免疫染色

PAP法与PAAP法结合的双重免疫染色技术

A Double Immunostaining Technique of Combination of PAP and PAAP Methods

63例患者Decoy细胞检出率为39.7%，Decoy细胞免疫染色阳性率为54.3%。

Decoy cells were detected in 39.7 % of the 63 patients and the positive rate of decoy cell immunostaining was 54.3 % .

在第7天，在腔上皮和初级蜕膜中仅能够检测较低水平的COX-1免疫染色。

On day 7 , a low level of COX-1 immunostaining was seen in the luminal epithelium and primary decidua .

22～38周，气管内IAPP－IR细胞又呈逐渐减少趋势，IAPP－IR仅出现在基底锥形细胞中（Fig.9＆10），且免疫染色较深。

From the 22nd to 38th week , the number of IAPP-IR cells decreased gradually and IAPP-IR cells appeared in the basal cone-shaped cells with the lighter immunostaining .

方法：用Nissl染色和GFAP免疫染色对脊髓半离断后的大脑皮质运动区神经元和胶质细胞进行了定量和定性研究。

Methods : Quantitative and qualitative study was made on neuron and gliacyte of motor region on pallium by Nissl and GFAP immunochemical staining after half spinal cord broken .

采用14天鸡胚股骨无血清培养、5-溴尿嘧啶核苷（BrdU）掺入标记和细胞核增殖抗原（PCNA）免疫染色方法，研究转化生长因子-β（TGF-β）对骺软骨细胞增殖的作用。

To understand the effect of transforming growth factor beta on epiphyseal chondrocyte proliferation , we detected BrdU incorporation and PCNA expression of epiphyseal chondrocyte of embryonic chick femora which were cultured in serum free and treated with TGF β .

在原始卵泡、初级卵泡、次级卵泡及成熟卵泡的颗粒细胞中都有mPGES免疫染色，其中原始卵泡中最强，初级卵泡、次级卵泡和成熟卵泡中较强。

There was detectable mPGES immunostaining in the granulosa cells of the different follicles . The strongest mPGES immunostaining was seen in the primordial follicles , while a strong level of the immunostaining was also observed in the primary follicles , the secondary follicles , and mature follicles .

免疫染色可见整合素β1在细胞膜上有强表达，并且形成整合素集落。

Integrin β 1 was intensely expressed in the cell membrane , and formed clusters .

细胞免疫染色结果显示，巢蛋白在神经突及生长锥上存在较强的分布，表明它可能参与了有丝分裂后神经元与靶细胞之间神经联系的建立。本文引用了文献19篇。

Moreover , immunostaining showed that nestin was located in the neurite and the growth cone of the P19 neuron , suggesting that nestin might be also involved in the process of the establishment of neural connection .

系统性红斑狼疮伴肝、肾损害时血浆P选择素测定意义免疫金银染色法检测系统性红斑狼疮患者血清中抗ds&DNA抗体的初步研究

Significance of plasma P-selectin detection in systemic lupus erythematosus with liver and kidney lesions Elementary Study of IGSS to Determine Anti-ds-DNA in Sera of patients with SLE

免疫金银染色检测IgM类抗核抗体及临床意义

Detection of ana-igm by immuno - gold-silver staining and its clinical significance

免疫金银染色法在蛋白质微阵列检测TORCH感染的实验研究

Experimental study on detection of TORCH infection using protein microarrays with immuno-gold-silver staining method

采用斑点免疫金银染色法检测了280份临床血清标本的抗RNA抗体。

Anti-RNA antibodies ( ARNA ) were determined by dot-immunogold silver staining ( Dot-IGSS ) in 280 clinical serum samples .

应用DTZ染色、Insulin免疫荧光染色对诱导的细胞进行鉴定。

Differentiated cells were detected by DTZ staining and Insulin immunofluorescence staining .

