单克隆抗体技术

目的：利用单克隆抗体技术检测闭角型青光眼（ACG）患者外周血T细胞亚群，以了解ACG患者机体的整体免疫平衡状态。

· AIM : To understand the whole immunologic balance state of the body of angle-closure glaucoma ( ACG ) patient by testing T cell subset in the peripheral blood of ACG patient with monoclonal antibody technique .

单克隆抗体技术皮肤浆细胞增多症1例

Monoclonal Antibody Technique Cutaneous plasmacytosis : A case report

本文应用单克隆抗体技术，检测了30例心血管疾病病人的外周血B细胞、T细胞及其亚群。

The peripheral blood B and T lymphocyte subsets of 30 patients with primary hypertension and coronary heart disease were determined by utilizing McAbs .

单克隆抗体技术分泌抗细粒棘球蚴囊液抗原McAb杂交瘤细胞株的建立

Establishment of hybridoma cell line secreting monoclonal antibodies ( McAb ) against hydatid cyst fluid antigen

乳过氧化物酶法制备~（125）Ⅰ标记膀胱癌（L4B4）单克隆抗体技术

Preparation of ~ ( 125 ) i-labeled monoclonal antibody of bladder neoplasm using lactoperoxidase

单克隆抗体技术（monoclonalantibodytechnique）又称杂交瘤技术，是20世纪70至80年代诞生的生物高新技术。

The monoclonal antibody technology ( monoclonal antibody technique ) is also called the hybridoma technology , and it is a high and new biological technology which was borned in the 70s and 80s of 20th century .

方法分别应用放射免疫分析和单克隆抗体技术对31例癫痫患者进行了血清IL-2、IL-6、TNF-α含量及外周血B细胞及T淋巴细胞亚群水平的测定，并与35例正常人作比较。

Methods Serum IL-2 , IL-6 and TNF - α levels were determined with RIA and peripheral lymphocyte examination were performed with mono-clonal antibody technic in 31 patients with epilepsy and 35 controls .

流式细胞仪（FlowCytoMeter，FCM）是一项集激光技术、电子物理技术、光电测量技术、计算机技术以及细胞荧光化学技术、单克隆抗体技术为一体的新型高科技仪器。

Flow cytometer ( FCM ) is a collection of electronic and physical technology , photoelectric measuring laser technology , computer technology and the cell fluorescence chemical technology , monoclonal antibody technology as a new type of high-tech instruments .

采用一步法单克隆抗体技术和微量序列特异性引物聚合酶链反应（Micro-PCR-SSP）技术进行HLAⅠ类和Ⅱ类分型。

Donor and recipients HLA class ⅰ and ⅱ typing was performed by one-step monoclonal antibody and micro polymerase chain reaction with sequence-specific primers ( Micro-PCR-SSP ) .

通过单克隆抗体技术对IBV抗原表位的研究，不但可以深入了解IBV的抗原结构，而且便于设计科学合理的表位疫苗和诊断试剂，对IB的诊治具有重要的意义。

IBV epitope studies by monoclonal antibody technology , not only help to understand the IBV antigenic structure in depth , but also facilitate to design scientific and rational epitope vaccines and diagnostic reagents .

在分析二氟沙星（Difloxacin，DIF）分子结构和免疫原性基础上，运用单克隆抗体技术制备其单抗，并组装了竞争ELISA试剂盒和胶体金试纸。

In the study , Based on the analysis of molecular structure and immunogenicity of Difloxacin , the technology of monoclonal antibodies production was applied to assemble rapid ELISA kit and strip for DIF residual detection .

方法应用流式细胞仪（FCM），荧光素标记单克隆抗体技术测定68例2型糖尿病患者（血管病变35例，无血管病变33例）及31例正常人CD62P、CD63的阳性表达率。

Methods 68 type 2 diabetes mellitus ( 35 cases with angiopathy , 33 cases without angiopathy ) and 31 normal individuals entered this study . The expression level of CD62P and CD63 were measured by flow cytometric immunoflurescence .

方法：分别应用单克隆抗体技术和酶联吸附试验检测31例白血病患者外周血T淋巴细胞亚群和sIL2R水平，并以35名正常人作对照。

Methods : Serum levels of sIL 2R and T lymphocyte subgroup in 31 leukemia patients and 35 healthy persons as control were determined by Elisa and monoclone of antibody technique .

