细胞期

结果原诊断的113例PMF患者各期[包括细胞期、胶原形成期、硬化期和骨骨髓硬化症（OMS）]临床表现差异均无显著性。

Results There was no significant differences on the clinical features among the cellular phase , collagen fiber phase , sclerotic phase and osteomyelosclerosis of 113 previously diagnosed patients .

蛋白激酶B在小鼠1-细胞期受精卵中活性及表达变化

Activity and Expression Changes of Protein Kinase B in 1 - cell Stage Fertilized Eggs of Mouse

蛋白激酶B（AKT）抑制剂对小鼠1-细胞期胚胎分裂的影响

Effect of Protein Kinase B ( AKT ) Inhibitors on Cell Division of Mouse 1-Cell Embryos

PKCζ的特异性抑制剂可以抑制受精卵1细胞期基因的转录和活化（P<005）。

PKC ζ specific inhibitor can also inhibit the early transcription in 1-cell stage eggs ( P < 0.05 ) .

但经OPS方法冷冻的2～8-细胞期的胚胎解冻后能否进一步向后期发育，仍需进行深入的研究。

Additional research is needed to verify that vitrify 2-to 8-cell stage embryos by OPS method can also develop into later stages .

用单胚构建的mRNA差异显示技术，对体外培养的山羊早期2、4、8~16细胞期胚胎的基因表达进行研究，并选择1条在2细胞期胚胎特异表达的条带进行分析。

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell , 4-cell and 8-16-cell stage goat embryos . A specific fragment expressed in 2-cell stage was selected for further analysis .

结果：LN、FN和整合素β3广泛分布于2细胞期至胚泡期胚的细胞表面。

Results : Laminin , fibronectin and integrin β 3 were expressed extensively on the early embryo cell surface from 2 cell stage to the blastocyst stage .

用623μJμm~2能量密度照射二细胞期卵裂沟的卵，多数只能发育至原肠胚，少数发育成为头部分化不全的蝌蚪而死亡。

When the cleavage furrow of the two cell stage was irradiated with an energy density of 623 μ J / μ m ~ 2 , most of the embryos died in the gastrula stage .

结果表明，超排处理DDK雌鼠产生的胚胎，大量处于2细胞期发育停滞。

The results show that DDK ultra-exclusive deal with a large number of female embryos at the 2-cell developmental stagnation and can not conduct a follow-up test .

高粱雄性不育系3197A（3A）在花粉母细胞期进行热激处理后由不育变为可育。

The mitochondria of anther in pollen mother cell stage of cytoplasmic male sterility ( CMS ) line 3197A in sorghum were extracted after heat shock .

方法取试管婴儿助孕技术后异常受精的3原核胚胎，于2~10细胞期进行固定，采用TUNEL法检测3原核胚胎中的凋亡征象。

Methods : The three pronuclear ( 3PN ) human preimplantation embryos were fixed at 2-10 cell stage . TdT-mediated nick end labeling ( TUNEL ) and laser confocal scanning microscopy were used to detect apoptosis signs in embryos .

结果检测20个受精后2~3d的人类卵裂期胚胎，2、4、6、8细胞期胚胎各5个，有7个胚胎表达HLA-G及其部分的异构体mRNA。

Results HLA-G and its isoforms in human cleavage stage embryos : a total of twenty individual cleavage stage human embryos were examined ( five 2-cell , five 4-cell , five 6-cell and five 8-cell ) . Only 7 / 20 embryos expressed HLA-G and its partial isoforms mRNA .

牛体外受精后5-～10-细胞期的胚胎用0.5%链霉蛋白酶处理分离单一卵裂球，然后用100ng/ml长春花碱处理10h，制作染色体标本。

Bovine embryos developing to the 5 ~ 10 cell stage were separated into individual blastomere with 0 5 % protease . After treatment with 100 ng / ml vinblastine sulfate for 10 h , they were prepared for chromosome samples .

试验还表明，母兔交配后28h采胚所获取的胚胎大多处于二细胞期，到交配后32h，胚胎多处于四细胞期。

The results also showed that most of the gained embryos were in 2-cell stage during 28 hours after the rabbits accepted mating , and in 4-cell stage during 32 hours after the rabbits accepted mating .

在培养基中添加pFF可促进猪胚胎体外发育，使发育至8～16－细胞期的胚胎率从3.9%（6／154）提高到10.2%（12／118）。

The addition of pFF to the develpment medium enhanced the development of porcine embryos in culture , increasing the proportion of the embryos passing through the 4-cell block from 3.9 % ( 6 / 154 ) to l0.2 % ( 12 / 118 ) .

280枚人类细胞期胚胎冷冻、复苏及移植结果分析

Analysis of 280 frozen-thawed human embryos and embryos transplant results

哺乳动物早期胚胎体外培养的研究Ⅰ.2-细胞期小鼠胚胎的发育

In vitro Culture of Mammalian Embryos I.Development of Two - cell Embryos of Mouse

小鼠2-细胞期胚胎融合及其发育能力的研究

Fusion and development of two-cell mouse embryos

将部分32－细胞期卵裂球的重组胚移入受体后，产出了3只核移植？

After some nuclear transplant embryos made 32-cell stage nuclei were transferred to the recipients .

胚胎移植结果表明，1-细胞期克隆胚胎有利于受体母猪妊娠，同时可提高克隆效率。

The results showed that 1-cell was improved the pregnancy rate and the cloning efficiency .

次级精母细胞期，线粒体数目开始减少，但体积增大；

At spermatocyte ⅱ stage , the number of mitochondria decreased , but the volume increased ;

正常交配方式中杂合胚胎在8细胞期后发生凋亡现象，并确定为死亡细胞。

Mating the normal mode hybrid embryos died after 8-cell apoptosis , identified as dead cells .

精核蛋白对小鼠1-细胞期受精卵转录的影响

The Effect of Protamine on the Transcription of 1 - Cell Stage Fertilized Eggs in the Mouse

结果：在小鼠受精卵和2-细胞期胚胎中，全部表达14-3-3蛋白，并且为同一亚型；

Results : The expression of 14-3-3 proteins was identified in mouse fertilized eggs and 2-cell stage embryos .

目的了解2细胞期小鼠胚胎与人输卵管上皮细胞体外无血清共培养中的发育状况。

Objective To examine the effect of human oviductal epithelial cells on early mouse embryonic cleavage and growth in vitro .

显微注射实验证实：在青鳉受精卵的单细胞期进行显微注射，标记基因的表达率和胚胎的存活率均较高。

Microinjection experimentation proved that both expression rate of marker gene and the survival rate of transgenic embryos are high .

利用单一卵裂球的染色体诊断牛体外受精5-～10-细胞期胚胎的正常性。

Chromosomal normality and sex were diagnosed in each blastomere of bovine embryos derived from in vitro fertilization ( IVF ) .

小鼠胚胎中软脂酸和油酸等脂肪酸含量与相应细胞期输卵管液和子宫液较为一致。

The contents of palmitic acid and oleic acid in mouse embryos were relatively similar to that in reproductive tract fluids .

通过裂球移植，将16细胞期非洲爪蟾胚胎动物半球的背方裂球和腹方裂球进行异位移值，分别产生具有双背预定命运和双腹预定命运的胚胎。

The pair of dorsal-most animal blastomeres of the 16-cell Xenopus embryo were transplanted to the ventral side of a synchronous recipient .

精原细胞期，精巢小，透明乳白色，生精小管内的生殖细胞以精原细胞为主；

The testis at spermatogonium phase is small and transparent , and the spermatogenetic cells in seminiferous tubule primarily consist of spermatogonia ;