马铃薯葡萄糖琼脂
- 名potato dextrose agar
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病菌生长、产孢的最适培养基为马铃薯葡萄糖琼脂培养基(PDA);
The optimal medium of the pathogen for the mycelium growth and sporulation was PDA .
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方法:1.定期用马铃薯葡萄糖琼脂培养基(PDA)移接豆类丝核菌,使之保持旺盛的生长力。
Methods : 1 . Inoculating Rhizoctonia leguminicola on PDA in a stated time , making it holding hearty vitality .
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2种菌在马铃薯葡萄糖琼脂培养基上生长旺盛,在相对湿度(RH)65%~98%生长良好,且加大RH有利于分生孢子形成,RH100%+水滴处理孢子萌发率最高。
Two pathogens grew well on PDA ( potato glucose agar ) with RH increment at RH 65 % ~ 98 % . Conidium had a highest germination in water drop with full RH .
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采用平板稀释法和植物残渣法,选用马铃薯葡萄糖琼脂培养基(PDA)、孟加拉红琼脂培养基和查氏琼脂培养基(CzA)分别进行菌株的分离、培养与纯化。
Strains were separated , cultured and purified on three different culture media ( PDA , Martin & Johnson Agar and CzA ) by using slab dilution and plant residue method .