多角体蛋白

向病毒制剂中加入纯化的多角体蛋白后作双向电泳，发现外加多角体蛋白改变原等电点面与上述31K多肽重合。

2D electrophoresis of mixtures of virus preparations and purified polyhedrin revealed that the polyhedrin artificially added changed its isoelectric point and combined with the 31K polypeptide .

密码子选用以第三个碱基为嘧啶的密码子频率最高。多角体蛋白N端有一类似信号肽结构的26个氨基酸的疏水区。

There was a signal - peptide - like hydrophobic region of 26 amino acids in the N end of polyhedrin .

多角体蛋白基因表达后，用SDS-PAGE、Western-blot和免疫荧光技术对表达产物进行了检测。结果表明：S10原核表达质粒、重组杆状病毒成功获得；

After S10 was expressed , the products were detected and analyzed by the SDS-PAGE , Western-blot and Immunofluorescence technique .

因此，本法是一种快速、特异和敏感的检测杆状病毒的方法，其敏感度为1ml含10ng提纯的病毒粒子或多角体蛋白都能被检测出来。

A 10 ng / ml of purified virus particle antigen or polyhedrin antigen could be detected .

采用引物延伸法，对ApNPV核多角体蛋白mRNA转录起始点进行了测定，确定其位于该基因调控序列12个核苷酸高保守区的nt&50位点与AcNPV相似。

The mRNA transcriptional start site was determined by primer extension , which located at nt - 50 in the high conserved 12-mer nucleotide domain , similar as AcNPV .

昆虫病毒多角体蛋白对病毒粒子的异源包装

Heterologous package of virion with polyhedrin protein from other insect baculovirus

异源多角体蛋白包装对重组家蚕核型多角体病毒感染性的影响

Infectivity of the Recombinant Bombyx mori Nuclear Polyhedrosis Virus Containing Heterogeneous Polyhedrin

肌动蛋白抑制杆状病毒多角体蛋白基因的转录与表达

Actin Inhibiting the Transcription and Expression of Baculoviral Polyhedrin

多角体蛋白仅含有一种多肽，分子量为28800道尔顿，病毒束含有至少17种结构多肽，分子量范围在10600～95500道尔顿之间。

The polyhedron exhibites one major polypeptide with a molecular weight of 28 , 800 dalton and the virus bundles showed at least 17 structural polypeptides with molecular weights ranging from 10,600 to 95,500 dalton .

外源蛋白基因在家蚕中的表达水平相对其他表达系统已很高，但仍远远低于野生型病毒的多角体蛋白在家蚕中的表达量。

Numerous useful proteins have been expressed and the expression levels were higher in silkworm-BmNPV expression vector than that of others systems . However , the quantities of foreign gene products are far lower than that of the original polyhedra .

柞蚕NPV多角体膜蛋白类似基因的克隆和序列分析

Cloning and Sequence Analysis of the Antheraea pernyi Nucleopolyhedrovirus Polyhedral Envelope Protein Gene

家蚕核型多角体病毒酪氨酸蛋白磷酸酯酶基因核苷酸序列分析

Sequence Analysis of a Protein Tyrosine Phosphatase Gene of Bombyx mori Nuclear Polyhedrosis Virus

中国棉铃虫单粒包埋核型多角体病毒多角体蛋白基因的序列分析

Nucleotide Sequence Analysis of the Polyhedrin Gene of Heliothis armigera Single Nucleocapsid Nucleopolyhedrovirus

棉铃虫核型多角体病毒多角体蛋白聚集体特性及与其它包涵体蛋白的血清学关系

Aggregate property of HaNPV polyhedrin and the serological relationship among inclusion body proteins

核多角体病毒多角体蛋白启动基因是迄今为止在真核细胞中所发现的最强启动基因之一，是理想的表达外源基因的启动子。

The polyhedrin promotor was known to be one of the most effective promoter useful in the expression of foreign genes in the insect cells .

免疫印迹分析表明，该抗血清能与感染斜纹夜蛾多粒包埋型核型多角体病毒的细胞蛋白样品发生特异性反应。

Western blot analysis indicated that the antiserum could react with the corresponding protein existed in SpltMNPV-infected S1-zsu-1 cells .