嗜热菌蛋白酶

应用嗜热菌蛋白酶快速分离活性纯椭圆囊感觉上皮细胞及其意义

Isolation of Pure Living Utricular Sensory Epithelial Cell by Use of Thermolysin

硝基作为锌离子结合基团的二肽类嗜热菌蛋白酶抑制剂

The Nitro Group as the Zinc-Binding Group Dipeptide Thermolysin Inhibitors

以PhacMetOCam为起始原料，采用三种固定化酶&α糜蛋白酶/Celite545、木瓜蛋白酶/VAEpoxy、嗜热菌蛋白酶/Celite545，经过三步酶促反应得到目标三肽化合物。

This tripeptide is a fragment of cholecystokinin C-terminal octapeptide CCK-8 . Starting with Phac-Met-OCam , the target tripeptide derivative was successfully synthesized with three immobilized enzymes , α - chymotrypsin / Celite-545 , papain / VA-Epoxy and thermolysin / Celite-545 by three reaction steps in reasonable yields .

用嗜热菌蛋白酶催化合成天冬甜精并同时进行DL-苯丙氨酸甲酯的光学拆分

Simultaneous Synthesis of Aspartame and Optical Resolution of DL-Phe-OMe by Thermolysin

因此本文加入小分子物质封闭载体表面的多余基团，阻止其对固定化酶的迫害。丙氨酸、缬氨酸、色氨酸、叔戊醇等小分子都大大提高了固定化嗜热菌蛋白酶的热稳定性。

In this work , to enhance the thermal stability of immobilized enzyme , small molecule reagents such as alanine , valine , tryptophan , tert-amyl alcohol were used to quench and block excessive activated groups on support surface .