凝胶过滤色谱

SDS-PAGE电泳和高效凝胶过滤色谱法研究人参蛋白热稳定性

Study on the Thermal Stability of Ginseng Protein by SDS-PAGE and Gel Filtration Chromatography

利用凝胶过滤色谱等手段，对SEA其中含有的蛋白按分子量进行初步分离。

To use Gel Filtration Chromatography to depart the Schistosoma Japanese Egg Antigen according to the molecular weight .

高效凝胶过滤色谱和高效离子交换色谱分析多糖各组分的Mr分布和电荷性质。

High performance gel filtration chromatography and high performance anion exchange chromatography were employed to determine the relative molecular masses and charge characters of each polysaccharides .

并用凝胶过滤色谱测定了不同DE值脂肪替代品的分子量分布，从而在分子水平上探讨胶凝机理。

The molecular weight distributions of components of the fat substitutes with different DE value were measured by GPC , as exploring the mechanism of the gel formation at molecular level .

运用阴离子交换色谱和凝胶过滤色谱从蝮蛇毒中分离出活化C蛋白样酶（APCL）。

Activated protein C like enzyme ( APCL ) was obtained from Agkistrodon halys venom by anion - exchange and gel filtration chromatography .

运用水油提、盐析及凝胶过滤色谱法对幽门螺杆菌YC－11株所分泌的形成空泡细胞毒素进行了提取。

The vacuolating Cytotoxin from Helicobacter pylori YC-11 was purified by using water-extracting , salt precipitation and gel filtration chromatographic fractionation .

研究采用超滤法与凝胶过滤色谱（GFC）相结合实验方法就大豆蛋白酶解产物寡肽混合物的分子量的分布状况进行了测定。

The molecular weight distribution of oligopeptide in soybean protein enzymolysis products was determined through ultrafiltration combined with gel filtration chromatography ( GFC ) .

利用红外光谱法、凝胶过滤色谱法和阳离子交换平衡法，分析了甘氨酸锰的红外光谱特征、甘氨酸锰和蛋氨酸锰在pH7、0.1Mtris缓冲溶液中的络合率。

The percentages of complexed Mn in Mn-Gly and manganese methionine chelate ( Mn-Met ) were determined in 0.1 M Tris buffer ( pH7 ) by gel filtration chromatography and cation exchanging equilibrium .

用吸附色谱法、凝胶过滤色谱法等方法对该活性成分进行分离和纯化，并用高效液相色谱法、薄层色谱法和化学方法对其纯度进行检验，得到2个纯物质HG1和HG2。

The bioactive substances were purified through adsorption chromatography , gel chromatography and so on . The purity tests of HG-1 and HG-2 by TLC , analytical HPLC and chemical methods proved that they were both single substance .

通过干热Maillard反应制备蛋白-多糖共价复合物，并采用凝胶过滤色谱分析反应前后蛋白与多糖Maillard反应产物分子结构特征，证实了SAPP与葡聚糖产生了共价结合；

Protein-polysaccharide conjugates were prepared under drying condition and mild heating . The molecular structure features of SAPP and its conjugated with dextran were analyzed through the Gel Filtration chromatography , the Covalent attachment of polysaccharides to protein was confirmed .

然后利用SephadexG-100凝胶过滤色谱对西兰花籽黑芥子酶进行了纯化，纯化后黑芥子酶的比活力为102U·g-1，比活力是纯化前的5.1倍，且回收率为30%。

Then , broccoli seeds myrosinase was purified to homogeneity by Sephadex G-100 gel filtration chromatography . The specific activity of purified myrosinase was 102U · g-1 , which was 5.1 times than before and the recovery rate of 30 % .

