铰链区

其分子结构由4个功能区组成，分别是A/B区、DNA结合区域、铰链区和配体结合区域，每个区域都有其各自不同的功能。

The structural features of TRs include four functional domains : A / B domain , DNA-binding domain , hinge region and ligand-binding domain . Each domain has its respective function . Referring to the self-guard mechanism of G.

人IgG3上游铰链区/p53四价功能域融合基因的构建及空间构象分析

Construction and space conformation analysis of the fusion gene of human IgG3 upper hinge region and p53 tetramerization domain

其发病机制至今仍不清楚，新近的研究提示IgAl分子铰链区O-联接糖基化过程异常可能是IgA肾病重要的发病机制之一。

Recently aberrant O-glycosylation of serum IgAl is presumed to be one of the main pathogenesis of IgAN .

结论人IgG3上游铰链区/p53四价功能域融合基因的成功构建，为进一步研制多价抗体奠定了基础。

Conclusion Successful construction of the fusion gene mentioned above laid the foundation for further preparation of multivalent gene engineering antibody .

方法：以8个甘氨酸作为铰链区，运用over-LapPCR的方法将NIF与KGF连接，获得双重抗肺纤维化基因嵌合重组体NKM的基因序列。

Methods : To obtain the sequence of NKM , we utilized 8 × glycin for hinge region to connect NIF with KGF by over-lap PCR .

目的构建人IgG3上游铰链区/p53四价功能域融合基因，并进行空间构象的分析。

Aim To construct the fusion gene of human IgG3 upper hinge region and p53 tetramerization domain and to analyze its space conformation .

进一步将两个改形基因与c-myc及人IgG3'CL铰链区基因融合在大肠杆菌BL21（DE3）中表达，复性后的融合表达蛋白仍表现为具有较高与CD28分子的结合活性。

Two genes were fused with c-myc tag and the hinge region of human IgGS'CL gene , and expressed in E. coli BL21 ( DE3 ) . The antigen binding affinity and specificity of renatured fusion protein were determined .

为构建和表达抗人CD3单链抗体（scFv）人p53四聚功能域融合基因，选用人IgG3上游铰链区作为抗人CD3scFv和人p53四聚功能域之间连接的linker。

To construct and express anti human CD3 single chain Fv antibody ( scFv ) / human p53 tetramerization domain fusion gene , human IgG3 upper hinge was selected to be the linker between anti human CD3 scFv and human p53 tetramerization domain gene .

MMP结构由信号肽区、前肽区、催化区、铰链区、血红素结合蛋白样区组成，后者可能介导MMP与细胞外基质成分或TlMP结合，并决定底物特异性。

The the MMP structures by the signal peptide region , the propeptide region , the catalytic region , the hinge region , the heme-binding protein sample area , which may be mediated by the MMP and extracellular matrix components , or TIMP binding and to determine substrate specificity .

尽管本文采取的策略假定：非弹性作用受限于下部结构中合理详细的铰链区，同样可以考虑支座处采用位移减弱地震力的替代概念。

Although the strategy taken herein assumes that inelastic action is confined to properly detailed hinge areas in substructure , alternative concepts that utilize movement at t he bearings to dissipate seismic forces may also be considered .

结果，这一抗体能与病毒紧密结合，限制铰链区的运动并阻止病毒与宿主细胞膜融合。

As a result , the antibody is then able to achieve a tighter hold on the virus , restrict hinge mobility and impede the ability of the virus to fuse to the membrane of the host cell .