YAC

The Establishment of a Three Dimensional PCR Screening System and Quality Evaluation of Human YAC Library

人类YAC库PCR三维筛选体系的建立及质量考核

Long range physical map of YAC DNA

酵母人工染色体DNA的大尺度物理图谱分析

Modification of YAC Both Arms with Three Genes by Homologous Recombination

利用同源重组对酵母人工染色体左右臂进行多基因修饰

The effect of radiation of Nd : YAC laser on sealing ability in retrograde filling

Nd：YAG激光照射对根尖倒充填术窝洞封闭性影响的初步研究

Studies of Human α - lactalbumin YAC as a High Level Expression Vector of HSA Gene

人α-乳白蛋白基因YAC作为HSA基因高效表达载体的研究

Isolation of Human Genomic Single Copy from the YAC Insert End Using Alu-vectorette PCR

用Alu－VectorettePCR方法从酵母人工染色体末端分离人基因组单拷贝片段

Conclusion 60 Co γ - irradiation can trigger the bystander effect of Yac - ⅰ tumor cell .

结论60Coγ射线照射能诱发肿瘤细胞YacⅠ的旁效应。

This is the first report in China on the method of direct screening of coding sequences from human genomic YAC clones .

报导从酵母人工染色体的人基因组片段直接筛选表达顺序的方法体系。

Construction of a YAC Contig Encompassing G200 Locus via Chromosome Walking

通过染色体步查建立水稻G200座位的重叠群

Identification of YAC clone related to bcr gene by Alu-PCR and fluorescence in situ hybridization

Alu-PCR联合荧光原位杂交技术鉴定bcr基因相关YAC克隆

Construction of Human Genome Megabase YAC

人基因组百万碱基级巨型YAC的构建

Yeast Artificial Chromosome ( YAC ) techniques play an important role in the analysis of human genome and the isolation and cloning of disease related genes .

人工酵母染色体（YAC）技术是人类基因组分析及疾病相关基因的分离、克隆中的关键技术。

Conclusion The YAC can be easily misread by UF-50 apparatus , and microscopic examination can overcome this error .

结论在UF-50尿分析仪检测尿液有形成份时，类酵母细胞是引起检测误差的一个重要原因，易导致临床误诊，镜检可以克服这一偏差。

Explored the method of the YAC transfected into goat fibroblast cell by spheroplast fusion and proved the possibility . of this method .

本研究摸索出了一种高效YAC内基因打靶的新方法，并探索了通过细胞融合的方法将YAC外源基因导入哺乳动物体细胞内的可行性，这为利用YAC进行转基因动物的生产提供了借鉴。

Results PVR has the significant inhibiting effect on the DNA synthesis of S 180 , H 22 , and YAC 1 tumor cells , with dose dependent manner .

结果拟黑多刺蚁对S180，H22，YAC1肿瘤细胞DNA合成均有明显抑制作用（P<0.05），其抑制作用随剂量增加而增强.以对S180肿瘤细胞抑制作用最显著。

The transgenic techniques via YAC and BAC vectors may be another approach to obtain transgenic animals as mammary gland bioreactors with high efficiency and high expression without gene targeting .

YAC和BAC载体转基因技术可能成为避开基因打靶获得高效表达的转基因动物乳腺生物反应器的另一途径。

Objective To study the effect of yeast analogical cells ( YAC ) in the assay result by UF-50 urinary sediment analysis apparatus for improving the accuracy of urine assay .

目的研究类酵母细胞对UF-50尿沉渣分析仪测定误差，提高尿液检测的准确度。

Therefore , it has very important significance to construct the genomic library of endangered domestic animal genetics resources by YAC for protection important resources of gene in molecular level and further study in molecular biology .

因此，利用YAC载体系统构建我国濒危畜禽品种的基因组文库，对于在分子水平保存国家重要的基因资源和深入的分子生物学研究等方面，均具有非常重要的意义。

Objective To study the tumor inhibiting effect and mechanism of PVR by observing its effect on DNA synthesis of S 180 , H 22 , YAC 1 tumor cells and its cytotoxicity on them .

