原始生殖细胞

采用石蜡切片的方法对中国林蛙原始生殖细胞（primordialgermcells，PGCs）的发生及迁移路径进行研究，结果表明：①中国林蛙原始生殖细胞发生于卵黄囊内胚层；

The cytogenesis and migratory path of Rana chensinensis PGCs ( primordial germ cells ) was studied with paraffin section . Result is :① PGCs originated from the endoderm of the yolk-sac .

本研究结果初步表明：黄鳝的雄性生殖细胞可能起源于雌性阶段卵巢被膜中的原始生殖细胞。

The results suggest : The male germ cells of Monopterus albus may derive from the PGCs in the membrane of ovary .

从原始生殖细胞分离昆明小鼠EG细胞及其传代培养

Isolation of Kunming Mice Embryonic Germ Cells Derived From Primordial Germ Cells and Their Passage Culture

自原始生殖细胞分离克隆小鼠ES细胞研究初报

A preliminary study on the isolation and cloning mouse embryonic stem cells derived from primordial germ cells

大分子BAC基因打靶载体转染鸡胚原始生殖细胞

Macromolecule BAC targeting vector for transfection of chicken embryo primordial germ cells

转化生长因子β（TGFβ）超家族作为一组作用广泛的细胞因子对原始生殖细胞（PGC）及生殖母细胞的发育过程也具有调节作用。

Transforming growth factor β ( TGF β) superfamily can regulate the development of primordial germ cell ( PGC ) and gonocyte .

出生后第10d，原始生殖细胞出现一个增殖高峰；

Primordial germ cells reached a peak of proliferation at the 10th postnatal day .

统计46例山羊胎儿分离培养原始生殖细胞情况，胎龄在25~38d的胎儿原代培养时都可获得大量的细胞集落，适合做EG细胞的分离培养，最高传至6代。

The primordial germ cells were isolated from 46 goat fetuses embryonic age from 25 to 38 days , there were so many colonies in primary culture .

原始生殖细胞（PGC）是成年性腺生殖细胞的始祖细胞。

Primordial germ cells ( PGC ) are the precursors of the germ cells in the adult gonads .

原始生殖细胞（primordialgermcells，PGCs）是多细胞生物体中生殖细胞的祖先，同时也是研究细胞迁移的一个重要的模型。

Primordial germ cells ( PGCs ) are the progenitors of reproductive cells in metazoans and are an important model for the study of cell migration in vivo .

胚胎干细胞ES是从早期胚胎或原始生殖细胞中分离出来的能够在体外进行传代培养，而不分化的全能性细胞。

Embryo stem cells ( ESCs ) are a kind of omnipotent cells separated from early embryo or primordial germ cells that can be cultured in vitro for many generations without differentiation .

FANCL在原始生殖细胞的形成和范可尼贫血中的功能研究

Functions of FANCL in Primordial Germ Cell Formation and Fanconi Anemia

转基因动物乳腺生物反应器研究进展鸡的原始生殖细胞（primordialgermcells，PGCs）是鸡各级生殖母细胞和成熟配子共同的祖先，最终发育分化成卵子或者精子。

Progress in Mammary Gland Bioreactor of Transgenic Animals Chicken primordial germ cells ( PGCs ) were the ancestor of all level germ cell and transgenic animals have become important tools for biological research .

采用连续切片法进行鸡胚胎显微观察，研究鸡原始生殖细胞（PGCs）迁移特性及其与性腺发生、发育的关系。

The relationship between PGCs migration and gonad development of chick embryo which was incubated for 3 hours ~ 7 days were studied .

以14￣15期鸡胚血液为材料，采用Ficoll密度梯度离心方法，提取鸡胚胎原始生殖细胞（primordialgermcells，PGCs），在无基质细胞和基质细胞上分别进行体外培养。

Primordial germ cells ( PGCs ) were isolated from the blood of stage 14-15 chick embryos by using Ficoll density centrifugation . After isolated , primordial germ cells were cultured with or without stroma cells .

为了更深入的认识人EG细胞，我们进行了以下研究：1.进一步研究了人胚胎原始生殖细胞的发生、迁移及表型特征。

In order to recognize further human EG cells , we have researches as followed : ( 1 ) further research the germination , migration and surface marker of human embryonic germ cells which are the origin of human EG cells .

通过微注射法将鸡原始生殖细胞（PGCs）注入到鸭的胚盘下腔中，用鸡W染色体DNA探针通过原位杂交的方法对供体PGCs在嵌合体胚胎性腺外的发育作了研究。

Chicken PGCs from a line of White Leghorns were microinjected into Maya duck blastoderms at stage ⅹ . Extragonadal development of donor PGCs in chicken duck chimeric embryos were studied by in situ hybridization with W chromosome specific DNA probes .

