龙胆

正品龙胆遗传多样性的RAPD及ISSR分析

Analysis of genetic diversity in certified Radix Gentianae by RAPD and ISSR

目的建立龙胆中龙胆苦苷的RPHPLC定量分析方法，并对38批不同产地的龙胆进行含量测定。

Objective To establish a RP-HPLC method for the determination of the content of gentiopicroside in 38 samples of Radix Gentianae from different habitats .

DNA分子标记技术在龙胆鉴别与亲缘关系划分上的应用

Evaluation of the Identification and Phylogeny Relationship among Gentianas by Molecular Markers

HPLC法测定秦艽中龙胆苦苷含量及药动学的研究

HPLC Determination of Gentiana gentiopicrin content and pharmacokinetic study

方法HPLC法测定水煎液和醇沉液中龙胆苦苷的含量；

Methods The contents of Gentiopicroside in water solution and alcohol solution were detected by HPLC .

中药鬼臼毒性成分HPLC/UV指纹图谱分析方法研究及与威灵仙、龙胆HPLC图谱比较

Identification of Podophyllum Species and Radix Clematis and Radix Gentiana by HPLC / UV Fingerprint Analysis

RP-HPLC法测定清咽口服液中龙胆苦苷的含量

Quantitative Determination of Gentiopicroside in Qingyan Oral Solution by RP-HPLC

方法：以龙胆草幼嫩的茎为外植体，培养在MS培养基上，通过胚状体途径获得龙胆草组培苗芽丛。

Method : Tender stems of Gentiana scabra Bunge were cultured as explant in MS medium and clump of buds were acquired via embryoid .

方法采用RP-HPLC测定野生和栽培秦艽中龙胆苦苷的含量。

METHODS The contents of gentiopicroside in wild and planting Gentiana was determinated by RP-HPLC method .

龙胆苦苷在Beagle犬体内药代动力学研究

A Study on Pharmacokinetics Studies of Gentiopicroside in Beagle Dogs

以150μg·mL-1赤霉素变温浸种处理种子，可提高种子发芽率。据此建立了龙胆种子催芽技术和羧甲基纤维素悬浮液播种技术。

The seeds treated with GA3 solution of 150 μ g · mL-1 could increase the germination percentage .

然而，根据STRUCTURE和NJ树的结果分析显示高山龙胆的遗传和表观遗传结构与海拔高度并没有明显的相关性。

However , the results of STRUCTURE and NJ tree revealed that there were no significant correlation between genetic / epigenetic structure and elevation .

目的了解龙胆水提液体外抑制呼吸道合胞病毒（RSV）的作用。

Objective To observe the anti-respiratory syncytial virus ( RSV ) effect of Chinese Gentian .

方法采用HPLC测定在不同烘干温度下干燥的秦艽品中龙胆苦苷的含量。

Methods Using the HPLC to detect the gentiopicroside content at different baking temperature as an index , the suitable baking temperature and the corresponding time were investigated .

并建立了12批坚龙胆药材及饮片的HPLC指纹图谱共有模式，并确定共有峰。

It also establishes the common mode of the HPLC fingerprint of the 12 batches of Gentiana rigescens Franch and their herbs piece and confirms that their common peak .

用滇龙胆的顶芽及侧芽为外植体，以MS培养基为基本培养基，以6-BA/NAA和KT/IAA两组不同浓度组合进行对比实验，研究诱导滇龙胆顶芽及侧芽萌发产生不定芽的最佳条件。

The indefinite bud was induced from terminal bud and lateral bud by using different hormone 6-BA / NAA and KT / IAA in the tissue culture of Gentiana rigescens Franch .

目的建立利胆合剂中环烯醚萜苷类（龙胆苦苷）和蒽醌类（大黄素、大黄酸）的薄层色谱鉴别方法（TLC法）。

Objective To establish the TLC identification method for iridoid glycosides ( gentiopicrin ) and anthraquinone ( emodin , rhein ) in Lidan mixture .

