糖原染色

结果1.体外成功分离和培养出Wilson病病人肝细胞，并通过糖原染色法证实。

Hepatocyte from Wilson patients were isolated and cultured in vitro successfully , and was proved by PAS . 2 .

心肌细胞的PAS糖原染色呈阳性，胞浆内大量的糖原颗粒被染成品红色；

PAS glucogen staining of cardiomyocytes was positive , glucogen in cytoplasm were stained to purple ;

糖原染色亦呈阳性反应。

Positive reaction was also shown in glycogen staining .

细胞化学染色：糖原染色呈强阳性；

Staining for glycogen is strongly positive ;

高碘酸-Schiff糖原染色可见胞浆内存在红色颗粒，呈阳性反应，培养液内尿素氮含量亦明显升高。

Periodic acid Schiff stain could observed that positive red granules existed in the cytoplasm , and the content of urea nitrogen in medium was also obviously increased .

糖原PAS染色和吲哚氰绿（ICG）摄取试验检测肝细胞功能。

Periodic acid Schiff ( PAS ) reaction and indocyanine green ( ICG ) uptake assay were performed to assess the characteristic hepatocyte function of the differentiated cells .

观察到ASBR中单个细菌储存糖原可被染色，连续流反应器中丝状菌则未见此现象。

Dissociated microbe of ASBR which had stored glycogen can be stained , and hyphomycetes in continuous flow reactors cannot be stained .

目的：将国际标准的、稳定可靠的七种酶及糖原细胞化学染色试剂研制为盒式成套试剂。

Objective : Eight enzyme cytochemical stain in international standard , stable , reliable will be researched and produced to kit .