D/F12-EB基础培养基较其它培养基更能支持生殖细胞的体外分化，Wnt3a和BMP4能有效促进原始生殖细胞的形成。

Compared with other medium , D / F12-EB base medium is more likely to support the differentiation of germ cell in vitro .

胚胎生殖（EG）细胞是来源于胚胎原始生殖细胞（PGCs）的多潜能干细胞。

Embryonic germ ( EG ) cells are pluripotent cells derived from primordial germ cells ( PGCs ) of gonads , gonadal ridges and mesenteries , analogies of fetuses , with the ability to undergo both highly self-renewal and multiple differentiation .

人胚胎干细胞（ESC）起源于囊胚的内细胞团，而人胚胎生殖细胞（EGC）由发育后期的原始生殖细胞（PGC）发育而来。

ESCs are derived from the inner cell mass of pre-implantation blastocysts , whereas embryonic germ cells ( EGCs ) are derived from the primordial germ cells ( PGCs ) of embryos in later stages of development .

目的研究鸡胚胎原始生殖细胞（EPGCs）对不同冷冻体系的适合性。

Objective This paper focused on comparing the effectiveness of different cryoprotectants for chicken embryo primordial germ cells ( EPGCs ) .

通过组织学方法观察江黄颡鱼原始生殖细胞（PGCs）迁移、生殖嵴生成和性腺分化，并且探讨在不同温度培育下性腺分化的差异。

The migration of the primordial germ cells ( PGCs ), formation of the genital ridge , gonad sex differentiation , and their variations under different water temperatures were investigated using histological methods in the teleost , Pseudobagrus vachelli .

目的：对不同发育阶段的人胚胎原始生殖细胞（PGC）进行定位并比较其体外培养的差异。FANCL在原始生殖细胞的形成和范可尼贫血中的功能研究

Objective : To study the localization and in vitro culture of human embryonic primordial germ cell ( PGC ) during different stages of embryogenesis . Functions of FANCL in Primordial Germ Cell Formation and Fanconi Anemia

ESCs来源于哺乳动物的囊胚内细胞团和原始生殖细胞，具有全能分化和无限增殖两大特性。

Embryonic stem cells ( ESCs ) were isolated from inner cell mass of mammalian blastocysts and primordial germ cells , which had two major characteristics , one is unlimited proliferation , another is the capcity of differentiation into all cell types .

胚胎干细胞（ESC）建系取材包括桑椹胚的卵裂球、囊胚的内细胞团（ICM）、上胚层细胞和原始生殖细胞（PGCs），甚至从新生鼠睾丸细胞也分离得到ES样细胞。

The inner cell mass ( ICM ), blastomeres , epiblasts and primordial germ cells ( PGCs ) are usually used as primary materials for the establishment of embryonic stem cell ( ESC ) lines . ES-like cells have even been isolated from neonatal mouse testis .

Germcell-less（GCL）是与原始生殖细胞发生相关的重要因子，果蝇、斑马鱼、青鳉鱼和小鼠的GCL蛋白都含有一个进化上保守的BTB/POZ结构域。

Germ cell-less ( GCL ) plays an essential role in the formation of primordial germ cells . An evolutionally conserved domain , BTB / POZ domain , was found in the GCL homologue proteins of Drosophila , zebrafish , medaka and mouse .

本文对96例卵巢早衰（POF）患者进行了病因探讨，经有关检查提示，家族倾向、染色体异常和卵巢发育不全导致原始生殖细胞数少或缺乏可能为POF的主要原因；

The etiological cause of 96 patients with premature ovarian failure was analyzed . The result suggested that the main cause of premature ovarian failure might be the deficiency or decrease in the number of primordial germ cells , which results from genetic disposition , chromosomal abnormalities and ovarian dysgenesis .

胚胎期FANCL与小鼠原始生殖细胞增殖密切相关，成年睾丸中FANCL与几个生殖细胞特异性蛋白形成一个睾丸特异网络，可能参与影响精子的生成。

FANCL is involved in the proliferation of primordial germ cells ( PGC ) in early embryonic stages , and may play a role in the development of germ cells by forming a novel testis-specific network with testis-specific proteins in the adult testis .

鸡原始生殖细胞迁移和聚集规律的研究

Migration and accumulation of primordial germ cells in the chick embryo

原始生殖细胞的人类胚胎干细胞克隆

Embryonic Stem Cell like Colonies from Primordial Germ Cells in Human