土壤杆菌

诱导根癌土壤杆菌vir区基因表达信号分子的构效研究

Structure-activity Research of Signal Factors Inducing Agrobacterium vir Genes

主要研究结果如下：（1）采用RT-qPCR和2-DE技术研究了土壤杆菌31749氮源代谢调控系统和菌体总蛋白对氮源限制环境的应答变化。

The main results are described as follows : ( 1 ) The response of Agrobacterium sp. ATCC 31749 to nitrogen limitation from gene transcription and protein expression was analysed by using RT-qPCR and 2-DE methods .

土壤杆菌中辅酶Q（10）的提取工艺

A Study on Technique for Extracting Coenzyme Q_ ( 10 ) from Agrobacterium

根癌农杆菌，根瘤土壤杆菌农杆菌Ri诱导丹参毛状根培养体系的优化

Agrobacterium tumefaciems Establishment of Hairy Roots Culture System of Salvia

根癌土壤杆菌浓度：5×108CFU／ml；

Density of Agrobacterium tumefaciens , 5 × 108 CFU / ml ;

从月季品种金玛丽、曼海姆、杏花村、梅郎口红的根部肿瘤组织中纯化了9株分离物，根据其在MW选择性培养基上的单菌落形态初步判断其为根癌土壤杆菌。

Nine isolates were purified from the crown galls on rose plants and were characterized as Agrobacterium tumefaciens strains according to the colony morphology on the selective MW medium .

将杨树根癌土壤杆菌菌株（CFCC1001）异戊烯基转移酶基因（ipt）片段克隆和序列测定结果显示与A。

The 427 bp isopentenyl adenosine transferase gene ( ipt ) fragment from poplar crown gall A.

异源nifA~C对根癌土壤杆菌nif基因表达的调节作用

Role of the Heterologous nifA C Product in the Regulation of nif Expression in Agrobacterium tumefaciens

方法：采用基因重组技术构建表达C端含组氨酸标签和内质网滞留信号的M-CSF可溶性受体植物表达载体，通过根瘤土壤杆菌转化烟草，获得转基因植株。

Methods : The plant expression plasmid expressing recombinant human M-CSF soluble receptor with C-terminal His-tag and endoplasmic reticulum retention sequence was constructed . Then the target gene was introduced into tobacco genome by agrobacterium tumefaciens-mediated transformation .

葡萄根癌病是由葡萄土壤杆菌（Agrobacteriumvitis）引起的一种在生产上造成了较大经济损失的世界性病害。

Crown gall of grapevine , caused by pathogenic strains of Agrobacterium vitis , has been a problem with economical losses for extensive damage to grape health and quality all over the word .

根据形态特征观察、生理生化特征测定和16Srdna序列分析结果，将As-1、As-2和As-3菌株鉴定为根瘤菌属RhizobiumFrank，Ag-1菌株鉴定为土壤杆菌属AgrobacteriumConn。

These strains were classified into the genus of Rhizobium Frank and Agrobacterium Conn according to their morphology , physiological and biochemical characteristics and analysis of 16S rDNA sequence .

本研究利用根癌土壤杆菌介导的叶盘法将来自小麦的草酸氧化酶基因（oxalateoxidase，OxO）转入优良杨树树种，以获得对多种病原真菌有广谱抗性的转基因杨树。

This research will mainly focus on with the method of Agrobacterium tumefaciens-mediated transformation , the Oxalate oxidase ( OxO ) gene will be tried to be transferred into good poplar species , in order to obtain the enhanced extensively resistant transgenic poplar lines .

用根癌土壤杆菌S-1、702和C-58从向日葵胚轴、烟草茎和茎髓，胡萝卜根及马铃薯块茎诱发并建立了冠瘿株系。

Crown gall strains caused by Agrobacterium tumefaciens S-1702 , C-58 have been established from sunflower hypocotyls , tobacco stems and stem pithes , carrot roots and potato tubers .

