D2C

Networked Marketing Operation Model Based on A2C & D2C

基于A2C&D2C的网络化销售运作模型

Cell proliferation test and MTT assay were used to determine effects of antibody ( D2C ) and murine McAb on K562 cells .

细胞计数和MTT试验测定嵌合抗体及鼠源性抗体对K562细胞的作用；

Conclusion The antibody ( D2C ) keeping in liquid nitrogen for tow years production from the transfectomas is stable and specific .

结论由我们制备的在液氮中冻存2年的分泌抗CD71人鼠嵌合抗体（D2C）的转染瘤细胞体外生长良好，抗体分泌稳定，产量高，特异性强。

Objective To investigate the anti-tumor effects in vitro and in vivo distribution of the human / murine chimeric antibody ( D2C ) .

目的研究人鼠嵌合抗体D2C的体外抗瘤活性和体内分布。

Objective To study the effect of an anti-CD71 mouse / human chimeric antibody ( D2C ) in activation of lymphocytes in vitro , and to compare with murine monoclonal antibody .

目的通过体外实验探讨抗CD71人鼠嵌合抗体对活化淋巴细胞效应的影响，并与其鼠源性单克隆抗体进行比较。

Conclusion Antibody ( D2C ) and murine McAb can induce K562 apoptosis by the pathway of mitochondrial death , the induction of apoptosis would be related with interfering iron transportation in cells .

结论抗CD71人鼠嵌合抗体可通过线粒体死亡途径诱导K562人红白血病细胞凋亡，其诱导凋亡可能与其干扰细胞铁离子转运有关。

Objective : To study the specificity , productivity and tumor-induced apoptosis of an anti-CD71 mouse / human chimeric antibody ( D2C ) secreted by transfectomas kept in liguid nitrogen for tow years .

目的：研究在液氮中冻存2年的转染瘤细胞株（D2C）复苏后其分泌抗CD71人-鼠嵌合抗体的特异性、分泌量及诱导肿瘤细胞凋亡的效应。