克隆位点

方法：通过双酶切把PScDNA全长序列接到pBlueskriptⅡKS（+）的多克隆位点上；

Methods : Link the PS cDNA full-length sequence to the multiple cloning site of pBlueskript ⅱ KS ( + ) by double restriction enzyme digest .

根据编码以上4个差异蛋白质的DNA序列，发现编码以上4个差异蛋白的基因分别位于水稻染色体4、7、8和12上的特定克隆位点，这就是与化感作用相关基因。

The genes ecoding these four differential proteins were located on the chromosome 4,7,8 , and 12 of rice .

采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。

Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers , a novel megaprimer method was performed in one tube with two steps and34 cycles .

重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。

The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers .

在CD45/SSC、CD71/CD33、CD15/CD11b散点图中M4/M5的粒细胞和单核细胞克隆位点明显不同。结论：CD14对单核细胞相关性白血病具有高特异性，敏感性不足；

Within the CD45 / SSC , CD71 / CD33 and CD15 / CD11b scatter diagram , the clone site of granulocyte and monocyte were obviously different between M4 and M5 . Conclusion : CD14 was high specificity and low sensitivity in diagnosing MLIL ;

方法用RT-PCR的方法提取全长人组织因子途径抑制因子（TFPI）基因，引入Kozak序列和亚克隆位点，将（Kozak）TFPI序列连入T载体中测序。

Methods The full length human tissue factor pathway inhibitor ( TFPI ) gene was extracted by RT-PCR . The Kozak sequence and subclone sites were led in TFPI gene . Subsequently , the ( Kozak ) TFPI sequence was connected with T vector for sequencing .

在核酸杂交中设计了噬菌体载体克隆位点互补探针，以更加准确地排除无外源基因插入的克隆；

Additionally we used the probe oligodeoxynucleotide complementary to vector clone site sequences to identify clones which were not inserted exogeneous genes .

构建了8个由不同启动子调控的、带有不同克隆位点的、分别适合于在单子叶植物和双子叶植物中表达的植物表达载体卡盒。

Eight plant expression vector cassettes were constructed , which were regulated by different promoters , contained different cloning sites and were fit for expression in monocotyledon or dicotyledon respectively .

随机挑取11个噬菌斑，用载体克隆位点两端的通用引物进行扩增，以检测所构建的文库的质量。

Then λ phage packaging reaction and library amplification were performed . Eleven plaques were randomly picked and tested using PCR method with universal primers derived from the sequence flanking the vector .

目的：为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息，应用反向PCR克隆整合位点序列。

Objective : To investigate the information of transgene integrations in mice mediated with lentiviral vectors , the inverse PCR was used to clone the sequences of integration sites .

使用inverse-PCR、Tail-PCR等方法克隆了插入位点边端序列。

Flanking sequences of the insertion sites were isolated by inverse-PCR and Tail-PCR methods .

遗传连锁作图、定位克隆、数量特性位点作图、微阵列分析及转录沉默等，是近年来常用的基因组学研究技术。

Genetic linkage mapping , positional cloning , quantitative loci traits mapping , microarrays and transcriptional silencing are genomic technologies that being widely used in recent years .

随机选取50个克隆，用位于pRAC质粒多克隆位点两侧的测序引物进行菌落PCR扩增，检测文库插入片段的分布情况。

50 clones were selected randomly and the colony PCR amplification was used to detect the distribution of library inserts with pRAC sequencing primers .