聚丙烯酰胺凝胶电泳技术

方法：应用以明胶为底物的SDS聚丙烯酰胺凝胶电泳技术对92例乳腺癌患者进行尿液MMP的检测。

Methods : MMP in urine of 92 breast cancer patients were detected by substrate gel electrophoresis .

应用醋酸纤维薄膜和垂直板聚丙烯酰胺凝胶电泳技术对四川平武黄牛地方牛种的血红蛋白（Hb）和血清运铁蛋白（Tf）座位多态性进行了分析。

Hematoglobin protein and serum transferin loci polymorphisms of yellow cattle in Pingwu county were analyzed with cellulose acetate film electrophoresis and vertical polyacrylamide gel elec-trophoresis .

采用不连续非变性聚丙烯酰胺凝胶电泳技术对42只岷县黑裘皮羊的血红蛋白（Hb）和转铁蛋白（Tf）基因座的多态性进行了研究。

The polymorphism of hemoglobin ( Hb ) and transferring ( Tf ) of 42 individuals of Minxian Black-fur sheep was detected by using polyacrylamide gel electrophoresis .

采用垂直聚丙烯酰胺凝胶电泳技术对85只小尾寒羊在转铁蛋白（Tf）位点上的6个生长性状进行了研究。

The study on 6 growth characters at Tf polymorphism loci of 85 Small-tail Han sheep was carried out with vertical polyacrylamide gel electrophoresis ( PAGE ) .

应用垂直板聚丙烯酰胺凝胶电泳技术（PAGE），对曼氏迭宫绦虫成虫及幼虫裂头蚴的乳酸脱氢酶同工酶、酯酶同工酶及苹果酸脱氢酶同工酶进行了分析测定。

Three isoenzymes of Spirometra mansoni and its larva , sparganum were compared by means of-polyacrylamide gels electrophoresis ( PAGE ) . The detected isoenzymes are lactate dehydroge-nase ( LDH ) , esterase ( EST ) and malate dehydrogenase ( MDH ) .

利用改进的乳酸聚丙烯酰胺凝胶电泳技术对247个人参（PanaxGinsengC.A.Meyer）及西洋参（P.quinquefoliumL.）

Systematic electrophoretic analysis of alcohol-soluble proteins and salt-soluble proteins of 247 Panax ginseng C. A. Meyer ( P.g ) and P. quinquefolium ( P.q ) germplasms seed was carried out by an improved lactate-polyacrylamide gel electrophoresis .

利用SDS聚丙烯酰胺凝胶电泳技术，对采自内蒙古、河北、山西、宁夏和甘肃5个省3种2变种的40个胡枝子属植物野生居群种子蛋白进行遗传多样性研究。

Genetic diversity of seed storage protein was estimated on 40 wild Lespedeza populations ( 3 species and 2 varied species ) from Inner Mongolia , Hebei , Shanxi , Ningxia and Gansu provinces , using SDS-PAGE techniques .

应用水平板等电聚焦（IEF）聚丙烯酰胺凝胶电泳技术，开展了2组8份大白菜不育系和保持系花、4级蕾、叶片的EST、ACP、POD同工酶和全蛋白的比较研究。

Isoelectric focusing ( IEF ) electrophoresis technique was used in 2 sets of 8 Chinese cabbage CMS types and maintainers for EST , ACP , POD and total protein analysis in flowers , different buds , leaves in order to study differences between CMS and maintainer .

以改良十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术（1-D-SDS-PAGE）为基础，分析凝胶性质、试验试剂、试验条件对电泳结果的影响，获得高效低成本、分辨效果佳、重复性好的鉴定方法。

The experiment is based on improved 1-D-SDS-PAGE method . Analysing electrophoresis results which are effected by glue properties , experimental reagents and experimental conditions , and selecting a suitable judging way which is high efficiency but low cost , high segregation and good repetition .

SDS－聚丙烯酰胺凝胶电泳技术的改良及其应用

An improved method for SDS-PAGE and its application

用马铃薯块茎全蛋白聚丙烯酰胺凝胶电泳技术鉴定品种的真实性和纯度

Testing trueness and purity of potato variety by tuber total protein polyacrylamide gel electrophoresis technique

种子蛋白乳酸尿素聚丙烯酰胺凝胶电泳技术及其对向日葵自交系和杂交种的鉴定

Lactate urea polyacrylamide gel electrophoretic technology for seed proteins and identification of sunflower inbreds and hybrids

本文以聚丙烯酰胺凝胶电泳技术，对1986年浙江省不同地理位置流行的轮状病毒基因组进行比较。

Rotavirus genomes from various areas in Zhejiang province in 1986 were studied by means of polyacrylamide electrophoresis .

目的比较双向电泳和SDS-聚丙烯酰胺凝胶电泳技术分离人血清中蛋白质的效果。

Objective To compare the effect of separating protein in human serum with two-dimentional electrophoresis ( 2-DE ) and SDS-PAGE .

方法利用垂直平板聚丙烯酰胺凝胶电泳技术，作蛋白质、过氧化物酶同工酶、酯酶同工酶分析。

Methods The protein , peroxidase isozyme and esterase isozyme of Radix Platycodon grandiflorum were studied by means of polyacrylamide gel electrophoresis .

