土鳖虫

土鳖虫对激素诱导骨髓间充质干细胞成脂分化的干预作用

The Intervention Role of Ground Beetle on Steroid-induced BMSCs Adipogenic Differentiation

方法将土鳖虫分为壳、头、胸、腹4部分，采用反复冻融法提取成分并进行进一步实验。

Methods To dispart the Ground beetle into four segments which were shell , head , chest and abdomen , and adopt the method of frozen and melt to do the further test .

土鳖虫组的BMSCs细胞表面有少量的微绒毛样突起。

Eupolyphaga group : there were a small amount of microvilli-like protrusions in the BMSCs surface .

传三代BMSCs分为空白对照组、激素组和土鳖虫组。

The third generation of BMSCs were randomly divided into blank group , control group and eupolyphaga group .

结论：1.土鳖虫含药血清可以抑制BMSCs成脂分化，促使BMSCs成骨分化。

Eupolyphaga containing serum can inhibit the adipogenic differentiation of BMSCs and promote osteogenic differentiation of BMSCs . 2 .

目的：建立土鳖虫药材HPCE指纹图谱鉴别方法。

Objective : To set up the fingerprint of HPCE for medicinal material Eupolyphaga Steleophaga .

土鳖虫提取液对人胃低分化腺癌细胞BGC-823的抑制作用

The Suppression Effect of Extract of Eupolyphaga on Human Gastric Low-differentia-tion Adenocarcinoma Cell Line BGC-823 in vitro

目的研究土鳖虫体外对人胃低分化腺癌BGC-823细胞抗肿瘤活性。

Objective To investigate antineoplasmic activity of extract of eupolyphaga on human gastric low-differentiation adenocarcinoma cell line BGC-823 in vitro .

碱性磷酸酶检测结果：激素组较空白对照组明显下降（P0.05），土鳖虫组较激素组明显升高，（P0.01）。

Alkaline phosphatase test result : control group was significantly lower than blank group ( P 0.05 ), Eupolyphaga group was significantly higher than the contral group , ( P0.01 ) . 4 .

结论：土鳖虫通过提高红细胞CR1活性，增加红细胞免疫粘附功能，抑制血清ACA水平，血清中锌、钙含量的升高，可作为药物生物药效探针。

Conclusion : ESW increases activity of CR 1 of red blood cells and RCIA , inhibits serum ACA-IgG and ACA-IgA . ESW enhances serum zinc calcium level , that can be a good biological pharmacodynamics .

目的：研究土鳖虫（ESW）对地塞米松（Dex）加肾上腺素（Adr）所致阴虚火旺慢性血瘀大鼠模型红细胞免疫功能和血清抗心磷脂抗体水平变化的影响。

AIM : To study the effect of Eupolyphaga Sinensis Walker ( ESW ) on red blood cell immune adherence ( RCIA ) and serum anticardiolipin antibody level in rat model of Yin-deficiency Huo-excess with chronic blood stasis .

药用土鳖虫化学成分及药理作用研究进展

Research progress in chemical constituents and pharmacological activities of earth-turtle

土鳖虫抗血栓活性部位及其性质研究

Study on the Part of Anti-thrombus and Its Property from Ground Beetle

土鳖虫质量优化前后主要成分的分析

Analysis the main component of Eupolyphaga sue Steleophaga before and after quality optimization

目的研究土鳖虫抗血栓活性部位，并对其性质进行研究。

Objective To study the part of Anti-thrombus and its property from Ground Beetle .

土鳖虫对血虚小鼠红细胞免疫功能的实验研究

Experimental study of Eupolyphaga Sinensis Walker on red blood cell immunity in mice with blood-deficiency

目的饲喂不同的饲料，对土鳖虫若虫增重的影响。

ObjectiveTo study the effect of different diets on weight of Eupolyphaga sue Steleophaga nymph .

结论：本方法为土鳖虫药材品种鉴定与质量控制提供了依据。

Conclusion : The method can be used for the quality control of Eupolyphaga Steleophaga .

可为进一步研究土鳖虫发挥药效的物质基础提供参考。

Our efforts could be a reference for further study on material base of Eupolyphaga seu Steleophaga .

结果：土鳖虫多肽明显降低血瘀家兔血黏度、抑制血小板聚集和体外血栓形成。

Results : The ESW polypeptide could reduce obviously the blood viscosity , inhibit platelet aggregation and reduce thrombosis of the model .

激素组：应用大剂量激素诱导使其向脂肪细胞分化，土鳖虫组：激素诱导的同时给予土鳖虫含药血清干预。

Large doses of steroids were used to induce adipocyte differentiation in control group ; Both eupolyphaga containing serum and steroid were given to interfer eupolyphaga groups . 4 .