QPCR

Selection of Control Genes in Real-time qPCR Analysis of Gene Expression in Sugarcane

甘蔗基因表达定量PCR分析中内参基因的选择

Validation of array results was carried out by real-time quantitative PCR ( QPCR ) .

实时定量RT-PCR方法验证芯片结果。

Meanwhile , both of the two real-time QPCR have the quantitative function compared with the conventional PCR .

两种技术相对于普通PCR技术均具有定量功能。

The mRNA levels for target gene were quantified by real-time quantitative polymerase chain reaction ( qPCR ) .

实时荧光定量PCR检测靶基因mRNA表达水平。

QPCR is more widely used for its multiple advantages of accuracy , sensitivity , reproducibility , and high throughput .

实时荧光定量PCR具有准确、灵敏、重复性好、通量高等优点，而被研究者广泛应用于基因的表达分析。

Therefore , this study will provide some more reliable reference gene combinations for qPCR of gene expression studies in pig tissues and skeletal muscle development .

因此，本研究为猪的基因表达转录分析qPCR提供了几组较为可靠的内参基因组合方案，可用来比较准确地校正目标基因的表达量。

Select the part of the virulence genes to investigate in order to verify the reliability of the microarray data by realtime qPCR .

选取部分毒力相关基因，进一步通过荧光定量PCR验证了芯片结果的可靠性。

By means of qPCR and Western Blot , we validated its mRNA and protein expression level amongst a list of breast cancer cell lines .

通过qPCR与WB，我们在一系列乳腺癌细胞系中验证了TBRG4的mRNA及蛋白表达水平。

In B. mori , joining exogenous genes to normalize the qPCR data is the suitable method in induction experiments to test the expression of genes .

选择加入外源参照基因对qPCR数据进行归一化处理，是家蚕诱导实验中检测基因表达的合适方法。

QPCR could be used to determine the copy numbers of KIR gene , and it was a helpful supplementary tool for certain KIR haplotype analysis .

荧光定量PCR技术可以定量测定个体携带的KIR基因的相对拷贝数，对于家系分析KIR单体型是一有益补充。

QPCR assay was applied to detect the expression levels of miR-26 in the myocardial tissue and plasma in TAAC rats . 3 .

QPCR法检测TAAC大鼠心肌组织和血浆miR-26的表达水平。

This article also proved that Hvl was expressed in HCC by real - time qPCR and immunohistochemical . We look forward to discovering the new target genes of cancer clinical diagnosis .

本文亦通过实时荧光定量PCR技术和免疫组化染色法检测Hvl在肝癌中的表达情况，以期待寻找一个新的肿瘤临床诊断的靶基因。

Methods : The content of serum HBV DNA was assayed in 350 patients with HBeAg negative chronic hepatitis B through quantitative polymerase chain reaction ( QPCR ) based on immunofluorescence .

方法：用免疫荧光定量PCR法检测350例HBeAg阴性慢性乙肝HBVdna含量。

Second , the standard method used QPCR compared three different DNA extraction methods , the overall performance and price options that can be applied to the detection kit for practice , for the next step of the experimental operation .

其次，采用QPCR的标准方法对比三种不同的核酸提取方法，综合性能和价格选择出可应用于检测实践的试剂盒，以进行下一步的实验操作。

This research take the Bombyx mori as the object of study , through the current real-time quantitative PCR study of silkworm commonly used endogenous reference gene analysis , and compare with the dual-spike-in qPCR method .

本研究以家蚕为研究对象，对目前家蚕实时定量PCR研究中常用的内源参照基因进行分析，与双跟踪标定定量PCR方法进行比较。

Objective : To detect and analyze the effects of medical intervention for non-alcoholic fatty liver disease ( NAFLD ) using reverse transcription ( RT ) and real-time quantitative polymerase chain reaction ( real-time qPCR ) .

目的：以逆转录（RT）和实时定量聚合酶链式反应（Real-TimeQPCR）检测和分析药物对于非酒精性脂肪肝病（NAFLD）干预效果。

Finally , loop-mediated isothermal amplification method cf QPCR standard methods , the specificity and sensitivity experiments , was screened for two endogenous genes , 4 ring detection of exogenous gene mediated isothermal amplification primers .

最后以环介导等温扩增法比照QPCR的标准方法，进行特异性和灵敏度实验，筛选得到了对2个内源基因，4个外源基因检测的环介导等温扩增法引物。

This paper focused on two topics in skeletal muscle development : the miR-148a function and its molecular mechanism in skeletal muscle development ; the selection of reference genes for the normalization of qPCR gene expression in porcine skeletal muscle at different development stages .

本文围绕骨骼肌发育这一主题集中探讨了两个问题：miR-148a在骨骼肌发育中的作用及其作用机理；不同发育时期猪骨骼肌基因表达分析技术qPCR中内参基因的选择。

Real-time quantitative PCR ( qPCR ) is a new type of nucleic acid quantitative technique which developed on the basis of traditional PCR technique , it is widely used in gene expression analysis with advantages of high sensitivity , specificity , rapidity , quantification and accurately .

实时荧光定量PCR（qPCR）是在传统的PCR技术基础上发展起来的一种新型的核酸定量技术，具有灵敏度高，特异性好，快速准确等优点而被广泛应用于基因的表达分析。

The total RNA of adipose tissue was extracted and reversely translated into cDNA . The relative level of tumor necrosis factor α ( TNF - α) mRNA was detected in the real-time qPCR . The data difference between two groups was analyzed by t-test .

从大鼠脂肪组织提取总RNA并逆转录成为cDNA，通过real-TimeQPCR检测肿瘤坏死因子α（TNF-α）mRNA的相对水平，以t检验分析两组间数据的差异。

Real-time quantitative PCR ( qPCR ) analysis carried out on mRNAs from leaves at different time points post-inoculation with two fungal pathogens ( Peronospora parasitica and Alternaria brassicicola ) and two signal transduction chemicals , salicylic acid ( SA ) and methyl jasmonate ( MeJA ) .

实时定量PCR法对两种真菌（霜霉病菌和黑斑病菌）以及两种信号传导物质SA和茉莉酸甲酯（MeJA）诱导该基因表达进行分析。