单克隆抗体技术的发展&基因工程抗体

Development of monoclonal antibody technology & genetic engineering antibody

应用单克隆抗体技术分析鼻疽菌与类鼻疽菌表面抗原的初步研究

A preliminary analysis of surface antigens between PS. mallei and PS. pseudomallei using monoclonal antibody

单克隆抗体技术在植物中的研究应用

Application of Monoclonal Antibody Technology on Plant

兔单克隆抗体技术应用于转基因食品蛋白的检测国内外未曾有相关报道。

There is no report involved the application of rabbit McAb in detection of GMF proteins at home and abroad .

方法：应用T淋巴细胞单克隆抗体技术对部分胃溃疡并真菌感染患者及对照组外周血和胃粘膜T细胞亚群进行检测。

Methods : The T lymphocyte subset in peripheral blood and gastric mucosa of some gastric ulcer patients with fungus infection were measured with the technique of T lymphocyte monoclonal antibody .

本文综述了国内外单克隆抗体技术在小麦贮藏蛋白研究及其遗传改良中的应用进展，并展望其应用前景。

This article has reviewed recent progress in the application of monoclonal antibody technology in the studies of wheat endosperm storage proteins and genetic breeding . The prospect of its application in these studies is also discussed .

单克隆抗体技术对现代生命科学研究的发展起到巨大的作用，作者着重介绍了畜禽单克隆抗体研制现状及其在畜禽疫病诊断中的应用，并对其面临的问题进行了讨论。

Monoclonal antibody techniques are very meaningful in modern life science research . This review mainly introduces the recent status of monoclonal antibody and the application in diagnosis of animal diseases , and gives a discussion of the problems in monoclonal antibody researches .

采用单克隆抗体免疫技术，检测36例类风湿性关节炎（RA）患者外周血淋巴细胞亚群。

By using monoclone antibody immune technique , the lymphocyte subgroups in the peripheral blood of 33 healthy adults and 36 rheumatoid arthritis ( RA ) Patients were examined .

方法：利用流式细胞仪和单克隆荧光抗体技术测定31例BD患者和27例健康对照者外周血中CD4+CD25+调节性T细胞在CD3+CD4+细胞中的比例。

Methods : The proportion of peripheral blood CD4 + CD25 + Tr was determined by flow cytometry in 31 patients with BD and 27 healthy controls .

复合免疫制备3种有机磷农药单克隆抗体的技术研究

Preparation of organophosphorus pesticide monoclonal antibodies against multiple antigens

二者的结构多肽NP蛋白和F蛋白用特异性单克隆抗体免疫转印技术分析，未显示差异。

The viral NP and F proteins revealed the same patterns by Western Blotting with specific monoclonal antibodies .

采用单克隆抗体免疫荧光技术，流式细胞术检测，观察了1.5GyX射线单次全身照射（剂量率0.286Gy／min）对小鼠胸腺细胞CD3分子表达的影响，及胸腺细胞亚群和细胞周期的变化。

The effect of whole body irradiation on CD3 expression of thymocytes was investigated in Kunming mice which were irradiated with 15 Gy X-rays at the dose rate of 0.286 Gy / min.

应用单克隆抗体免疫荧光技术检测马铃薯青枯病潜伏侵染的初步研究

Monoclonal antibodies used to detect latent bacterial wilt infections by an indirect fluorescent antibody staining procedure in potatoes

结论建立了通过单次细胞融合制备针对复合抗原的单克隆抗体制备技术，使单克隆抗体的产出率显著提高，制备周期明显缩短，为建立规模化单克隆抗体制备平台奠定基础。

Conclusion The mAbs against multiple antigens have been obtained by single cell-fusion , which increases the production of mAbs and reduces the time of preparation .

流式细胞术（FCM）单克隆抗体直接免疫标记技术检测白血病细胞分化抗原。

Immunohistochemistry ( Flow Cytometry , FCM ) .

方法：本实验应用单克隆抗体桥联酶标技术，对OLP患者外周血中T淋巴细胞亚群及B淋巴细胞亚群进行初步研究。

Methods : To study the subgroup of T lymphocyte and the subgroup of B lymphocyte existing in OLP patients ' peripher-blood by using the technique of APAAP .

方法用PCR-SSP以及单克隆抗体血清学分型技术对HLA-A、B分型并比较。

Methods DNA samples and were obtained from 34 clinical patients , and serological typing with monoclonal antibody ( mAb ) and HLA-A and , B antigen genotyping with PCR-SSP were performed .