体多顺反子单交换表达载体的构建

Construction of soybean chloroplast single-cross expression vectors

目的尝试水稻质体多顺反子定点整合表达载体转化到烟草质体中表达。

【 Objective 】 The rice ( Oryza sativa ) chloroplast multicistron site integration expression vector will be transformed for expression into the tobacco chloroplast .