电泳分离
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电解质溶液组成对低分子量阴离子毛细管电泳分离的影响
Effects of Carrier Electrolyte Composition on Capillary Electrophoretic Separation of Low Molecular Mass Anions
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失重飞机上进行的电泳分离实验
The electrophoretic separation experiments on the weightless aircraft
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琼脂糖凝胶电泳分离纯化和制备质粒DNA
The Separation Purification and Preparation of Plasmids DNA by Agarose Gel Electrophoresis
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同步辐射X荧光法测定电泳分离后蛋白条带内的锌
Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation X-ray fluorescence
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综述了用于毛细管电泳分离DNA及测序的聚合物介质的研究进展。
This paper reviews the advances in the research of polymers for DNA separation by capillary electrophoresis .
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方法:选用不同pH的正极缓冲液和样品蛋白上样量,应用非变性聚丙烯酰胺凝胶电泳分离大鼠海马组织蛋白质。
Methods : Nondeture PAGE was used to separate proteins in different pH and loading quantity of protein sample .
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琼脂糖凝胶电泳分离PCR产物,胶回收试剂盒回收分离目的基因片段。
PCR product was separated by agarose gel electrophoresis , then gel extraction kit isolated purpose gene fragments .
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高效毛细管区带电泳分离亲水性(Gly)n小肽
Separation of Hydrophilic Small Peptides ( Gly ) _n by High-Performance Capillary Zone Electrophoresis
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方法采用固相pH梯度双向凝胶电泳分离UVA辐射前后人皮肤成纤维细胞的总蛋白质,凝胶经银染显色后,用ImagingMaster2D图像分析软件进行比较分析,识别差异表达的蛋白质。
After silver staining , the differential expression proteins were analyzed by using Imaging Master 2D analysis ware .
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毛细管电泳分离I~-和IO3~-中的电渗流的控制与调节
Manipulation of Electroosmotic Flow in Capillary Electrophoresis for Iodide and Iodate
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通过琼脂糖凝胶电泳分离的DNAⅠ和DNAⅡ都是双链线状分子,但DNAⅠ分子的长度略长。
Both DNA ⅰ and ⅱ, which were separated by agarose gel electropherogram , were double-stranded linear molecules , and the length of DNA ⅰ was slightly longer .
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考察了背景电解质中硼砂缓冲液的pH和浓度、βCD浓度、有机添加剂甲醇含量及分离电压对手性分离的影响,建立了毛细管电泳分离西孟坦对映异构体的方法。
The influences of the concentration and pH of borate buffer solution , β - CD concentration , methanol content and separation voltage were investigated .
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PCR扩增产物经6%变性聚丙烯酰胺凝胶垂直电泳分离后,银染能检测到多而清晰的条带。
The PCR amplification products were separated on 6 % vertical denaturing polyacrylamide gels . Numerous and distinct bands could be detected by silver staining .
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方法从人血凝块中提取基因组DNA,用聚合酶链反应扩增出APOE基因片段,用限制性内切酶CfoⅠ消化,经聚丙烯酰胺凝胶电泳分离,使之分型。
METHODS The genomic DNA was extracted from the blood clot and the related Apo E gene sequence was amplified by PCR .
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方法:用双向凝胶电泳分离成年雄性ICR小鼠睾丸总蛋白;
Methods : Protein from adult ICR mouse testes was extracted by two dimensional electrophoresis .
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为鉴定莲种子中的高温响应蛋白,从未处理的对照种子和90°C处理24h的种子的胚中提取蛋白经双向电泳分离用于比较蛋白质组学研究。
To identify proteins responsive to high temperature in sacred lotus seeds , both treated and untreated protein samples were resolved by 2D gel electrophoresis for comparative proteomic analysis .
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芯片毛细管电泳分离多种FITC衍生的氨基酸
Separation of FITC-derived Amino Acids by Chip-based Capillary Electrophoresis
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介绍了近年来铀提取方面的技术进展,包括提铀新工艺:超临界CO2流体提取铀,杯芳烃提铀,毛细管电泳分离铀,振荡法萃取铀;
Technical progress of uranium extraction in recent years is introduced , including new processes such as uranium extraction by supercritical CO 2 fluid , calixarene , capillary electrophoresis , oscillation ;
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奶牛血清AKP聚丙烯酰胺凝胶圆盘电泳分离法
Separation of alkaline phosphatase ( AKP ) from dairy cattle sera with polyacrylamide gel disc electrophoresis ( PAGDE ) technique
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反向高效液相色谱(RP-HPLC)与毛细管电泳分离结合ICP-MS和ESI-MS平行检测,已用于表征金属硫蛋白(MTs)中的金属络合物。
Reversed phase HPLC and CE with parallel ICP-MS and ESI-MS have been applied for the characterization of metal complexes with metallothioneins .
