生精小管

大鼠生精小管体视学及PCNA、凋亡表达的发育变化研究

The study of the seminiferous tubule stereology and the expression of PCNA and apoptosis in the rat testes during the postnatal development

研究证明：Fyn蛋白存在于支持细胞的细胞骨架，Fyn缺损可导致生精小管形成延缓，并出现细胞变性。

The lack of Fyn in testis can lead to delayed formatio n of seminiferous tubule and degeneration of cells .

大鼠睾丸生精小管上皮精子发生周期的PAS法判定

Evaluation of Seminiferous Epithelium Stage in Rat Testis by PAS Staining

精原干细胞（SpermatogonialStemcells，SSCs）是指位于睾丸生精小管基膜上既能自我更新维持自身群体数量恒定，又能定向分化形成精母细胞，最终形成精子的一类原始干细胞。

Spermatogonial stem cells were located at seminiferous tubule which not only can maintain the number of themselves but also can differentiate to spermatocyte .

结果出生后1～10d的小鼠睾丸生精小管上皮细胞未见β1整合素的表达；

Results The integrin β 1 was not expressed in testicular seminiferous epithelium of 1 ~ 10 days old mouse .

在胎儿睾丸生精小管中，可见SSEA-1在生殖细胞表面存在显著阳性表达，具有明显特异性。

SSEA-1 specifically expressed on the surface of the gonocytes in the fetal testes .

结果：在2周龄小鼠睾丸生精小管上皮中即可观察到p38MAPK免疫阳性反应，免疫反应阳性细胞为精原细胞；

RESULTS : The immunostaining of p38 MAPK was seen in spermatogonium of 2 week old mice testes .

出生后11d到成年小鼠的不同年龄阶段均可检测到β1整合素在睾丸生精小管上皮细胞中的表达。

From 11 days old to adult mouse , the integrin β 1 was always expressed in testicular seminiferous epithelium .

DBP作用于雄性大鼠后，睾丸支持细胞相关蛋白表达异常，改变了睾丸支持细胞的结构和功能，导致生精小管结构及功能的变化，以致生精功能障碍。

When male rats exposed to DBP , the Sertoli cell-related protein expressed abnormally and the Sertoli cell structure and function altered . This leads to seminiferous tubule changes in the structure and function , also results in spermatogenic dysfunction .

结果6日龄小鼠的生精小管主要包含细胞为精原干细胞、支持细胞，精原干细胞纯化后纯度达80%以上，精原干细胞主要分布在28%～36%的Percoll梯度中。

Result : seminiferous tubules of 6-day-old mouse were mainly composed of spermatogonial stem cells and Sertoli cells . The purity of the SSCs was up to 80 % . SSCs mainly distributed in 28 % ~ 36 % Percoll gradient . 2 .

3,4和5周龄小鼠睾丸仅有个别生精小管上皮可见p38MAPK免疫阳性反应；

Only a few seminiferous tubules were p38 MAPK positive in 3 to 5 week old mice , whereas the expression of p38 MAPK was abundant in the testes of 6 and 7 week old mice .

通过组织学和细胞凋亡的检测我们发现GTW处理后的雄鼠生精小管中生殖细胞总数随着药物剂量的增加而显著减少，而细胞凋亡率显著增加。

By the detection of histology and apoptosis we found a significant dosage-dependent decrease in the total number of germ cells in seminiferous tubules after the GTW treatment , and apoptotic cells were correspondingly increased from low-dosage to high-dosage group .

PrRP在睾丸生精小管和睾丸间质中大量存在，提示PrRP可能直接作用于睾丸，影响雄激素的分泌释放，促进精子的生成成熟。

There is a good deal of PrRP in the seminiferous tubules and leydig , indicating that PrRP may act on testis directly , influence the secretion and releasing of androgens and promote formation and maturation of sperm .

精索静脉曲张大鼠生精小管生精上皮的超微结构研究

Study on ultrastructure of seminiferous epithelium in rats with varicocele

成年小鼠生精小管及睾丸的冷冻保存

Cryopreservation of Adult Mouse Seminiferous Tubules and Whole Testes

目的：探讨睾丸生精小管内精原细胞瘤的诊断和治疗。

Objective : To explore the diagnosis and treatment of testicular intratubular seminoma .

c：九周点，组1生精小管结构正常。

The nine weeks time , seminiferous tubule are normal in the first group .

扫描电镜观察生精小管内精子密度。

The density of spermatozoons in seminiferous tubule was observed with scaning electron microscopy .

在小鼠的生精小管内，精原细胞和各级精母细胞均有催产素阳性产物表达。

In the mouse seminiferous tubules , all of spermatogonium and spermatocyte expressed OT positive products .

b：六周点，组1小鼠睾丸与生精小管情况是正常的。

The six weeks time , testis and seminiferous tubule are normal in the first group .

各处理组生精小管上皮均变薄，管腔增大。

In each group of treatment , the spermatogenic epithelium was thinner , the lumen became larger .

HE染色：12周胎儿睾丸组织尚未分化，呈索状，14周胎儿睾丸组织隐约可见到睾丸小叶，但生精小管不能区分，16周始见生精小管结构，但无管腔。

HE staining : The tissue of testes of 12 weeks have not differentiation , as cord .

橄榄油使用组生精小管破坏，生精细胞层减少，看不到精子。

The olive oil group , seminiferous tubule damaged , spermatogenic cell layer reduced and no sperm appeared .

睾丸生精小管干细胞移植受体模型建立与移植技术改进

Establishment of Recipient Mouse Model of Stem Cell Transplantation into Testicular Seminiferous Tubules and Improvement of Transplantation Techniques

大鼠生精小管组织结构增龄变化的研究

Study on the Tissue Structure Changes of the Seminiferous Tubules in the Testis of the Rat with Aging

组6小鼠生精小管排列较整齐，少见生精细胞脱落，有精子存在。

Seminiferous tubule distribute uniformly , spermatogenic cell shed rarely , and some sperm exist in the sixth group .

Ⅳ期，成熟精子贮存越冬期，11月至翌年2月，成熟精子贮存在生精小管中；

In stage ⅳ from November to next February , mature spermatozoa were observed to store in seminiferous tubules .

饮酒对小鼠睾丸生精小管一氧化氮合酶、增殖细胞核抗原表达及细胞凋亡影响的研究

The effect of alcohol on the expression of enos , pcna and cell apoptosis of seminiferous tubules in the mouse testes

精原细胞期，精巢小，透明乳白色，生精小管内的生殖细胞以精原细胞为主；

The testis at spermatogonium phase is small and transparent , and the spermatogenetic cells in seminiferous tubule primarily consist of spermatogonia ;

胚胎发育阶段和出生后10天以前小鼠睾丸生精小管上皮细胞不表达β1整合素。

Integrin β 1 was not expressed in seminiferous epithelium of fetal mouse testes and testes of 1 ~ 10 dpp mouse .