分离胶

分离胶采血管制备血清对血糖、补体C3和NSE测定结果及稳定性的影响

Influence of serum preparation with separating gel vacuum tubes on the determination results and stability of blood glucose , complement C3 and neuron specific enolase

目的：对血液分离胶的制备进行研究。

Objective : To study the preparation of blood separating gel .

3对蛋白质的胶上酶切方法进行了考察，通过对SDS一PAGE分离胶上蛋白质进行还原、烷基化等处理，改变酶切条件，得到一种适用于sDS一PAGE分离蛋白质的酶切方法。

Iii. An optimal in-gel digestion procedure for SDS-PAGE separated proteins was acquired via additional reduction and alkylation of the protein in gel slice .

静脉采血（EDTA抗凝1管，分离胶促凝1管）。

Intravenous blood sample was collected ( with one tube for EDTA anticoagulation and one tube for separation gel coagulation ) .

通过文献检索和比较，选择3%浓缩胶和10%的分离胶作为电泳胶板，以低分子量标准蛋白为marker电泳。

3 % of concentration gel and 10 % of separation gel was chose as electrophoresis plastic through literature search and comparison , low-molecular-weight standard protein as marker for electrophoresis .

结果表明：6%的分离胶对PSI具有较好的分离特性；

The results showed that 6 % was the best concentration of separation gel for PSI complex separation .

结果表明，分离胶浓度大小对大豆贮藏蛋白（7S和11S组分）亚基电泳图谱分辨率的影响非常明显。

The results showed that the resolving effect of soybean major storage protein subunits could be greatly affected by the concentration change of resolving gel .

如需获得较好的7S和11组分亚基综合分辨效果，以分离胶浓度为12%的凝胶系统为佳。

Furthermore , the 12 % resolving gel concentration should be the best choice to obtain the good resolving effect of IIS and 7S fraction subunits .

且可以用于试管薯、微型薯生产，而具有一定的生产意义。2.得马铃薯蛋白质SDS-PAGE的优化体系：分离胶为12%，凝胶厚度为0.75mm，加样量为10ul。

System of SDS-PAGE was optimized , conditions being running gel 12 % , gel thickness 0.75 mm , and loading amount 10 ul .

速效剂法酵解血糖6h内差异无显著性（P>0.05），分离胶法可稳定96h以上，推测血糖可长期稳定。

The difference was not significant in quick anticoagulant method > 6 h ( P > 0.05 ) . The glucose was stable > 96 h in inert gel tube and might be stable in quite a long time .

磷酸盐分离胶磷矿和白云石的作用机理

The Mechanism of Separation of Francolite and Dolomite with Phosphates as Depressants

血液分离胶的制备研究

A Study on the Preparation of Blood Separating Gel

血清分离胶对生化指标测定的临床应用研究

Clinical application of biochemical assay with serum separating medium

可以采用聚丙烯酰胺凝胶电泳，分离胶浓度为7.5%，pH8.9；

Polyacrylamide gel electrophoresis were made using 7.5 % separation gel ( pH 8.9 );

分离胶真空分离器血氨浓度在4℃8小时内几乎不变。

The stability of blood ammonia concentration with separate gel might be remanied in4 ℃ for8hours .

目的观察用分离胶血液真空采集管采集的血糖标本，按其浓度与放置时间的不同进行分组，观察血糖浓度的变化情况，以评价血液葡萄糖在分离胶血液真空采血管中的变化趋势。

Objective To observe the changes of concentration of blood sugar in separation gel vacuum collective tube .

分离胶浓度不同，酶谱有差异，7.5%和8.0%的凝胶浓度是最佳选择。

Esterase Isozyme patterns was different in the different treatments , the treatments with 7.5 % and 8.0 % were the best method .

结论国产分离胶对于稳定血清中的生化成分，具有良好的效果，可在临床推广应用。

Conclusions The home-made separate gel has good effect on stabilizing the biochemical components in serum and can be popularized in clinical application .

结论血清分离胶可用于对非抗凝血标本的血清分离，可以保持被分离血清各成份的性状，并且还可以保持血清各成份的稳定性，在临床检验中有很广泛的用途。

Conclusions The serum separating medium used for non-anticoagulative serum can keep the stability of all kinds of components in serum , It may be widely used in clinical examinations .

方法采用惰性分离胶试管收集样品，与血糖速效剂法进行对照，设置不同时间配对测定血糖。

Me thods The blood sample was collected with separator tube containing inert gel and compared with the quick anticoagulant method as control by matched pair in glucose determination at different time .

不同品种间大豆分离蛋白胶凝能力及评价方法研究

Gelation capacity and its evaluation methods of soybean protein isolates from different soybean varieties

泡沫分离法处理胶团强化超滤渗透液的机理与应用研究

Mechanism and Application Research of the Permeate in Micellar-enhanced Ultrafiltration by Using Foam Fractionation

本文叙述了半湿法分离提取植物胶的生产方法、工艺特点以及原料要求。

The producing method , technological traits and requirements of raw material about the dipping way on extraction galactomannan have been discussed in this article .

首先使用适当溶剂，通过分级沉淀分离粘鼠胶的各组分，然后用傅立叶变换红外光谱仪、核磁共振波谱仪对分离后的组分进行结构鉴定。

At first , the components of Rat Trapping were separated by stepwise precipitation using suitable solvents . Then , the chemical structure of these components were characterized by Fourier Transformation infared spectrometer and Nuclear Magnetic Resonance spectrometer .

琼脂糖凝胶电泳分离PCR产物，胶回收试剂盒回收分离目的基因片段。

PCR product was separated by agarose gel electrophoresis , then gel extraction kit isolated purpose gene fragments .

本文采用溶剂-沉淀法，从杜仲树叶、皮、种子分离提纯得到杜仲胶，通过偏光显微镜（PLM），红外吸收光谱（IR）等手段对杜仲胶进行了研究。

Gutta-percha was separated from leaves bark and seeds of the tree by solvent-precipitation method . It was investigated by Polarizing Microscope ( PLM ) and Infrared spectroscopy ( IR ) .

醇析法分离提纯黄原胶工艺研究

Study of Process for Separation and Purification of Xanthan Gum by Alcohol Precipitation

模切辊采用汽缸自动分离装置，减少胶垫磨损。

Die-cutting roller cylinder type auto separation unit can increase wear of rubber sleeve .

醇析法是一种有效分离提纯黄原胶的方法。

Alcohol precipitation is an efficient method for separation and purification of xanthan gum .

微生物发酵-膜分离法制备褐藻胶寡糖及其产物分析

Preparation of oligosaccharides from alginate by fermenting combined with membrane separation method and analysis of the oligomers