外植体

SOD和GOD处理均可以使外植体褐化提前。

SOD and GOD deal with can make the explant browning in advance .

目的：以怀牛膝的子叶为外植体，以愈伤指数与齐墩果酸含量优选最佳MS培养基。

Objective : To select the best MS medium .

叶绿素a和b含量却逐渐降低，与外植体的颜色变化相符。

The content of chlorophyll a and b decreased as explant turned black , in accordance with the colour change of explant .

结果表明，以离体去皮根段为外植体，MS为基本培养基，诱导愈伤组织较为适宜。

Callus was efficiently induced from in vitro root segment devoid of rhizodermis on MS medium .

以生长于两种不同MS培养基上的诸葛菜无菌苗下胚轴为外植体，研究不同外植体，不同激素组合对下胚轴高频率再生的影响效应。

Violaceus were cultured to study the effects of explant and hormone on the frequency of shoot regeneration .

以金叶日本冬青新梢茎段为外植体，探讨不同部位新梢、不同激素组合、糖源以及培养基pH值对其愈伤组织诱导及分化的影响。

In this experiment the effect of hormone combination , sugar source and pH on callus induction and differentiation was investigated .

叶片外植体最佳预培养时间为2d，此时抗性愈伤率最高，为71.25%。

For leaf optimum time of pre-culture was 2d . The highest frequency was 71.25 % .

升汞连续两次消毒（6+6）min是曼地亚红豆杉外植体消毒的适宜时间。

Continuously sterilize twice ( 6 + 6 ) min with mercuric chloride is the suitable sterilizing time .

1以东方百合品鳞片为外植体，采用MS培养基和不同外源激素配比，进行组织培养，研究其茎尖分生组织分化。

Meristem culture of Oriental Hybrid Lily through its scales was studied in MS medium with different external hormone treatments .

KT对白花草木樨不同外植体愈伤组织培养的影响

Effects of KT on culture tissue of different explants of Melilotus suaveolens

以菊花珍品绿牡丹的茎段为外植体，MS为基本培养基，进行了离体培养。

Tissue culture of chrysanthemum Curiosa-Green peony was carried through using MS as the basic medium and stem sects as explants .

低温预处理（2℃，1～30天）促进休眠顶芽破裂和自由生长芽形成。C型外植体为最佳接种类型。

The low-temperature pretreatment ( at 2 C for 1 to 30 days ) could accelerate the bursting of resting buds and the formation of free growth shoots .

附加BA和NAA，外植体形成丰满的愈伤组织。

With BA and NAA induces full-grown calli .

以茎尖为外植体，采用MS培养基为基本培养基，适当调整激素浓度，进行白网纹草丛生芽诱导技术及外植体消毒技术研究。

Stem-point is used as explant . And medium MS is used as basic medium . Concentration of internal secretion is adjusted properly .

M99上的外植体，胚珠内尤其是珠心组织中DNA、RNA含量增加，薄壁细胞内DNA含量逐渐减少；

In explants of M99 , DNA and RNA content increased in ovules , especially in nucellus .

蝴蝶兰叶片外植体褐变过程中PAL基因的表达变化

Phenylalanine Ammonialyase ( PAL ) Gene Expression Correlated with Phalaenopsis sp. Leaf Explant Browning

植物组织培养基的pH值一旦外植体植入就开始发生改变，直到达到某一平衡点。

It has been shown that the pH of agar medium changed steadily from the time when the explants were introduced until an equilibrium was reached .

采用毛白杨三倍体幼叶作为外植体进行组织培养，以MS为基本培养基获得了再生植株。

Regenerating plants were produced from immature leaves of the triploid of Populus tomentosa by tissue culture on MS basic medium with different plant hormone .

四倍体龙牙百合鳞片叶直接形成不定芽和体细胞胚的培养及不定芽直接发生的生理生化特性测定结果表明：在MS培养基中添加一定浓度的6－BA可以诱导外植体直接分化不定芽。

The direct formation of indefinite buds and somatic embryoes from scale leaf and the qualities of physiology and biochemistry of the period of bud formation were investigated .

认为：外植体消毒方式、外植体切割方式、PVP均对此有影响。

Disinfecting way of explants , cutting way of explants , PVP all have effect on explants ' browning .

方法以高羊茅（Fes-tuca.arundinacea）成熟种子为外植体，以MS为基本培养基，进行高羊茅胚性愈伤组织诱导、继代、分化及生根培养。

Methods Use mature seeds of fescue arundinacea and MS medium were respectively as explants and basical medium to perfume embryonic callus induction , subculture , differentiation and rooting medium .

以叶片为外植体诱导愈伤组织，2，4&D的效果好于NAA和IBA；

Adding 2,4-D in medium can more effectively induce callus than NAA and IBA from leaves in vitro .

利用根癌农杆菌介导，以下胚轴为外植体把ACC解氨酶基因转入青花菜栽培品种上海2号，获得了转基因再生植株。

ACC deaminase gene was successfully introduced into broccoli ( Brassica oleracea var. Italica ) by Agrobacterium tumefaciens mediated transformation .

对银杏不同外植体愈伤组织生长进行研究，并应用高效液相色谱（HPLC）法测定黄酮苷含量。

The induction of calli on different explants of Ginkgo biloba and the determination of the flavonol glycosides from its callus by high-performance liquid chromatography ( HPLC ) were studied .

以5a生曼地亚红豆杉当年生枝条的带芽茎段为外植体，用MS作基本培养基，进行NAA和6-BA的单因素浓度梯度组织培养试验。

Tests were made on current-growth branches of 5-year-old Taxus media on MS culture medium with different consistency of NAA or 6-BA .

同时，外植体实验表明，YA1的幼穗和成熟胚培养无任何成花反应，而其幼胚外植体具有特异的花芽再生能力。

However , both immature inflorescence and mature embryo , as cultured explants of YA-1 , did not regenerate any flower organs .

山新杨冬季枝条经FC处理，选择萌发新枝的茎段和叶片为接种外植体；

Stem segments and leaves of new shoot ing , which are from winter shoot treated by FC , was used as inoculating explant .

TDZ对仙客来不同外植体再生的影响

Effect of TDZ on bud regeneration from different explants in Cyclamen persicum

以甘蓝型冬油菜下胚轴和子叶为外植体，研究4℃低温处理幼苗、分化培养基添加MES以及预培养等对遗传转化中外植体褐化的影响。

Treatment of the seedlings in the 4 ℃ refrigerator , MES and preconditioning conditions were investigated for explants browning of winter type Brassica napus .

以MS培养基为基本培养基，附加不同种类和浓度的生长调节物质，对不同器官，不同取材时期的重楼外植体进行愈伤组织的诱导培养研究。

In the study , the explants of pairs of different organs and different growth duration were used to induce callus tissues on MS medium with different sorts and concentrations of growth regulating substances .