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SDS-PAGE凝胶电泳分离、洗脱、透析纯化后,得到纯化的融合蛋白。
After being separated by SDS-PAGE electrophoresis , being eluted and purified through dialysis , the purification of fusion protein pET-21a ( + ) - decorin was obtained .
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叶绿素蛋白复合物凝胶电泳分离,SDS-PAGE,westernblots免疫检测、以及叶绿素荧光诱导动力学结果均确证暗萌发莲苗有光合系统的发育,特别是PSI的出现;
The results from chlorophyll-protein complex separation , SDS-PAGE , Western Blots , and fluorescence induction kinetics all demonstrated the photosystem development , especially the PSI appearance in dark-grown lotus seedlings .
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方法:应用超速离心法分离胆石症患者胆汁泡,运用SDS-PAGE电泳分离其中胆汁泡蛋白,并经HPLC系统纯化,研究其成核活性和N-端氨基酸序列。
Methods : Biliary vesicules were isolated by ultracentrifugation , and their vesicular protein ( s ) isolated by SDS-PAGE and purified by HPLC . The nucleating activity was determined by the nucleation-time method .
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方法用Sebia琼脂糖凝胶电泳分离30名正常人、23例PBC患者和25例其他肝胆疾病患者的血清ALP同工酶,经光密度扫描仪扫描后,分析各型同工酶的相对含量。
Methods ALP isoenzymes were measured by agarose gel electrophoresis in the sera from 30 control subjects , 23 patients with PBC and 25 patients with other hepatobiliary disease .
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再经SephadexG-100、SephadexG-25和聚丙烯酰胺凝胶电泳分离等,从林蛙皮肤中分离获得5种小分子多肽。
Five kinds of active peptides were obtained from Chinese forest frog skin which isolated through sephadex G-100 、 sephadex G-25 and PAGE .
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建立了毛细管电泳分离激光诱导荧光检测(CELIFD)分析分枝杆菌脱氧核糖核酸(DNA)限制性内切酶谱的新方法。
A new method for rapidly detecting restriction enzyme pattern of mycobacterium deoxyribonucleic acid ( DNA ) by capillary electrophoresis with laser induced fluorescence detection ( CE-LIFD ) was developed .
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用双向电泳分离两种细胞蛋白质混合物并进行比较,找出差异点,这些点经过胶内酶切后进行MALDITOF质谱鉴定。
After in_gel protein digestion , the different_expressed spots were detected by matrix_assisted laser desorption ionization_time of flight mass spectrometry ( MALDI TOF MS ) .
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2DEMS途径的自动化,多维色谱整合串联质谱的使用,弥补了一些用双向凝胶电泳分离蛋白质的技术缺陷;
The automation of 2DE MS approach and the use of multidimensional liquid chromatography ( LC ) and tandem mass spectrometry ( MS / MS ) have overcome many disadvantages of two dimensional gel electrophoresis .
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研究了背景电解质、缓冲液pH值、操作温度和分离电压等对电泳分离中运行电流、电渗迁移率、峰形等参数的影响,确定了金属离子电泳分离的最佳实验条件;
The effects of background electrolyte , buffer solution pH , operation temperature and separation voltage on the separation of metal ions in capillary zone electrophoresis were investigated through three parameters : current , electroosmotic mobility and peak shape . Optimum conditions for metal ions separation were selected .
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研究了络合剂α-HIBA的添加及其浓度、背景吸收质浓度、缓冲液pH、进样时间及电压对电泳分离中金属离子的迁移时间、分离度、峰形等参数的影响,确定了最佳实验条件。
The effects of complexing agent α - HIBA and its concentration , background absorbent concentration , buffer pH , injection time and applied voltage on migration time , separation effect and peak shapes of metal ions were studied . The optimum conditions for the determination were selected .