基因编码区

载脂蛋白E基因编码区及其转录调控区+113G/C多态在中国正常人群中的分布

Apolipoprotein E gene polymorphisms in the protein coding region and enhancer region ( + 113G / C ) in a healthy Chinese population

IgA肾病患者核心1β3-半乳糖基转移酶基因编码区序列分析

The sequence analysis of core 1 β 3-Galactosyltransferase gene coding region in IgA nephropathy

犬瘟热病毒A株N蛋白基因编码区的克隆与序列分析

Cloning and Analysis of Gene Encoding the N Protein of Canine Distemper Virus A Strain

利用PCR方法对阳性标本进行N基因编码区核苷酸序列的扩增和测序，获得完整的N基因核苷酸序列。

The complete nucleoprotein gene sequences of positive samples were amplified and sequenced by RT-PCR . 3 .

人类T细胞受体Vβ12基因编码区的等位多态性

Allelic Polymorphum in The Ceding Region of Human T Cell Receptor V β 12 Gene

方法：收集2例斑驳病患儿及其父母的临床资料，提取其外周血DNA，采用PCR扩增c-kit基因编码区的全部外显子，DNA直接测序，明确突变位点。

Methods : Twenty-one coding exons of the c-kit gene were amplified by PCR and followed by DNA sequencing .

在分析DNA序列复杂度、预测基因编码区和非编码的DNA边界识别等问题中，以熵为基础构造的离散量度量提供了一种强有力的工具。

Entropy-based divergence measures have provided an impelling tool in evaluating sequence complexity , predicting CpG island , and detecting borders between coding and non-coding DNA regions etc.

ABO血型基因编码区全长序列的研究及初步应用

Whole Code Sequences of ABO Gene and Its Application

目的：克隆牙胚中上游刺激因子（UpstreamStimulatoryFactor，USF）基因编码区特异片段。

AIM : To subclone upstream stimulatory factor ( USF1 ) gene in mouse tooth germ .

方法以日本血吸虫成虫DNA做模板，PCR扩增ARG基因编码区的DNA序列并测序；

Methods Prepare DNA from Schistosoma japonicum adult worms , then amplify the corresponding sequences of DNA by using the same primers for amplifying CDS .

进行测定序列，该序列与甜菜SPS基因编码区的核苷酸序列其同源性可达100%。

Sequence determination , the gene has100 % homology with SPS gene nucleic acid sequence . 2 .

目的：克隆小鼠B7-2基因编码区的cDNA序列，并构建其表达载体。

Objective To clone the sequence of the cDNA of mouse B7 2 gene and construct its expression vectors .

猪与人和鼠ob基因编码区核苷酸序列的同源性分别为88.5%和84.7%；

The overall identity of ob nucleotide sequences was 88.5 % between pig and human , 84.7 % between pig and mouse .

Pib基因编码区功能的转基因初步鉴定

Initial determination of function for Pib gene ORF region by rice transformation

方法从人新鲜淋巴结组织中提取总RNA，利用RTPCR技术扩增人TALL1及其胞外区基因编码区序列，构建人TALL1及其胞外区cDNA毕赤酵母表达载体，并进行序列测定。

Methods Human TALL-1 cDNA and the TALL-1 extracellular domain cDNA was amplified with total RNA from human lymph node tissue by RT-PCR , then cloned into Pichia pastories expression vector pPIC-9K and sequenced .

但是这4种动物BDNF基因编码区编码的成熟肽段长度一致，分别为含119个氨基酸残基的多肽。

There are no introns in all four genes and the length of the deduced mature BDNF ( 119 amino acid residues ) is the same .

目的：克隆青藏高原高原鼠兔肌红蛋白（MGB）基因编码区，并分析其序列特征。

Objective : To clone and analysis the coding region of MGB of Ochotona Princeps ( pika ) .

