非特异性结合

抗体溶液孵育，洗涤后清除非特异性结合。

Antibodies are incubated with the solution , with later washing to remove non-specific binding .

与用较“传统”的方法制备的MIP相比，新聚合物受体的非特异性结合作用减小。

In comparison to a MIP prepared via a more " traditional " approach , the new polymeric receptors exhibit reduced nonspecific binding .

通过比较氢键占有率，我们发现非特异性结合体系的大部分氢键都属于蛋白质和磷酸根之间的氢键，这些氢键对于DNA碱基的直接识别贡献不大。

By comparing the hydrogen bond occupancy , we found that most hydrogen bonds for non-specific complex had a direct interaction with phosphate , and had little contribution to the direct base sequence recognition .

结论：通过对染色条件进行优化可明显提高四聚体染色的特异性，降低非特异性结合，优化的四聚体染色法能用于抗原特异性CTL的表型和功能分析。

CONCLUSION : The optimized tetramer staining condition can increase the specificity of tetramer staining and decrease unspecific binding , therefore it is applicable for phenotyping and functional analysis of antigen-specific CTL .

非特异性结合小于1%。

The non - specific binding was less than 1 % .

并且范德华相互作用是从非特异性结合到特异性识别转变的主要驱动力。

The van der Waals interaction is main driving force for switching from non-specific to specific complex .

[结果]Wistar大鼠脑皮质细胞体外放射受体分析最大结合率369%，非特异性结合103%。

Wistar rat brain cortex membrane radioreceptor binding assay in vitro showed 36.9 % of maximum_binding and 10.3 % of non_specific binding .

充分洗涤，除去非特异性结合抗体，然后用牛血清白蛋白封闭剩余活性位点。

After that , wash it and eliminate the unspecific antibody , use the BSA to seal up the rest points which are not linked .

此结果表明非特异性IgG与支持材料（酶标板）非特异性结合可造成对EIA实验结果的干扰作用。

The results demonstrated that the nonspecific binding of nonspecific IgG with supportive material ( plate ) may interfere with the outcome of EIA .