半乳糖苷

用于B→O血型改造的不同α-半乳糖苷酶的比较

Comparison of Different α - Galactosidases Used in the B to O Blood Conversion

α-半乳糖苷酶是B→O血型改造研究中的关键工具酶。

Galactosidase is the key enzyme in the research of B to O blood conversion .

B→O血型转变工具酶α-半乳糖苷酶cDNA克隆及表达

Cloning and Expression of Alpha-galactosidase cDNA for Seroconversion from Group B to O

制备抗α-半乳糖苷酶单克隆抗体用于检测B→O血型改造后的微量残留酶

Preparation of Monoclonal Antibody Against α - Galactosidase for Detecting Residual Enzyme after B → O Blood Conversion

反义RNA对猪α-1,3-半乳糖苷转移酶活性的影响

The Effect of Antisense RNA on Activity of Porcine α - 1,3-Galactosyltransferase

桃叶片β-半乳糖苷酶基因全长cDNA克隆及原核表达

Cloning and Expression of β - galactosidase Gene cDNA in Peach

该α-半乳糖苷酶反应的最适pH为5.0，最适温度为45℃。

The α - galactosidase has an optimum pH of 5.0 and an optimum temperature of 45 ℃ .

方法：猪囊尾蚴cDNA表达文库中筛选出的β－半乳糖苷糖－猪囊虫cDNA编码的融合蛋白为抗原，进行简化的westernblot分析。

METHODS : Fusion protein of β galactosidase Cysticercus cellulosae cDNA was analysed with simplified Western blot .

目的：建立一种检测B→O血型改造后残留α-半乳糖苷酶的有效方法。

Objective : To set up an effective method specified for detecting minimal amount of residual α - galactosidase in B → O blood conversion process .

人α-半乳糖苷酶、α-1,2-岩藻糖转移酶cDNA序列转染克服异种移植超急性排斥反应

Inhibition of Hyperacute Rejection by Transfection of Human α - Galactosidase cDNA and α - 1,2-Fucosyltransferase cDNA

结果与结论：基因重组α半乳糖苷酶的N端序列与理论序列完全一致；

Results and conclusion : The results showed that the N-terminal amino acids sequence of recombinant α - galactosidase were identical with theoretical sequence completely ;

α半乳糖苷酶因可水解人B型红细胞表面的α半乳糖残基，使B抗原结构变成O抗原结构，而成为B→O血型改造的工具酶。

α - Galactosidase could hydrolyze the α galactose residues on the surface of human blood type B erythrocyte , resulting in the conversion of the B antigen to O antigen .

耐热的β-半乳糖苷酶产生菌株EW－220的分离、鉴定及发酵产物性质的初步研究

Isolation 、 Identification and Products Characterization of Strain EW-220 Producing Thermostable β - galactosidase

β&半乳糖苷酶EA、ED融合蛋白的表达及初步功能研究

Preparing EA and ED of β - galactosidase ( E.coIi ) by fusion protein and its function research

HCV多表位抗原基因与β-半乳糖苷酶基因融合表达、产物纯化及酶活性分析

Expression and Purification of Multi-epitopes Antigen Gene of HCV Fused to β - Galactosidase Gene and Analysis of its Enzyme Activity

离子交换层析纯化出α-半乳糖苷酶，酶比活性从15U／mg提高到28.14U／mg。

The recombinant enzyme was purified by cation exchange chromatography and the activity was up to 28.14U/mg .

短双歧杆菌（Bifidobacteriumbreve203）α-D-半乳糖苷酶的诱导合成及部分酶性质研究

Induction and characterization of α - d-galactosidase ( ec 3.2.1.22 ) from Bifidobacterium breve 203

目的：克隆人α-半乳糖苷酶A（α-galA）cDNA，并在毕赤酵母中表达重组的人α-galA。

Objective : To clone human alpha-galactosidase A (α - Gal A ) cDNA and express recombinant human α - Gal A protein in P.pastoris .

α-半乳糖苷酶消除猪血管内皮Galα（1,3）Gal的初步研究

A Study on Digestion of Gal ( 1,3 ) Gal on Porcine Vascular Endothelial Cells by Galactosidase

目的观察α半乳糖苷酶对猕猴类人B抗原的酶解效果，探讨α半乳糖苷酶酶解对猕猴红细胞结构、功能的影响。

Objective To observe the enzymolysis effect of α - galactosidase on anthropoid human B antigen of the macacas and to explore the impact of enzyme-treatment by α - galactosidase on the structure and function of RBC .

目的观察培养人视网膜色素上皮（RPE）细胞中复制衰老相关的β半乳糖苷酶活性。

Objective To observe the replicative senescence-related beta-galactosidase activity in cultured human retinal pigment epithelium ( RPE ) cells .

目的探讨βD半乳糖苷酶（GAL）和明胶酶B活性在大肠癌黏膜组织中的变化及其与肿瘤进展的关系。

Objective To explore the activity changes of β - D-galactosidase ( GAL ) and gelatinase B in mucosa of colorectal carcinoma and their relationship to tumor progress .

用IPTG（异丙基-硫代-β-D-半乳糖苷）诱导融合蛋白在大肠杆菌中表达；

Fusion protein was expressed in Escherichia coli by the isopropyl thiogalactoside ( IPTG ) induction .

中压液相色谱快速分离纯化Kluyveromycesfragilisβ-D-半乳糖苷酶

Purification of Kluyveromyces fragilis β - Galactosidase by Medium-Pressure Liquid Chromotography

影响Kluyveromycesfragilisβ-D-半乳糖苷酶催化合成低聚半乳糖的因素

Factors Affecting Galacto-oligosaccharide Synthesis by Kluyveromyces Fragilis β - D-galactosidase

11个Fabry病家系的α-半乳糖苷酶A活性及GLA基因检测

Study of alpha-galactosidase A activity detection and gene survey in 11 Chinese pedigrees with Fabry disease

半乳糖苷酶检测结果发现，GCSF受体在酵母细胞中不呈现转录激活功能，然而EPO受体则出现转录激活功能。

The β - galactosidase colony-lift filter assay demonstrated that G-CSF receptor alone had no activity of transcriptional activation , while the EPO receptor alone could activate transcription .

转录因子SP1诱骗性寡核苷酸对猪血管内皮细胞α半乳糖苷链表达的影响

The effect of transcription factor SP1 decoy oligodeoxynucleotides on expression of α Gal in SV-40-PED cells

对转染后24～72h的细胞提取物进行β半乳糖苷酶活性检测；

Preparation of extract from the cells 1 to 3 days post transfection and β galactosidase activity assays were performed .

产β-半乳糖苷酶酵母菌株15D产酶条件研究

Study on the conditions for the production of β - galactosidase by yeast 15D