乙酰氨基半乳糖

结论：多肽：N-乙酰氨基半乳糖转移酶基因家族cDNA被成功扩增，并建立了该基因家族的cDNA文库。

Conclusion : N-acetylgalactosaminyltransferases gene family cDNA has been amplified successfully and the cDNA library has been made by means of TOPO-TA cloning .

采用TOPO-TA克隆法建立多肽：N-乙酰氨基半乳糖转移酶基因家族cDNA文库

Foundation of Polypeptide : N-acetylgalactosaminyltransferases cDNA Library by TOPO-TA Cloning

目的：通过实验方法建立多肽N-乙酰氨基半乳糖转移酶基因家族cDNA文库，欲以此为基础对人体不同肿瘤细胞中的该基因家族进行表达谱分析。

Objective : To make the cDNA library by TOPO-TA cloning in order to analyze the differences of N-acetylgalactosaminyltransferases gene expression profile in different human tumor cells .

多肽：N-乙酰氨基半乳糖转移酶2酵母双杂交载体构建及激活检测

Construction of Polypetide N-acetylgalactosaminyltransferase 2 Yeast Two-hybrid Vector and Detection in Yeast Two-hybrid System

在同一肿瘤细胞中，N-乙酰氨基半乳糖转移酶基因家族各个成员的表达也有差异，而且与正常细胞中N-乙酰氨基半乳糖转移酶基因家族的表达显著不同。

In the same tumor cells , each N-acetylgalactosaminyltransferases gene which differs in expression levels , displays noticeable differences from those in normal cells .