免疫荧光染色及RT-PCR检测，发现其表达多能性细胞及生殖细胞的标记，而且具有增殖能力，能够向三个胚层的细胞分化。

Immunofluorescence staining and RT-PCR determination found that the cells express pluripotency , proliferation and germ cells ' markers .

应用胶体金探针建立了斑点免疫金染色法（Dot－IGS）。

Ot-immunogold staining ( dot-IGS ) method was established by using colloidal gold probes .

2周时，取肾组织活检，免疫荧光染色，可见IgG沿肾小球毛细血管壁呈连续线形沉积。

At 2nd week , the typical linear deposition of IgG along the glomerular basement membrane ( GBM ) was observed by fluorescence microscopy .

采用碘化丙啶（PropidiumIodide，PI）和免疫荧光染色观察海马脑片的细胞存活和星形胶质细胞的活化增殖。

Hippocampal cells surviving and astrocyte proliferation were detected by propidium iodide ( PI ) and immunofluorescence staining .

上述处理后的细胞经间接免疫荧光染色标记后，用激光共聚焦显微镜观察其细胞膜EGF受体的聚簇现象。

EGF receptors on the cell membrane were ob - served under a laser scanning confocal microscope after indirect immunofluorescence staining .

以FITC标记的羊抗鼠IgG为二抗，建立了间接免疫荧光染色法（IFA）。

Establish the indirect immunofluorescence staining assay ( IFA ) by using goat anti-mouse IgG-FITC as secondary antibody .

HSV-1角膜炎的病因学诊断&免疫酶染色法的应用

Etiologic Diagnosis of HSV-1 Keratitis by Immunoenzymatic Staining

实验方法为：（1）采用免疫荧光染色和流式细胞分析技术检测外周血及灌洗液中CD4~+、CD8~+T细胞和γδT细胞的百分率；

The percentage of CD_4 ~ + T cells , CD_8 ~ + T cells and γδ T cells in PB and BALF were measured by immunofluorescent staining and flow cytometry ;

CD31免疫荧光染色细胞表面呈现黄绿色荧光。

CD 31 antigens cells showed green fluorescence .

采用肝细胞特异性抗体HepPar1进行免疫荧光染色，鉴定和计数分选到的肝癌细胞。

The separated HCC cells were identified by immunofluorescence staining using the hepatocyte-specific antibody Hep Par 1.6 .

以Hep－2细胞作基质的免疫荧光染色表明，4种单抗均呈阳性结果，证实为抗核抗体。

Using Hep-2 cell fluorescent assay , these 4 McAbs were proved to be antinuclear-antibodies ( ANAs ) .

免疫荧光染色显示，AFP、PECAM-1及NF-68三种标志物表达均为阳性。

Immunofluorescent staining showed that AFP , PECAM-1 and NF-68 were positive .

利用CD41单克隆抗体免疫荧光染色观察培养体系中的细胞情况。

CD41 specific monoclonal antibody staining was observed by immunofluorescence microscopy .

方法：选择4种抗突变型P53蛋白抗体，应用免疫金银染色法检测128例人肝细胞癌（HCC）及癌旁组织的表达。

Methods : The expression of mutation P53 protein was observed in the human hepatocellular carcinoma tissue and the tissue nearby carcinoma with immunogold silver staining method in 128 cases .

用抗CD25抗体的免疫荧光染色法识别IL-2α受体的表达，通过3H摄取技术评估重组IL-2对RPE细胞增生的影响。

Immunofluorescent staining was applied to identify the receptor expression using anti-CD25.The effect of recombinant IL-2 on RPE cell proliferation was determined by ~ 3 H uptake .

本实验采用彗星电泳、改进后的胚胎RNA提取方案结合巢式RT-PCR、免疫化学染色的方法，对小鼠体内早期胚胎在发育过程中的细胞凋亡情况及凋亡基因Bcl-2家族的表达情况进行了研究。

We use the comet assay , ameliorative project on getting total RNA from embryos combine the nested RT-PCR , immunochemistry , to analysis apoptosis and the change of Bcl-2 family genes expression .