目的：建立应用RP-HPLC测定当药及不同部位中獐牙菜苦苷、当药苷、龙胆苦苷和齐墩果酸的含量测定方法。

Objective : To establish a RP-HPLC method for the determination of swertiamarin , sweroside , gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis .

目的建立龙胆苦苷在Beagle犬血清中的含量测定方法，研究龙胆苦苷在Beagle犬体内的动力学规律。

Objective To establish a method for measuring serum content of gentiopicroside in Beagle dogs and to explore its internal pharmacokinetic features .

考察了温度、pH、缓冲盐种类、离子强度、光照等对龙胆苦苷水溶液稳定性的影响，以确定其最适保存条件。

The effect of temperature , pH , buffer species , buffer concentration , ionic strength and light on the stability of gentiopicrin solution were investigated and the most appropriate preservation conditions for TGG were determined . 4 .

根据本研究的结果显示，高山龙胆具有较高的遗传多样性，其多态位点比率为87.34%，Shannon指数为0.486。

According to our results , the species G. alpina exhibited high genetic diversity . Its percentage of polymorphic bands is 87.34 % and Shannon index is 0.486 .

龙胆抗病毒有效部位RG2-1体外抗RSV作用的实验研究

The anti-respiratory syncytial virus effect of an active compound RG2-1 from Radix Gentianae in vitro

目的研究二妙龙胆合剂的药理及与克拉霉素联合应用治疗非淋菌性尿道炎（黏液脓性宫颈炎）[NGU（MPC）]的临床疗效。

Objectives To study the pharmacologic mechanism of Ermiao-radix-gentianae mixture ( ERM ) and its therapeutic effects associated with clarithromycin in the treatment of nongonococcal urethritis ( mucopurulent cervicitis ) .

结果龙胆泻肝胶囊可抑制SRBC和DNCB致小鼠迟发过敏反应，能提高小鼠血清溶血值，对碳廓清吞噬指数和单核巨噬细胞吞噬功能无影响。

Longdan Xiegan Capsule significantly inhibited DTH allergic reaction induced by DNCB and SRBC in mice , enhanced the hemolysin concentration in mice .

龙胆酸1,2-加双氧酶（GDO）是芳香烃龙胆酸途径的一个关键酶。

Gentisate 1,2-dioxygenase ( GDO ) is the key enzyme of the gentisate pathway .

龙胆草覆盖遮阳网，斑枯病发病时间较裸地栽培晚15d，发病率为23.4%；

Under the treatments with sun-shading net , the occurrence time of septoria leaf spot was delayed 15 days , with the rate of 23.4 % .

方法对二妙龙胆合剂进行体外抗菌试验和毒理试验，并进行联用治疗NGU（MPC）与单用克拉霉素的疗效观察和对比。

Methods ERM 's anti-bacteria and toxicological test were performed in vitro , and the therapeutic effects of ERM plus clarithromycin versus clarithromycin alone in NGU ( MPC ) treatment were also observed .

为控制131I－MIBG内游离131I的含量，研究了自由基清除剂苯甲醇、抗坏血酸、龙胆酸对131I－MIBG分解的抑制作用。

In order to limit the free 131I content , the stability of 131I-MIBG contained different radical scavengers such as benzylalcohol , ascorbic acid and gentisic acid are studied .

从三花龙胆（GentianatrifloraPall.）根部首次分离到一个裂环烯醚萜甙即龙胆苦甙（gentiopicroside），并精制成标准品。

A secoiridoid glucoside , gentiopicroside , has been isolated from the roots of Gentiana triflora Pall . for the first time and purified in to a standard .

产碱假单胞菌NCIB9867株（P25X）能够通过龙胆酸途径降解芳香烃。

Pseudomonas alcaligenes NCIB 9867 ( strain P25X ), a soil bacterium , is capable of degrading aromatic hydrocarbon via the gentisate pathway .