羧肽酶

毕赤酵母羧肽酶对重组双功能水蛭素C端的降解作用

The C Terminal of Recombinant RGD-Hirudin Was Hydrolyzed by Yeast Carboxypeptidase

羧肽酶B保存方法及其稳定性比较

Comparing carboxypeptidase B activities and the stabilities with different storage conditions

基因芯片法检测胆囊胆固醇结石病人肝脏羧肽酶E的表达

Expression of hepatic carboxypeptidase E in patients with cholesterol gallstone

研究从猪胰脏中提取的羧肽酶B，在不同使用条件下，采用不同的保存方法，对其酶活力及稳定性进行比较。

We now present a comparative study of the Carboxypeptidase B.activities and the stabilities with different storage conditions .

Ca2+及低浓度的Mg2+对羧肽酶B有激活作用；

Carboxypeptidase B activity was enhanced by Ca ~ ( 2 + ) and low concentrations of Mg ~ ( 2 + );

羧肽酶B是重组胰岛素生产中的一个必需工具酶，同时在蛋白质测序等领域中也得到广泛应用。

Carboxypeptidase B is a necessary enzyme especially in the processing of recombinant insulin and is often widely used in the sequencing of proteins .

分别用羧肽酶B降解和部分胰蛋白酶酶解后的质谱数据进一步证实了以上的修正。

The revised sequence is further confirmed by comparing above FAB map with that of those peptides produced after further step of carboxypeptidase B digestion .

羧肽酶B在毕酵母中的表达、纯化与活性鉴定卫星搭载啤酒酵母糖代谢相关酶类活性变化

Expression , Purification and Characterization of Carboxypeptidase B in Pichia Pastoris ; Activity Changes of Carbohydrate Metabolism Related Enzymes of S. cerevisiae after A Satellite Flight

猪羧肽酶A1酶原的基因克隆及其在毕赤酵母中的表达

Gene Cloning and Expression of Porcine Procarboxypeptidase A1 in Pichia Pastoris

人羧肽酶A1的毕赤酵母可溶表达

Soluble expression of human carboxypeptidase A1 active center in pichia pastoris

羧肽酶A1及其活性中心基因的表达及活性分析

The Expression of Human Carboxypeptidase Al and Its Active Center Gene

已从南极磷虾提取了8种蛋白酶：三种丝氨酸类胰蛋白酶，一种丝氨酸类胰凝乳蛋白酶，以及两种羧肽酶A，两种羧肽酶B。

The extract contains eight proteolytic enzymes : three trypsin-like serine proteinases , one chymotrypsin-like serine proteinase , two carboxypeptidase A enzymes , and two carboxypeptidase B enzymes .

血管紧张素转换酶（ACE）2是近年来新发现的一种单羧肽酶，是已知的第一个ACE同系物。

Angiotensin-converting enzyme ( ACE ) 2 is a novel discovered mono-carboxypeptidase and the first homolog of ACE .

从马铃薯中提取、纯化了羧肽酶抑制剂（CPI），免疫家兔后产生CPI抗血清。

Carboxypeptidase Inhibitor ( CPI ) was isolated and purified from potato , and the antiserum against CPI as obtained by immunizing the rabbits .

羧肽酶B消除C4副带后对湖北地区C4基因频率及家系C4单体型与补体型的检测

Reevaluation of the Frequencies of C4 Alleles , C4 Haplotypes and Complotypes in the Han Families of Hubei District After Removal of C4 Minor Bands by Carboxypeptidase B

目的：应用毕赤酵母表达系统表达人羧肽酶A1（CarboxypeptidaseA1，CPA1）活性中心蛋白。

AIM : To express the human carboxypeptidase A1 ( CPA1 ) active center protein in soluble form in pichia yeast .

人血管紧张素转换酶2（ACE2）是目前已知的惟一的人血管紧张素转换酶（ACE）的同源物，是一种新型的金属羧肽酶，很多特性与ACE截然不同。

Human angiotensin-converting enzyme 2 ( ACE2 ), an only known homologue of human ACE , is a novel metallocarboxypeptidase with characteristics distinct from those of ACE .

目的：利用基因芯片和实时定量聚合酶链反应（real-timePcR）分析胆囊胆固醇结石病人肝脏中羧肽酶E（carboxypeptidaseE，CPE）的mRNA表达，探讨CPE与胆囊胆固醇结石形成可能相关的分子机制。

Objective To survey carboxypeptidase E ( CPE ) mRNA expression by cDNA microarray and real-time PCR in the liver of patients with cholesterol gallstone ( CGS ), and to reveal the role of CPE in the formation of CGS .

利用基质辅助激光解吸飞行时间（MALDITOF）质谱技术，测定羧肽酶Y消化蛋白质和多肽。

The carboxypeptidase digestions of peptides were analysed for C terminal sequences by matrix assisted laser desorption / ionization time of flight ( MALDI TOF ) mass spectrometry .

这一新方法同样可用于在细胞裂解液中分析PKA催化亚基的含量。（4）首次提出了一种基于石墨烯／多肽复合物和羧肽酶降解作用的激酶活性分析方法。

The feasibility of this novel strategy for cellular catalytic subunit of PKA was also demonstrated in cell lysates . ( 4 ) A fluorescent principle for kinase activity assay based on grapheme / peptide complex and peptide digestion was presented .

GS5编码一个假定的丝氨酸羧肽酶。它正向调控籽粒的大小。GS5的高表达具有更大的籽粒。

GS5 encodes a putative serine carboxypeptidase and functions as a positive regulator of grain size , such that higher expression of GS5 is correlated with larger grain size .

计算机辅助的羧肽酶法测定多肽羧端顺序

The Computer-assisted CarboxyPeptidase Method for C-terminal Sequencing of Proteins and Polypeptides

土豆羧肽酶抑制剂编码基因的克隆与表达

Cloning and Expression of Gene Encoding Carboxypeptidase Inhibitor from Potato

一种快速分离纯化土豆中羧肽酶抑制剂的方法

A Rapid Method for Isolation and Purification of CPI from Potato Tubers

产羧肽酶菌株的筛选及其产酶特性的研究

The screening of the carboxypeptidase producing strain and its properties of enzyme producing

羧肽酶菌株的筛选及其酶的分离纯化、酶学性质的研究

Screening of a Strain Producing Carboxypeptidase , Purification and Properties of the Enzyme

当用羧肽酶处理，此肽不被水解。

The peptide failed to undergo hydrolysis when treated with the enzyme carhoxypeptidase .

在大肠杆菌中融合表达重组羧肽酶抑制剂

Fusion Expression of Recombinant Carboxypeptidase Inhibitor in Escherichia coli

猪肾脏羧肽酶性质的进一步研究

Further studies on the carboxypeptidase from swine kidney

抗前列腺特异抗原单链抗体/人羧肽酶A融合基因的构建及表达

Construction and expression of a single chain anti prostate specific antigen antibody fused to human carboxypeptidase A