反义基因

CⅡTA反义基因体外转染对HLA-Ⅱ类抗原表达的抑制作用的研究

The Study of the Inhibition Effect of HLA - ⅱ Expression by C ⅱ TA Antisense Gene Transfection in Vitro

柿果ACC合成酶反义基因的遗传转化

Transformation of the ACC Synthase Antisense Gene in Persimmon Fruit

乙型肝炎病毒C区反义基因载体质粒的构建

Construction of the plasmid vector containing the antisense C gene of HBV

在转反义基因植株中V大量积累而Z和A的含量非常低。

V accumulated in antisense transgenic tomato plants , but Z and A content was very low .

大鼠金属蛋白酶组织抑制物-1反义基因TA载体构建及其在肾小球系膜细胞表达的实验研究

Expression of antisense TIMP-1 gene TA cloning vector in rat glomerular mesangial cells

结论：①成功地构建了含大鼠TIMP1反义基因片段的TA克隆载体；

Conclusion : ① Rat antisense TIMP-1 gene TA cloning vector was constructed successfully .

携TGFβ1正、反义基因载体对膀胱癌细胞体外生长的作用

Effect of retroviral vectors carried sense or antisense TGF β 1 on human bladder cancer cells in vitro

康乃馨ACC氧化酶反义基因遗传转化康乃馨的研究

Genetic Transformation of Carnation with Its ACC Oxidase Antisense Gene

根癌农杆菌介导ACS反义基因转化文心兰的研究

Studies on the Transformation of Antisense ACS Gene Mediated by Agrobacterium Tumefaciens in Oncidium

手术缝线携载c-myc反义基因的研究

Study on suture carrying c-myc antisense oligodeoxynucleotide

百合遗传转化体系建立及转ACC合酶反义基因的研究

Establishment of Genetic Transformation System for Lily and Transformation with Antisense ACC Synthase Gene

结论RIα反义基因片段可诱导人肺癌细胞进入终末分化至编程性细胞死亡。

CONCLUSIONS RI α antisense genes could induce lung cancer cells to terminal differentiation and apoptosis .

农杆菌介导的次郎柿ACC合成酶与ACC氧化酶反义基因遗传转化研究

Agrobacterium-mediated Genetic Transformation of Antisense ACC Synthase and ACC Oxidase in ' Jiro ' Persimmon

稳定表达细胞周期蛋白D1反义基因逆转人胃癌细胞恶性表型

Stable overexpression of cyclin D1 antisense gene reverses the transformed phenotype of human gastric cancer cells

c-jun反义基因转染与缺氧复合烧伤血清处理心肌细胞凋亡关系的研究

Relation of c-jun antisense gene transfection and cardiomyocyte apoptosis following hypoxia and burn serum treatment

结论反义基因的导入在瞬时表达时可影响细胞中靶蛋白及其他生长相关蛋白的表达，但对细胞周期和DNA合成的影响尚小。

Conclusion Transient expression of the transfected antisense c-myc molecules can change the expression of cellular target proteins and other growth-related proteins , but has little effect on cell cycle and DNA synthesis .

结果：经PCR和PCR-Southern检测证明PG反义基因已整合进樱桃核基因组中。

Results : The PCR and PCR-Southern tests showed that the anti-polygalacturonase gene was integrated into P.pseudocerasus genome .

c-jun反义基因重组体转染对缺氧复合烧伤血清刺激下大鼠心肌细胞的保护作用

Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum

AGP小亚基反义基因导入魔芋研究

Studies on Tranformation of Anti-sense Gene of AGP Small Subunit into Amorphophallus

目的构建含大鼠金属蛋白酶组织抑制物1（tissueinhibitorofmetalloproteinase1，TIMP1）反义基因片段TA克隆载体，为进一步研究TIMP1在肾组织纤维化中的作用奠定基础。

Objective To study the role of TIMP 1 in renal fibrosis , we constructed rat antisense tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) gene TA vector .

目的观察重组逆转录病毒载体－包装细胞系统介导反义基因转移表达和抗乙型肝炎病毒（HBV）的作用。

Objective To observe the transfer and expression of HBV antisense genes and their anti HBV effects in 2.2.15 cells mediated by retroviral vector packaging cell line system .

HLA-G反义基因增强免疫效应细胞杀伤活性的研究

Enhancement of Killer Activity of Immune Effector Cells Caused by HLA-G Antisense Oligodeoxynucleotides

骨桥蛋白反义基因对乳腺癌MDA-MB-231细胞黏附能力的影响

Effect of Anti-sense Osteopontin Gene on Adhesion of Breast Cancer MDA-MB-231 Cells

目的：构建EB病毒LMP1反义基因的真核表达载体。

Objective : To construct a EBV LMP1 antisense gene expressing vector for gene therapy of NPC .

抑制效率的差异可能与反义基因片段的位置、长短以及反义RNA表达质粒载体有关。

The reasons for the differ-ences in inhibition effects may be resulted from the sizes and positions of the HCV anti-sense gene fragments or the as RNA expressing vectors .

结论成功构建人Ang-2真核表达载体，为肿瘤细胞的Ang-2反义基因治疗研究创造了条件。

Conclusion We succeed in constructing human angiopoietin-2 antisense eukaryotic expression trager .

香蕉根癌农杆菌介导ACS反义基因遗传转化及ACS基因克隆

Studies on the Transformation of Antisense ACS Gene Mediated by Agrobacterium Tumefaciens and Cloning of ACS Gene from the Leaves of Banana

Patatin、Wun1启动子驱动的PPO反义基因植物表达载体的构建

Construction of Antisense PPO Gene Plant Expression Vector Controlled by Patatin or Wound-inducible Promoter

Northern杂交分析表明，该反义基因在转基因植株中能正常转录并导致内源classⅠpatatinmRNA含量的下降。

Northern hybridization analysis showed that the antisense gene was transcribed normally in the transgenic potato plants and resulted in a reduction of endogenous class I patatin mRNA .

结论突变特异性K-RAS反义基因经基因枪有效转导后，可用于胰腺癌的治疗。

Conclusion Gene gun transduction of K-RAS Antisense gene is a potential method for treatment of pancreatic carcinoma .