采用常规聚丙烯酰胺凝胶电泳技术，对海南吊罗山和霸王岭两个国家级自然保护区野生荔枝等位酶遗传多样性进行了研究。

Allozyme variation and genetic diversity were evaluated among wild litchis sampling from two nature protection areas of Bawangling and Diaoluoshan in Hainan Island .

利用改进的不连续聚丙烯酰胺凝胶电泳技术，分析了洋葱不同类型品种的种子醇溶蛋白。

Based on the improved techniques of polyacrylamide gel electrophoresis , the seed gliadin of the different types of onion varieties was carried out .

运用组织学、组织化学及聚丙烯酰胺凝胶电泳技术，研究了文蛤粘液细胞的类型、分布及组化特性。

The types , distribution and histochemistry characters of mucous cells of Meretrix meretrix Linnaeus were studied by histological and histochemical methods and SDS-PAGE electrophoresis .

采用垂直板聚丙烯酰胺凝胶电泳技术，对正常的和患抖抖病的中华绒螯蟹几种组织中的可溶性蛋白质及乳酸脱氢酶、酯酶和过氧化物酶等同工酶进行了比较分析。

The soluble proteins , dehydrogenase ( LDH ), esterase ( EST ) and peroxidase ( POD ) isozymes , in the tissues of healthy mitten crabs Eriocheir sinensis and of individuals with shiver disease were studied by polyacrylamide gel electrophoresis .

作者应用聚丙烯酰胺凝胶电泳技术分析比较了光培养绿草履虫和暗培养绿草履虫中酯酶、酸性磷酸酶、苹果酸脱氢酶和乳酸脱氢酶四种同工酶的组分及含量的变化。

PAGE was used to analyze and compare the variation of composition and content of four isoenzymes , esterase , acid phosphatase , malate dehydrogenase ( MDH ) and lactate dehydrogenase ( LDH ), of Paramecium bursaria which cultured in light and dark .

2DE（二维聚丙烯酰胺凝胶电泳）技术是蛋白质组学研究中的主要分离技术，其分别以蛋白质的等电点和分子质量这两个独立的参数为基础，实现蛋白质混合物的分离。

Two-dimensional polyacrylamide gel electrophoresis is the key separation technique in proteomics research , which is designed by protein character : molecular weight and PI .

目的通过聚丙烯酰胺二维凝胶电泳技术，全面展示小鼠成熟卵丘卵母细胞复合体（COC）的蛋白质表达谱。

Objective : To establish two-dimensional polyacrylamide gel electrophoresis for displaying the full-scale protein expression spectrum of mouse mature oocyte-cumulus complex and evaluate the application of this map in the study of ovarian follicle development .

方法采用盆栽控制试验，利用垂直板聚丙烯酰胺凝胶电泳分析技术进行同工酶分析。

Methods : Pot control was adopted in the experiments and vertical slab polyacrylamide gel electrophoresis technique was utilized in the POD analysis .

方法应用PCR和非变性聚丙烯酰胺凝胶电泳分型技术，并检验各基因座女性基因型频率分布是否符合HardyWeinberg平衡，计算法医学常用各种概率。

Methods The PCR products were detected by the polyacrylamide gel electrophoresis and silver staining method . Hardy-Weinberg equilibrium of females was tested and every forensically interested value was calculated .

应用聚丙烯酰胺凝胶垂直板型电泳技术，对内蒙古苜蓿盲蝽属（AdelphocorisReuter）的6种苜蓿盲蝽不同种群的酯酶同工酶（EST）、过氧化物同工酶（POD）、乳酸脱氢酶同工酶（LDH）进行比较研究。

EST , POD and LDH isozymes of six different geographical populations of plant bug ( Adelphocoris ) Reuter were studied using vertical slab PAGE .

采用双向聚丙烯酰胺凝胶电泳和免疫学技术，对大鼠胚胎、新生大鼠和成年大鼠的骨骼肌快肌肌钙蛋白T的同工型进行了研究。

Rat troponin T isoforms were examined in fetal , neonatal and adult rat skeletal muscles using two-dimensional polyacrylamide gel electrophoresis and immunology .

随着双向聚丙烯酰胺凝胶电泳和质谱技术的进步，使蛋白质组分析及其在临床疾病中的应用成为重要的研究方法。

With the improvement of polyacrylamide gel electrophoresis and mass-spectrum technique , proteome analysis has become a important method for clinical disease research .

应用酶活性测定和聚丙烯酰胺浓度梯度凝胶电泳技术，研究了3种不同浓度的重金属胁迫对不同类型水稻萌发种子中α，β-淀粉酶同功酶活性的影响。

In this paper , the α,β amylase activity measuring methods and polyacrylamide concentration gradient gel electrophoresis technique were used to study the effect of heavy metal stress on amylase activity in germinating rice seeds .

微卫星DNA聚丙烯酰胺凝胶电泳和银染技术的探讨

Study on Polyacrylamide Gel Electrophoresis and Silver Staining Methods for Microsatellite DNA

同时采用聚丙烯酰胺凝胶（PAGE）电泳技术，比较了褶纹冠蚌的外套膜、珍珠囊、珍珠粉蛋白与经过培养的组织块蛋白的变化。

The character of proteins from mantle , pearl sac , pearl and cultured tissue were compared using PAGE technique .