本文采用PCR-SSCP及PCR产物直接测序等技术，对15例肺癌患者的组织标本及8例正常对照个体血细胞SOX4基因编码区进行了突变分析。

Using PCR-SSCP and PCR product direct sequencing techniques , we analysed the ORF region of SOX4 gene encoding in15 lung cancer tissues and8 normal controls .

序列比较显示：3A的C端易变，其它区的变易呈零星散在易变山羊草两个y-型高分子量谷蛋白新亚基的鉴定及基因编码区分子克隆

In addition , the variate C terminal part of 3A protein has been identified by sequence alignment with other FMDV strains . Identification and Molecular Cloning of Two Novel y-type High-molecular-weight Glutenin Subunit Genes from Aegilops Variables

GHS大鼠基因编码区的序列组成同NC大鼠相一致，但GHS鼠1795bp位点的碱基改变未见于NC鼠。

Coding-region sequences of GHS rats and NC rats intestinal VDR gene are identical , but alteration of bp 1795 in GHS rats has not been found in NC rats .

根据不同植物肌动蛋白基因编码区的保守序列设计引物后进行RT-PCR，并采用RACE技术扩增出actin基因cDNA全长序列。

The degenerate primers were designed according to the conserved sequence of gene encoding region in different plant actin , RT-PCR was carried out , and the full length cDNA of birch actin gene was amplified by RACE .

同时，给出一种加窗傅立叶变换的方法，可以识别出DNA序列中的编码区和非编码区。然后将基因编码区的隐马尔科夫模型与前向算法相结合，实现了对外显子的识别。

At the same time , the Fourier method based on sliding window is applied to identify the coding and noncoding regions in DNA sequences . Thirdly , the forward algorithm integrated with the Hidden Markov Model of coding regions is applied to predict exons in genes .

在不计算412G>A突变情况下，POAG家族发生OPTN基因编码区的突变概率为20%，而对照组中未发现。

Rate of observed mutations in POAG family is 20 % , no mutations were found in controls .

本文使用单链构象多态性（SSCP）技术，研究了TCRVβ12基因编码区的等位变异作用。

In the present report , the sensitive technique of single stranded conformational polymorphism ( SSCP ) was used to screen for allelic sequence variations in the coding region of TCR V β 12 gene .

TAFI基因编码区的单核苷酸多态性与脑梗死的相关性探讨

The Association between Single Nucleotide Polymorphisms in the Coding Region of TAFI Gene and Cerebral Infarction

本研究同时提出了水稻稻米糊化温度调控的分子遗传假说，认为基因编码区内碱基插入、缺失或替换可能是GT改变的遗传基础。

A hypothesis for molecular mechanism for GT variation of rice is presented , that is DNA insertion , deletion or base substitution in coding sequence of alk contributes to GT variation of rice .

化学合成一段针对大鼠NgR基因编码区的siRNA，用阳离子脂质体转染大鼠皮层神经元。

SiRNA directed to the complete cds of rattus norvegicus NgR mRNA was chemically synthesized and transfected into the primary cortical neurons of rat using cation liposomes transfection reagent .

测序发现co-9中CO基因编码区中有10个碱基的缺失导致了CO蛋白C末端92个氨基酸没有被合成，这其中包含一个CCT结构域。

The late-flowering phenotype was not resumed by applying exogenous gibberellin . A 10 nt fragment deletion was found in the coding region of CO gene , resulting in premature termination of CO protein and the absence of the 92 amino acids of C-terminal domain .

结果限制性核酸酶切及序列分析表明重组表达质粒包含Pokemon基因编码区，阅读框架无移位；

Results Restriction enzyme digestion and DNA sequencing suggested that the recombinant expression plasmid contained correct encoding region of Pokemon , without reading frame shift .

结论提示癌细胞mtDNA基因编码区一级结构相当稳定，而主要的核苷酸变异可能位于其mtDNA非编码区。

Conclusions These results indicate that the primary structure of gene coding region of mtDNA isolated from carcinoma cells is highly conserved . While the major variation of nucleotide is probably located in the noncoding region .