The cells were cultivated in M199 / L15 basal medium supplemented with 20 % foetal bovine serum and 10 μ g / mL insulin at the temperature of 28 ℃, the concentration of CO_2 being 4 % for long term and were sub-cultured .
在细胞活力和数量两方面达到最佳平衡。在含20%胎牛血清、10μg/mL胰岛素的M199/L15培养基中，于4%CO2,28℃培养箱中长期培养并传代。
l15