非结构蛋白

口蹄疫病毒非结构蛋白定量检测ELISA方法的建立

The Establishment of Quantitative ELISA of Foot-and-Mouth Disease Virus Non-structural Protein 3B

人肿瘤细胞株中细小病毒H1的DNA扩增与非结构蛋白NS1基因的表达

DNA amplification and non-structural protein NS-1 gene expression of parvovirus H-1 in human cancer cell lines

用PCR技术克隆细小病毒H－1部分非结构蛋白基因

Cloning of Parvovirus H-1 NS Partial Gene by PCR

HCV非结构蛋白和结构蛋白在复制和感染中的作用

The Role of Non-structural and Structural Proteins in HCV Replication and Infection

其ORF编码3种结构蛋白（C，prM和E）和7种非结构蛋白（NS）。

The ORF encodes 3 structural proteins and 7 nonstructural proteins .

禽呼肠孤病毒σNS非结构蛋白基因的克隆和表达

Cloning and expression of nonstructural protein gene σ NS of avian reovirus

柯萨奇B4病毒非结构蛋白P2C基因的克隆与表达

Cloning and Expression of Gene Encoding Nonstructural Protein P_2C of Coxsackievirus B4

应用巢式聚合酶链式反应技术检测孕妇人微小病毒B19非结构蛋白DNA

Detection of human parvovirus B19 nonstructural protein DNA by nested-polymerase chain reaction in pregnant women

成熟病毒粒子包含11节段双股RNA基因组，可编码6种结构蛋白和5种非结构蛋白。

Mature virus particles contain an 11-segmented double-stranded RNA genome , which encodes six structural and five nonstructural proteins .

猪脑心肌炎病毒非结构蛋白2C的原核表达与间接ELISA方法的建立

Prokaryotic Expression of Nonstructural Protein 2C of Porcine EMCV and Establishment of Indirect ELISA

轮状病毒为双链RNA病毒，基因组含11个RNA节段，编码6个结构蛋白和5个非结构蛋白。

The genome of rotavirus consists of 11 segments of double-strand RNA , encoding 6 structural and 5 non-structural proteins .

口蹄疫病毒非结构蛋白P3区的基因序列及分析

Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus

SARS冠状病毒非结构蛋白Nsp的原核表达

Prokaryotic Expression of SARS Coronavirus Nonstructural Protein 9

结论非结构蛋白DNA巢式PCR检测方法是一种特异、敏感、简便、快速诊断B19病毒感染的新方法。

Conclusion The new nested-polymerase chain reaction assay is a sensitive , specific and convenient method in detecting human parvovirus B19 infection .

口蹄疫病毒非结构蛋白3B真核表达载体的构建及表达

Construction and expression of FMDV non-structural protein 3B gene eukaryotic vector

基因组由11个片段的双股RNA组成，分别编码11种病毒蛋白（6种结构蛋白和5种非结构蛋白）。

The virus particle contains 11 double standed RNA genome , which encodes 11 viral proteins ( 6 structual proteins and 5 non structual proteins ) .

其中非结构蛋白3A的基因长度为459bp，编码153个氨基酸；

A protein gene was composed of 459 nucleotides , encoding 153 amino acids .

亚洲1型口蹄疫病毒RS株非结构蛋白P3区基因特征分析

Analysis of Gene Characteristics of the Nonstructural Protein P3 Coding Region of Foot-and-mouth disease virus Type Asia 1 RS Strain

因此可以建立一种特异检测甲肝病毒非结构蛋白抗体的ELISA方法来表征病毒复制的发生。

Therefore , an ELISA was developed that is specific for antibodies to the nonstructural protein of HAV and thus serves to document the occurrence of viral replication .

目的研究柯萨奇B组3型病毒（CVB3）中国分离株结构蛋白VP4、非结构蛋白3D基因序列及变异性。

Objective To investigate the sequence and variation of VP4 and 3D gene of a coxsackievirus B3 Chinese isolate ( CVB3-Chi ) .

口蹄疫病毒非结构蛋白3B单克隆抗体的制备与鉴定

Preparation and Identification of Monoclonal Antibody ( MAb ) against Non-structural Protein 3B of Foot-and-mouth Disease

非结构蛋白包括2C多肽、3C半胱氨酸蛋白酶和3DRNA依赖的RNA聚合酶样区等结构。

The nonstructural protein contains amino acid motifs of 2C polypeptide , 3C cysteine protease , and 3D RNA dependent RNA polymerase .

蓝舌病病毒非结构蛋白NS1基因在各型中具有高同源性，可作为群特异性检测的依据。

Non-structure protein ( NS1 ) of bluetongue virus demonstrates high homology in its different serotypes and this can be base for group-specific detection .

口蹄疫病毒O/Laos/00株非结构蛋白3A基因特征分析

Characteristics of the nonstructural protein 3A gene of foot-and-mouth disease virus O / Laos / 00 strain

细小病毒H-1非结构蛋白NS1在人肝癌细胞内的超显微结构定位

Ultrastructural localization of parvovirus H-1 nonstructural protein 1 in human hepatoma cells

本文成功构建CVB3结构与非结构蛋白的重组质粒DNA疫苗，为进一步研究其免疫效果奠定了基础。

DNA vaccines of CVB3 structural and nonstructural proteins were constructed , which provides the basis on the further study their immune responses in vivo .

目的：构建柯萨奇B4病毒非结构蛋白P2C重组质粒。

Objective : To construct the recombinant nonstructural P_2 C plasmid in coxsackie B4 virus .

这个检测方法也利于我们更好地了解非结构蛋白抗体在机体对HAV感染的免疫应答中所起的作用。

This assay may facilitate a better understanding of the role of the nonstructural antibodies in the immune response to HAV infection .

登革病毒非结构蛋白NS3研究进展

Advances in Research on the Non-structural 3 Protein of Dengue Virus

方法：收集40例早期自然流产和70例正常早期妊娠孕妇的血清，应用套式PCR技术检测两组孕妇血清中的B19病毒非结构蛋白DNA。

Methods : The parvovirus B19 nonstructural protein DNA was assayed by PCR in sera of 40 pregnant women with first-trimester spontaneous abortion and in sera of 70 normal pregnant women in the first-trimester .