多角体

家蚕DNA对家蚕核多角体病的诱发作用

Induction of Nuclear Polyhedrosis in the Silkworm by Silkworm DNA

三种昆虫核型多角体病毒DNA的限制性内切酶酶解分析

Restriction enzyme pattern analysis of the DNAs of three nuclear polyhedrosis viruses

四种限制性内切酶对美国白蛾核型多角体病毒DNA酶解分析

Restriction endonuclease analysis of Hyphantria cunea NPV

棉铃虫核型多角体病毒mRNA的快速制备与体外翻译

Rapid Purification and in vitro Translation of Heliothis armigera Nuclear Polyhedrosis Virus MRNA

荧光定量PCR检测家蚕核型多角体病毒在其宿主体内的增殖动态

Detection of proliferation of Bombyx mori nucleopolyhedrovirus in its host by fluorescence quantitative PCR

用ELISA检测人血清中茶毛虫核型多角体病毒

Detection of Euproctis pseudoconspersa NPV in human serum by means of ELISA

基于DNA载体的RNAi抑制家蚕核型多角体病毒复制

Inhibition of Replication of BmNPV by RNAi Based on the DNA Vector

松毛虫质型多角体病毒RT-PCR检测技术的建立

Establishment of RT-PCR assay for detection of Dendrolimus cytoplasmic polyhedrosis virus

家蚕质型多角体病毒RNA聚合酶的基因克隆及鉴定

Cloning and Identification for the RNA Polymerase Gene of Bombyx mori Cytoplasmic Polyhedrosis Virus

在蚕体重用组家蚕核多角体病毒表达HCV的C及E1区基因

Expression of C and E1 gene of HCV in silkworm with recombination BmNPV

密码子选用以第三个碱基为嘧啶的密码子频率最高。多角体蛋白N端有一类似信号肽结构的26个氨基酸的疏水区。

There was a signal - peptide - like hydrophobic region of 26 amino acids in the N end of polyhedrin .

家蚕质多角体病毒中国株RNA聚合酶基因序列测定、表达及定位

Cloning 、 Expression and Location of the RNA-dependent RNA Polymerase Gene from Bombyx mori Cytoplasmic Polyhedrosis Virus

棉铃虫质型多角体病毒AB两种类型体外复制特性的比较研究

In vitro replication of two types of Helicoverpa armigera cytoplasmic polyhedrosis virus

核多角体病毒（NPV）感染的家蚕中肠DNA多聚酶的研究

Study on the DNA polymerase isolated from the midguts of NPV infected silkworm , Bombyx mori

利用RAPD技术筛选家蚕抗核型多角体病分子标记

Studies on RAPD markers for NPV resistance in silkworm ( Bombyx mori ) using RAPD method

多角体病毒琼脂免疫双扩散和ELISA检测研究

Study of a Gel Double Immunodiffusion and I-ELISA for Detection of Spodoptera litura nucleopolyhedrosis Virus and Autographa californica Nuclear Polyhedrosis Virus

柞蚕NPV多角体膜蛋白类似基因的克隆和序列分析

Cloning and Sequence Analysis of the Antheraea pernyi Nucleopolyhedrovirus Polyhedral Envelope Protein Gene

家蚕核多角体病毒载体稳定高效表达HBeAg条件及其初步应用的研究

Study of the High-Production and the Primary Application of the HBeAg Expressed with BmNPV System

斜纹夜蛾NPV多角体基因的克隆和部分测序

Clone and Partial Nucleotide Sequence of the Spodoptera litura Nuclear Polyhedrosis Virus Polyhedrin Gene

家蚕质型多角体病毒（苏州株）S10片段的cDNA克隆及序列分析

CDNA Cloning and Sequence Analysis of Bm CPV ( Strain Suzhou ) S10 Fragment

家蚕卵DNA和蛹DNA在体外经羟胺作用后，注射于正常蚕体内可诱发核多角体病，而不经过羟胺作用则无诱发能力。

Nuclear polyhedrosis can be induced when healthy silkworm were injected with DNA from silkworm eggs or pupae after treatment with hydroxylamine . DNA not treated by hydroxylamine does not bring out induction .

表达短lef-1dsRNA的转化细胞对家蚕核型多角体病毒的抗性核盘菌中dsRNA种类及其与致病力的关系

Resistance to BmNPV of Transformation Cells Expressing Short lef-1 dsRNA

马尾松毛虫质型多角体病毒（D、CPV）混剂的研究

Study on the Mixed Drugs of D. CPV

通过PCR方法，克隆了棉铃虫核多角体病毒（HaSNPV）泛素基因（Ubiquitin，Ubi）。

The ubiquitin gene of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus ( HaSNPV ) was amplified by PCR .

中国棉铃虫核多角体病毒基因组XbaI-I片段的序列分析

Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nucleopolyhedrovirus of Helicoverpa armigera

[2]构建了能够表达多角体的重组BmNPV杆状病毒表达系统。

[ 2 ] A novel BmNPV expression system which can produce the polyhedrin was constructed .

多角体蛋白基因表达后，用SDS-PAGE、Western-blot和免疫荧光技术对表达产物进行了检测。结果表明：S10原核表达质粒、重组杆状病毒成功获得；

After S10 was expressed , the products were detected and analyzed by the SDS-PAGE , Western-blot and Immunofluorescence technique .

因此，本法是一种快速、特异和敏感的检测杆状病毒的方法，其敏感度为1ml含10ng提纯的病毒粒子或多角体蛋白都能被检测出来。

A 10 ng / ml of purified virus particle antigen or polyhedrin antigen could be detected .

检测了G1代五龄蚕血淋巴的荧光素酶活性，并且从G2代开始通过家蚕核多角体病毒（NPV）攻击来筛选抗性较高的家蚕进行传代。

The luciferase activity in blood of the G 1 generation was detected , then the resistant silkworm was selected by NPV infection from G 2 generation .

家蚕蛹在复眼着色期，经4℃冷藏24小时后，可被AcNPV（苜蓿银纹夜蛾核多角体病毒）感染。

After the silkworm pupa at the compound eye darkened stage was stored in 4 ℃ for 24h , it could be infected by Ac NPV ( Autographa californica Nuclear polyhedrosis Virus ) .