乳糖酶

而乳糖酶mRNA水平无差异。

Meanwhile , the lactase mRNA levels displayed no diversities .

min，矿质元素钠、铁和钙能促进乳糖酶的酶活力。

Na , Fe and Ca helped to promote the enzyme activity of lactase .

固定化后的乳糖酶的pH，热稳定性都较游离酶有明显提高。

Compared with free enzyme , the pH and thermal stability of immobilized lactase were increased .

乳糖酶在SD大鼠小肠中的分布情况的研究

Study on the Distribution of Lactase in the Small Intestine of SD Rats

乳糖酶、固定化乳糖酶的最适pH值分别为5.0~5.5,5.5~6.5；

The optimum pH value for immobilized galactosidase ( IGOS ) and galactosidase ( GOS ) were 5.5-6.5,5.0-5.5 , respectively .

BALB/c小鼠乳糖酶基因Lac的克隆及其cDNA探针的制备

Cloning and Preparation of cDNA Probe from BALB / c Mice

各处理组各肠段乳糖酶的活性差异不显著（P0.05）。

No significant difference was observed in lactase activity from the three segments of intestine of each treatment group ( P0.05 ) .

结论所获得的Lac基因经鉴定确为乳糖酶基因，由它编码的蛋白产物是乳糖酶。

Conclusion The Lac gene thus obtained is lactase gene authentically ;

目的研究乳糖酶活性及其mRNA在SD大鼠小肠中的分布特点。

Objective The article deals with the study on the distribution of lactase and its mRNA in the SD rat small intestine .

置人工肠液中lh，乳糖酶释放率达100%。

100 % of the enzyme was released in artificial intestinal juice within an hour .

经H2呼气试验结果判定为乳糖酶缺乏的个体，经13C乳糖2H葡萄糖负荷试验分析显示小肠粘膜仍存在一定乳糖酶活性。

The 13C-lactose / 2H-glucose challenged test showed there was still residual lactase activity in small intestine .

目的制备表面功能化磁性微球用以分离血管内皮生长因子（VEGF）核酸和固定化乳糖酶。

Objective To Prepare surface functional magnetic microspheres for the separation of vascular endothelial growth factor ( VEGF ) nucleic acid and lactase enzyme immobilization .

来源于芽孢杆菌（Bacilluscirculans）的乳糖酶在毕赤酵母中的高效表达

Overexpression of lactose from Bacillus circulans in Pichia pastoris

饲喂牛乳补加胰岛素组仔猪小肠粘膜DNA含量、RNA含量、乳糖酶活性和麦芽糖酶活性都较饲喂牛乳组仔猪显著提高（P<0.05）。

Compared with I group , the content of DNA and RNA in small intestine mucosa and the activity of lactase and maltase were increased significantly ( P < 0.05 ) .

来源于Aspergilluscandidus的乳糖酶基因的克隆及序列分析

Cloning and Sequencing of Lactase Gene from Aspergillus candidus

与米曲霉ATCC20423的乳糖酶相比，该乳糖酶具热稳定性强、比活性高、pH范围宽等特点。

Compare to the lactase from Aspergillus oryzae ATCC 20423 , the expressed lactase from A. candidus have better enzymatic properties including the high thermostability , high specific activity and wide pH range for enzyme reaction .

各组乳糖酶mRNA水平无统计学差异，提示乳糖酶基因的调控应该是发生在翻译和/或后翻译水平，而并非是在转录水平。

No statistically significant difference in lactase mRNA levels between the 4 age-groups implies that lactase gene is regulated at the translational and / or post-translational levels , but not at the transcriptional level .

乳酸克鲁维斯酵母（Kluyveromyceslactis）乳糖酶基因的克隆、表达及酶学性质分析

Cloning and Expression of the Lactase Gene from Kluyveromyces Lactis and Analysis of Lactase Properties

目的通过测定不同年龄段小鼠乳糖酶活性和乳糖酶mRNA水平，探讨乳糖酶变化趋势及其基因调节途径。

Objective In order to clarify the trend of change in lactase and its gene regulation pattern , the authors determined the lactase activities and mRNA levels in BALB / c mice at different ages .

3周时IL，IH组乳糖酶活力高于CC组（P<0.05）；

In the third week , the lactase activity in IL and IH groups were higher than that in normal control group ( P < 0.05 ) .

结果表明：用SDS-PAGE法及梯度电泳法测定该耐热乳糖酶的分子质量分别为51.1kD和65.6kD；

Results : The purity examined by SDS-PAGE showed as a single band . Its molecular mass measured by SDS-PAGE and native pore-gradient polyacrylamide gel electrophoresis was 51.1 kD and 65.6 kD respectively .

黑曲霉D2-26乳糖酶分离纯化研究

Study of isolation and purification of lactase from Aspergillus niger D2-26

结论：在含有X-gal培养基进行划线培养是筛选产乳糖酶菌株的有效方法，同时该菌株具有嗜热性。

Conclusion : Thermus strains producing thermostable lactase can be effectively obtained by culture with medium containing X gal.

研究背景乳精吸收不良（LM）和乳糖不耐症（LI）确定诊断最可靠的方法是活检直接测定乳糖酶活性，然而其侵入性至今仍难作为常规手段应用。

Background The reliable diagnostic method for lactose malabsorption ( LM ) and Lactose intolerance ( LI ) is the direct measurement of lactase activity with biopsy , but the traumatic nature is too large to use in routine way .

乳糖和矿质元素是哺乳仔猪十分重要的营养素，对7日龄的哺乳仔猪乳糖酶研究结果表明：哺乳仔猪乳糖酶的酶活力常数是65.825μg（ONP）/mL。

Lactose and mineral elements are important nutrients for sucking piglets . As determined in the present study , the enzyme activity constant in the lactase of sucking piglets was 65.825 μ g ( ONP ) / mL · min.

目的：对从栖热菌（ThermusSp.A3）中分离得到的耐热乳糖酶进行纯化及部分理化性质的研究。

Objective : To purify and partially physical-chemical properties of thermostable lactase from the strain of thermus sp A3 were studied in this paper .

目的：为进一步提高产乳糖酶酵母Kluyveromycesfragilis（K.fragilis）株的酶产量，对该菌株的培养产酶发酵条件进行优化试验。

Objective : To study the optimal condition on lactase production by yeast Kluyveromyces fragilis ( K.fragilis ) to increase the enzyme productivity .

生长至3日龄时，正常仔猪和IUGR仔猪间麦芽糖酶和碱性磷酸酶活性没有显著差异，而2组间乳糖酶总活性和氨基肽酶总活性的差异显著。

After naturally suckled with their dams for 3 days , the differences of mucosal maltase and alkaline phosphatase activities between normal piglets and piglets with IUGR were all not significant , but the difference of total activity of lactase and AP was significant .

人和哺乳动物体内的乳糖酶／根皮苷（LPH）水解酶也含有一种芳基-β-葡萄糖苷酶，LPH因其涉及成人型乳糖酶缺乏病&一种常见的人体基因紊乱病而受到广泛关注。

Humans and mammals lactase / phlorizin ( LPH ) hydrolase also contains an aryl - β - glucosidase , as LPH relate to adult-type lactase deficiency-a common human genetic disorders which received extensive attention .

乳糖酶在3-6℃下水解牛乳中乳糖，16h水解率达55%，添加0.01%质量分数的柠檬酸钾使水解时间缩短为14h；

At the temperature of 3-6 ℃, lactase hydrolyzed the lactose in milk at the percentage of 55 % for 16 hours , and adding 0.01 % mass fraction of potassium citrate shortened the duration to 14 hours .