目的通过拟黑多刺蚁对体外S180，H22，YAC1肿瘤细胞DNA合成影响及细胞毒性作用，研究该蚁抑瘤效应及机制。

YAC-based transfer of fibroin gene from Antheraea Yamamai to Bombyx mori ── expression of the YAC clone containing a.yamamai fibroin gene in B. mori

YAC介导的天蚕丝素基因向家蚕的转移&天蚕丝素基因-YAC克隆在家蚕的表达

Methods The YAC 1 cells were cultured with amniotic membrane and their proliferation were observed by MTT automated colorimetry on the lst day , the 3rd day and the 7th day respectively .

方法将YAC-1细胞接种于羊膜基质面，用MTT自动比色法分别于接种后1、3、7d检测羊膜对YAC-1细胞增殖的影响。

Results Amniotic membrane could significantly enhance the proliferation of YAC 1 on the lst day , the 3rd day and the 7th day respectively after having been seeded ( P < 0 01 ) .

结果接种后1、3、7d，羊膜均对YAC-1细胞有明显的促增殖作用（P<0.01）。

Moreover , yeast artificial chromosome ( YAC ) cloning and site-specific mutation techniques can be combined with transgenic technique to investigate the developmental gene regulation of large genes ( clusters ) with mutations in different sites .

转基因动物技术与YAC（酵母人工染色体）克隆及定位突变技术的结合可望阐述大片段基因（簇）中不同部位对发育阶段特异性表达的调控规律。

The existence of YAC sequence in the F , progeny of transgenic B. mori suggested that YAC is a medium for the transfer of fibroin gene . There is only one copy of A.

F2代的转基因家蚕蛾的染色体DNA中同时还存在YAC序列，说明YAC对丝素基因具有介导作用。

Method After preprocessing of urine samples contained blood , semen and abundant YAC , Erythrocytes , leukocytes and sperms were counted by UF-50 apparatus and Neubauer 's hemacytometer .

方法将血液、精液和富含类酵母细胞的尿液标本作预处理后，用UF-50尿沉渣分析仪作沉渣分析，同时用牛鲍氏计数板计数，将分析结果加以比较。

Centromere , telomere and origins of replication are important elements to maintain the mitotic stability of chromosome . The Yeast Artificial Chromosome ( YAC ) was firstly built in 1983 triumphantly by using these elements of yeast .

着丝粒、端粒和复制起点是保持染色体在有丝分裂时稳定性的重要组分，利用酵母染色体的这些组分，1983年人们首次成功地构建了酵母人工染色体（YAC）。

The 51 Cr release assay from YAC 1 cells was used to determine the splenic NK cell activity and the double staining of ABC method was employed to observe the immunoreactive expression of Fos , arginine vasopressin and tyrosine hydroxylase .

采用YAC1细胞51Cr释放法测定脾脏NK细胞的活性。用免疫细胞化学观察脑内有关核团的Fos表达，以及PVN和LC中Fos表达阳性的神经元的性质。

Given the high capacity of yeast artificial chromosomes ( YAC ) and bacterial artificial chromosomes ( BAC ), using YAC and BAC as transgenic vectors may be an ideal tool because they can contain all major important elements of lacto protein .

酵母人工染色体（YAC）和细菌人工染色体（BAC）具有容量大的特性，可以将乳蛋白的整个基因座包括所有调控序列全部装载进去，有可能克服位点效应的影响，是一种理想的载体。

It have been reported that the 210 kb yeast artificial chromosome ( YAC ) including the human a - lactalbumin gene could direct the transgene to express in a position-independent manner , therefore is an excellent expression vector to produce transgenic animal bioreactors .

根据报道含完整人α-乳白蛋白基因的YAC载体能指导外源基因的乳腺特异性高效表达，是生产动物乳腺生物反应器的理想表达载体。

Results Severer injury was induced in Yac - ⅰ cells cultured in the media pre-irradiated with different doses of γ - rays than that in Yac - ⅰ cells cultured in unirradiated medium , as shown by increased sensitivity to murine splenic NK cells ( P < 0.01 ) .

结果应用不同剂量60Coγ射线照射的细胞培养液来培养YacⅠ细胞，引发该细胞不同程度的损伤，表现在YacⅠ细胞对小鼠脾NK细胞的敏感性增强（P<0.01）；