纤维荧光原位杂交

我们探索了DNA纤维荧光原位杂交方法（即DNA纤维FISH）检测该断裂位点的可行性。

We tested the feasibility of DNA fiber fluorescence in situ hybridization ( fiber FISH ) for detection of the breakpoint .

方法用DNA纤维荧光原位杂交（DNAfiber-FISH）技术检测M2型急性髓细胞性白血病（AML-M2）相关易位基因，观察50个间期核荧光信号，并与间期核FISH比较。

Method : DNA Fiber-FISH and interphase FISH were both used to detect translocation genes associated with AML-M_2 . 50 interphase nuclei were observed .

用伸展DNA纤维的荧光原位杂交（Fiber-FISH）技术测定了5Srdna在大麦的基因组中的拷贝数，计算出5Srdna的拷贝数约为408～416。

The copy number of 5S rDNA was determined by extended DNA fiber based on DNA fiber fluorescence in situ hybridization ( Fiber-FISH ) in barley genome . It could be estimated that the copy number was about 408-416 for 5S rDNA .