蛋白质凝胶过滤色谱上样方法的改进

An improvement of sample application method in protein gel filtration chromatography

人工伴侣与凝胶过滤色谱耦合作用促进高浓度溶菌酶复性研究

Renaturation of high concentration lysozyme using gel filtration chromatography assisted by artificial chaperones

葡聚糖凝胶过滤色谱柱法测定两种氨基酸锌螯合物螯合率的差异性

Chelation Rate of Zinc Amino Acid Chelate Determined by Sephadex Gel Filtration Chromatography

凝胶过滤色谱法研究酶解过程中木聚糖分子结构的变化

The Changes in the Structure of Xylan during Enzymatic Hydrolysis with Gel Filtration Chromatography

用凝胶过滤色谱技术测定了明胶分子量的分布；

The dis-tribution of molecular weight of gelatin was determined by gel filtration chromatography .

凝胶过滤色谱法测定泥鳅多糖的组成及分子量

Component Analysis and Molecular Weight Determination of Misgurnus Anguillicaudatus Polysaccharides by Gel Filtration Chromatography

采用高效液相凝胶过滤色谱法（HPGFC）测定这3个菌株提取物云芝胞内多糖的相对分子质量，并加以比较。

Their molecular weights were also determined and compared with high performance gel filter chromatography .

对凝胶过滤色谱技术测定壳聚糖分子量的方法和超声波法制备窄分子量分布壳聚糖的工艺进行了研究。

Preparing technique of chitosan with narrow molecular weight distribution through ultrasonic processed was investigated .

高效凝胶过滤色谱法测定玉米蛋白酶解产物寡肽的分子量分布

Assay on the Molecular Weight Distribution of Oligo peptide in Corn Protein Hydrolysate with Gel Filtration Chromatography

凝胶过滤色谱-多角度激光散射法测定琥珀酰明胶自由氨基修饰度

Determination of Modification Degree of Succinylated Gelatin by Size Exclusion Chromatography Coupled with Multi Angle Laser Light Scatting

大豆肽的大孔吸附树脂以及凝胶过滤色谱分离

Isolation and identification of ACE inhibition peptides from soy hydrolytes fraction with macroporous adsorption resin and gel filtration

使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白。

Selenium-containing phycocyanin ( Se-PC ) was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography .

目的：建立高效液相凝胶过滤色谱法测定偶联物的偶联率、中间产物的生成量；

OBJECTIVE To establish a gel filtration chromatography ( GFC ) for determination of conjugation rate and yield of intermediate ;

采用凝胶过滤色谱法研究了木聚糖在酶解过程中分子量分布的变化。

The changes of the molecular weight distributions of xylan during enzymatic hydrolysis have been investigated with gel filtration chromatography .

结论：本实验建立的高效液相凝胶过滤色谱法简便、快速、灵敏、准确，对优化实验有指导意义，使偶联率得到了提高。

CONCLUSION The established method is simple , rapid , sensitive and accurate for optimizing experimental condition and increasing the conjugation rate .

莲藕淀粉及其级分的纯度用其碘络合物的紫外可见扫描图谱与凝胶过滤色谱进行表征。

The purity of lotus root starch and fractions has been confirmed by gel permeation chromatography and UV-Vis scans of their complex formed with iodine .

本课题建立了一种新的蜂毒素提取纯化工艺，单纯采用凝胶过滤色谱法从蜜蜂毒中分离纯化出高纯度蜂毒素；

In this paper , we have established a new technique to extract and purify melittin from bee venom , in which only gel filtration chromatography was used .

本文采用离子交换色谱法及凝胶过滤色谱法对铬鞣废液中络合物组份、电荷、含量和分子大小及其在放置中的变化进行了研究。

The components , charges , molecular sizes and percent contents of complexes in used chrome liquor and their changes in ageing were studied by ion exchange and gel filtration chromatography .

凝胶过滤色谱因其操作方法简单、重复性强等特点在大分子的分离纯化中被广泛使用，特别在基因工程蛋白质类药物的精细纯化中起着难以替代的作用。

Gel filtration chromatography has occupied a key position in the purification of macromolecules for its simplicity and high reproducibility , especially in the polishing purification step of gene engineering protein drugs .