在平皿培养时，能够产生农杆菌素MI15，能抑制葡萄根癌土壤杆菌的生长。

The MI15 strain of Agrobacterium radiobacter can produce Agrocin MI15 which inhibits the growth of Agrobactium tumefaciens , a pathogen causing grape crown gall .

放射形土壤杆菌突变株发酵条件及其可溶性胞外多糖结构的研究

Study on Agrobacterium radiobacter Mutant Strain Fermentation Conditions and Exopolysaccharide Structure

根癌病土壤杆菌诱导转化毛状根能力的评价

Appraisement on the ability of inducing transformed hairy root by Agrobacterium rhizogenes

发根土壤杆菌在植物次生代谢物生产中的应用与进展

Application and Progresses of Agrobacterium rhizogenes in Production of Plant Secondary Metabolites

热凝胶是一种由产碱杆菌或土壤杆菌的某些菌株发酵生产的胞外多糖。

Curdlan is an extra-polysaccharide produced by Alcaligenes faecalis .

抑制根癌土壤杆菌生长和转移的水稻信号分子的鉴定

Identification of Rice Signal Factor Inhibiting the Growth and Transfer of Agrobacterium tumefaciens

发根土壤杆菌体外转化甘草子叶及下胚轴

In vitro transformation of cotyledons and hypocotyls of Glycyrrhiza uralensis Fisch by Agrobacterium rhizogenes

抗氧化剂对根瘤土壤杆菌细胞破碎的研究

Study on optimum conditions of cell disruption of the Agrobacterium tumefaciens strain with antioxidant

啤酒花根癌土壤杆菌生物型的鉴定及生物防治的初步探讨

Identification of biotype of Agrobacterium tumefaciens of hop and preliminary studies on biological control

从山东莱阳苹果园的苹果冠瘿瘤中分离到18株根癌土壤杆菌。

Eighteen strains were isolated from crown galls of apple in Laiyang of Shandong Province .

根癌土壤杆菌新生物变种的鉴定

Identification of new biovar of Agrobacterium tumefaciens

樱桃根癌土壤杆菌及其对土壤杆菌素84敏感性的研究

Isolation and identification of Agrobacterium spp . From cherry crown galls and their sensitivities to agrocin 84

根癌土壤杆菌酶的粗提取物及其防止猪痢疾密螺旋体自凝的效果

Extraction of crude enzyme of the Agrobacterium tumefaciens conn and effect of preventive auto-agglutination for the Treponema hyodysenteriae

细菌纤维素是由醋酸杆菌属、根瘤菌属、土壤杆菌属、八叠球菌属等的某些细菌在一定条件下产生的，其中最有代表性的细菌是木醋杆菌。

Bacterial cellulose was synthesized by bacteria belonging to the genera Acetobacter , Rhizobium , Agrobacterium , and Sarcina , etc.

利用发根土壤杆菌（agrobacteriumrhizogenes）在甘蓝、花椰菜、抱子甘蓝和油菜的下胚轴切段上进行遗传转化。

Genetic transformation by Agrobacterium rhizogenes was carried out using hypocotyl segments of cabbage , cauliflower , Brussels sprouts and oilseed rape .

葡萄根癌土壤杆菌诱导的葡萄、毛叶曼陀罗冠瘿瘤的离体培养和植株再生

In vitro Culture of Grapevine and Datura innoxia Crown Galls Incited by Agrobacterium tumefaciens Biotype 3 Strains and Regeneration of Plants from Tumor Tissues

综述了发根土壤杆菌诱导植物产生毛状根的方法及毛状根的特点，重点介绍了国内利用毛状根培养技术生产植物次生代谢物的最新研究进展。

In this paper the method of hairy-root produced by Agrobacterium rhizogenes inducement was reviewed , and the new research achievements of plant secondary metabolites produced by hairy-root culture technology was introduced .